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BioMed Research International
Volume 2013 (2013), Article ID 605831, 7 pages
http://dx.doi.org/10.1155/2013/605831
Research Article

Triterpenes and the Antimycobacterial Activity of Duroia macrophylla Huber (Rubiaceae)

1Bioprospection and Biotechnology Laboratory, National Research Institute of Amazonia (INPA), 69060-001 Manaus, AM, Brazil
2Mycobacterial Laboratory, Federal University Foundation of Rio Grande (FURG), 96200-190 Rio Grande, RS, Brazil
3NMR Laboratory, Department of Chemistry, Federal University of Paraná (UFPR), 81530-900 Curitiba, PR, Brazil

Received 9 September 2012; Revised 17 December 2012; Accepted 1 January 2013

Academic Editor: Fabio Ferreira Perazzo

Copyright © 2013 Daiane Martins et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Duroia macrophylla popularly known as “cabeça-de-urubú,” “apuruí,” or “puruí-grande-da-mata” occurs in the Amazon Forest. Its leaves and branches were collected twice and extracted with dichloromethane and methanol. All extracts were subjected to phytochemical investigation and terpenes and flavonoids were found in all dichloromethane and methanol extracts, respectively. Methanol extracts from both branches (1st collection) and leaves (2nd collection) presented hydrolyzed tannins, yet alkaloids were only detected in the dichloromethane and methanol extracts from branches at the 2nd collection. Phenol compounds were found in both dichloromethane extracts’ collections. The action of every extract was assayed against Mycobacterium tuberculosis (RMPr, H37Rv, and INHr strains), showing that the dichloromethane extract from leaves (1st collection) has the major biological activity, with a MIC of 6.25 μg/mL for the INHr strain, 25.0 μg/mL for the RMPr strain, and ≤6.25 μg/mL for the H37Rv strain. The chromatographic fractioning of the dichloromethane extract from leaves (1st collection) yielded the isolation of two triterpenes: oleanolic and ursolic acids, which were identified by NMR analysis and reported for the first time in the Duroia genus.

1. Introduction

Rubiaceae is the largest family in the Magnoliopsida class, encompassing around 550 genera and 9,000 species being used in several ethnomedicinal practices [1]. The family is characterized by the production of several classes of secondary metabolites with a great pharmacological potential, mainly alkaloids, terpenes, quinovic acid glycosides, flavonoids, and coumarins with antibacterial properties [2]. Rubiaceae plants’ secondary metabolites have been investigated scientifically for antimicrobial activities and a large number of plant products have shown to inhibit the growth of pathogenic microorganisms [36]. A literature review article pertaining to Rubiaceae species reveals that 48 out of 611 genera showed a wide range of antibacterial [3] and antimycobacterial activities [4].

The Duroia genus, belonging to Gardenieae tribe and Ixoroideae subfamily, holds about 30 species but few studies have been carried out on this genus. D. hirsuta, which is used as folk healing medicine, is one of these species which has undergone investigation and showed antimycobacterial activity against Mycobacterium phlei [7] and antiviral activity against Herpes simplex viruses (HSV) on in vitro studies [8]. One flavone, one lactone iridoid, one flavonol [9] and one tetracyclic iridoid [10] were isolated from its root petroleum ether and CHCl3 extract. There is still a large number of species with no chemical or biological study.

Duroia macrophylla Huber, popularly known as “cabeça-de-urubú,” “apuruí,” or “puruí-grande-da-mata,” occurs in the Amazon Forest [11]. To the best of our knowledge, no chemical or biological investigations other than ours [12, 13] have been carried out on this species as yet. Hence this work aims to evaluate the antimycobacterial activity of their extracts and isolate and identify the substances present in D. macrophylla active extracts.

2. Material and Methods

2.1. Plant Material

Two collections were performed, the first one, at the “A. Ducke” Forest Reserve, 26 km from Manaus, was carried out on December 5th, 2008, and a voucher specimen (222383) was deposited at the Herbarium of the Botanical Research Coordination of the National Research Institute of Amazonia (INPA). The second one at the Natural Heritage Private Reserve, locally known as “Cachoeira da Onça,” in “Presidente Figueiredo” County, AM, was carried out on May 18th, 2011. A voucher specimen (222501) was deposited at the same Herbarium.

