Generation of a Chinese Hamster Ovary Cell Line Producing Recombinant Human Glucocerebrosidase
Figure 3
Recombinant GCR expression in conditioned media of 3 CHO-DXB11 cell clones selected throughout the stages of amplification, by western blotting analysis. (a) Clone 12, (b) clone 22, and (c) clone 37. Nonglycosylated recombinant GCR of 56 kDa purified from E. coli was the positive control. Glycosylated recombinant GCR is indicated by arrows. The volume of culture medium applied to the gel corresponded to the supernatant of 5,000 cells.
