Generation of a Chinese Hamster Ovary Cell Line Producing Recombinant Human Glucocerebrosidase

Table 1

Expression levels of recombinant GCR secreted from the best CHO-DXB11 cell subclones.

Subclone	Productivity (pg/cell/day)*
12.8	1.84
12.9	5.14
12.19	3.22

*The productivities were calculated by comparing the GCR bands of subclones with a standard curve of non-glycosylated recombinant GCR purified from E. coli, in a western blotting assay followed by densitometric analysis.The experiment was conducted in triplicate.The volume of conditioned medium applied to the gel corresponded to the supernatant of 70,000 cells.