2.2. Extracts Preparation

Plant material (leaves and branches) was dried in an oven at 50°C and powdered. Each plant part was extracted three times separately, first with dichloromethane (DCM) followed by methanol (MeOH), in a sonic bath for 20 minutes. After filtration, DCM and MeOH extracts were concentrated under reduced pressure.

2.3. Phytochemical Investigation

The extracts were analyzed following the methodology described by Matos [14], as well as by thin layer chromatography (TLC) (Merck) using silica with UV254 fluorescence detector on aluminum support, eluted with appropriated systems, and revealed with UV light (λ = 254 and 365 nm), sulfuric p-anisaldehyde, Ce(SO4)2,   2,2-diphenyl-1-picrylhydrazyl (DPPH), FeCl3, and Dragendorff. Chemical extract profile was identified through 1H-NMR on an Anasazi NMR spectrometer operating at 1.4 Tesla (60 MHz).

2.4. Extract Fractionation

Dichloromethane extract from leaves (1st collection) (9 g) was submitted to a chromatographic column (CC) fractionation on silica gel (332 g), eluted with gradients of hexane/ethyl acetate and ethyl acetate/methanol, yielding 99 fractions with 50 mL each. Fraction 25–40 (900 mg) was fractionated on silica gel (90 g) CC and eluted with hexane/ethyl acetate and ethyl acetate/methanol gradients, yielding 42 fractions with 20 mL each. Fraction 25–40.6 (130 mg) was fractionated on silica gel (17 g) CC and eluted with hexane/ethyl acetate and ethyl acetate/methanol gradients, yielding 19 fractions with 10 mL each. Afterwards, fraction 25–40.6.4 (4 mg) was submitted to high-performance liquid chromatography (HPLC) analysis. HPLC was performed with a Shimadzu system SCL-10AVP, processing software programs CLASS VP, dual LC-6AD pumps, 10AF autosampler, SPD-M20 diode-array detector, cyanopropyl column (  mm, 4 μm particle sizes, Luna-Phenomenex), with acetonitrile : water (90 : 10) as the isocratic mobile phase, at a 5 mL/min flow rate. The injection volume was 35 μL. The resolved peaks retention times were 11.5 and 12 min, identified by NMR analyses as oleanolic acid (1) and ursolic acid (2), respectively (Figure 1).

605831.fig.001
Figure 1: Schematic representation of D. macrophylla dichloromethane extract of leaves (1st collection) fractionation. DCM: dichloromethane; CC: chromatographic column; HPLC: high-performance liquid chromatography.

All fractions were evaluated by TLC analysis, eluted with appropriated systems, and revealed under UV light exposure (λ = 254 and 365 nm), sulfuric p-anisaldehyde, Ce (SO4)2,   2,2-diphenyl-1-picrylhydrazyl (DPPH), FeCl3, and Dragendorff reagents.

2.5. NMR Data

The NMR data was obtained at 295 K on a Bruker AVANCE 400 NMR spectrometer operating at 9.4 Tesla, observing 1H and 13C at 400 and 100 MHz, respectively. The spectrometer was equipped with a 5 mm multinuclear direct detection probe, with z-gradient. One-bond (HSQC) and long-range (HMBC) 1H-13C NMR correlation experiments were optimized for coupling constants and of 140 and 8 Hz, respectively. All NMR chemical shifts were expressed in ppm related to TMS signal at 0.00 ppm as internal reference, and samples were dissolved in CDCl3.

2.6. Antimycobacterial Activity

Resazurin microtiter assay (REMA) was used to evaluate the antimycobacterial activity. This method uses resazurin as an oxidoreduction indicator to evaluate the bacterial viability and contamination, in addition to analyzing the antimicrobial activity [15].

2.6.1. Microorganisms

The extracts activity was evaluated against three Mycobacterium tuberculosis strains: one pan-sensible (H37Rv, ATCC 27294), one isoniazid monoresistant (INH, ATCC 35822) with mutation in katG, codon S315T (AGC-ACC), and other rifampicin monoresistant (RMP, ATCC 35338), with mutation in rpoB, codon H526T (CAC-TAC). The strains were cultivated in Ogawa-Kudoh’s medium at 37°C for nearly 14 days. The bacterial suspension of each strain was prepared in a sterile tube with glass pearls and turbidity adjusted with distillated water, according to Mc Farland scale’s number 1 tube, which corresponds to approximately  CFU/mL. Then, Middlebrook 7H9 medium was added to bacterial suspension in 1 : 20 ratio [15].

