Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses

Table 3

Diagnostic evaluation of microsphere array assays for HeV detection.

Microsphere array assay; (archival RNA)	HeV qPCR assay^a
	(original diagnostic results)
	Positive	Negative	Indeterminate
Henipa N-gene positive	72	4	4
Henipa N-gene negative	5^b	57	3
Total (𝑛=145)	77	61	7
Henipa P-gene positive	65	2	1
Henipa P-gene negative	12^b	57	6
Total (𝑛=143)^c	77	59	7

Retrospective analysis of results from microsphere array assays on archival RNA of diagnostic submissions in comparison with original qPCR diagnostic results. Preliminary cut-off values (241 MFI for the N-gene and 518 MFI for the P-gene) were derived from results of the negative horse population.
(^a) HeV qPCR negatives include presumed HeV negative samples.
(^b) Five samples categorised HeV qPCR positive in the originally diagnostic assay were negative in both Henipa N- and P-gene assay of archival RNA. All five samples were HeV negative when archival RNA was retested by qPCR (indicating likely degradation of the archival RNA in these samples).
(^c) Two samples from the presumed HeV negative population were not available for the henipa P assay.