Interferon-α Enhances 5′-Deoxy-5-Fluorouridine-Induced Apoptosis by ERK-Dependant Upregulation of Thymidine Phosphorylase
Figure 1
IFN-α enhanced -DFUR-induced apoptosis in SGC7901 and MGC803 cells. (a) SGC7901 and MGC803 cells were treated with 2.5 μg/mL, 25 μg/mL, 100 μg/mL, 250 μg/mL, or 1250 μg/mL -DFUR for 48 h. The cell viability was examined by MTT assay. (b) SGC7901 and MGC803 cells were preincubated with 1000 IU/mL of IFN-α for 48 h, followed by treatment with 250 μg/mL -DFUR for 48 h. Quantitative representation of the cell viability. Data were means ± SD of three independent experiments. *Incubated with IFN-α and -DFUR versus that with -DFUR alone, . (c) SGC7901 and MGC803 cells were treated as described in (b). Cell apoptosis was quantified with flow cytometry. Data were means ± SD of three independent experiments. *Incubated with IFN-α and -DFUR versus that with -DFUR alone, . (d) SGC7901 and MGC803 cells were treated as described in (b). The expression of caspase-3, caspase-9, and PARP was analyzed by Western blot.