2.6.2. Assay Procedure

Samples were first evaluated in 96-well microplates at a 200 μg/mL concentration against the three M. tuberculosis strains. The assay started adding 75 μL of Middlebrook 7H9 medium enriched with 10% of OADC (oleic acid, albumin, dextrose, and catalase) for M. tuberculosis, 75 μL of each extract, and 75 μL of inoculum. Then, 200 μL of sterile water were added to each peripheral well, so as to avoid medium liquid evaporation when heater-incubated. Finally, the plate was incubated at 37°C for seven days.

2.6.3. Minimum Inhibitory Concentration Determination

The extracts presenting an antimycobacterial activity at the 200 μg/mL concentration screening were chosen to evaluate their minimum inhibitory concentration (MIC) value [16]. This value was determined by adding 100 μL of medium, 100 μL of extract (starting at 200 μg/mL concentration on the first well and performing a 1 : 2 microdilution), and 100 μL of bacterial inoculum in each well. Also, 200 μL of sterile water was added to each peripheral well, in order to avoid medium liquid evaporation when heater-incubated. Then, the plate was incubated at 37°C for seven days.

2.6.4. Bacterial Viability

Following the incubation period, 30 μL of resazurin (0.02%) was added in each well and incubated for two days at 37°C. The biological activity was based on the color change, from blue to pink when an oxidoreduction reaction of the reagent occurs due to bacterial growth [15].

3. Results and Discussion

3.1. Phytochemical Investigation

All Duroia macrophylla extracts were analyzed in order to evaluate the chemical profile [17, 18]. Dichloromethane extracts from branches and leaves in both collections showed to be rich in terpenes. Regarding methanolic extracts, only those from branches (1st collection) and leaves (2nd collection) showed the presence of both terpenes and hydrolyzed tannins. Alkaloids were only detected on dichloromethane and methanolic extracts from branches (2nd collection). All methanolic extracts showed the presence of flavonoids. All dichloromethane extracts from branches showed the presence of phenolic compounds. 1H-NMR spectra analysis showed the presence of aromatic substances in the methanolic extract of branches (1st collection), with several signals between 6.50 and 7.80 ppm.

3.2. Substances Isolation and Identification

Following crude extracts chemical and biological analysis, the dichloromethane extract from leaves (1st collection) was chosen to be fractionated, since it showed to be the most active against the three Mycobacterium tuberculosis strains (RMPr, H37Rv and INHr) (Table 2).

Fraction 25–40.6 1H-NMR data showed the presence of several signals in the shielded region between 0.7 and 1.2 (s), characteristic of methyl hydrogens; two signals at 5.31 (dd, ; 3.5 Hz) and 5.27 (dd, ; 3.5 Hz) characteristic of olefinic hydrogen, and also two signals at 3.23 (dd, ; 4.7 Hz) and 3.22 (dd, ; 4.9 Hz) which agree with carbinolic hydrogens. All this data suggests the mixture of two triterpenes.

HPLC fractionation of this mixture was performed in order to isolate them, and yielded two fractions, 1 and 2, with retention times of 11.5 and 12.0 min. The 1H-NMR spectrum from fraction 25–40.6.4.1 showed the signal at 5.31 (dd, ; 3.5 Hz) and from fraction 25–40.6.4.2, the signal at 5.27 (dd, ; 3.5 Hz).

The substance 1  1H-13C NMR (HSQC) correlation map showed the hydrogen at 5.31 ppm with the carbon at 122.8, which were identified as the vinilic C-12 carbon of oleanolic acid [7, 19] (Table 1). The signal in 180.0 was assigned to the carboxyl group (C-28).

tab1
Table 1: 1H and 13C NMR chemical shifts ( , ppm) data of oleanolic and ursolic acids (400 MHz, CDCl3).
tab2
Table 2: Minimum inhibitory concentration (MIC) of D. macrophylla extracts against M. tuberculosis strains.

The 1H-NMR spectrum from fraction 25–40.6.4.2 showed several signals at the shielded region, between 0.79 and 1.72 characteristic of methyl hydrogens, moreover two signals were observed at 3.22 (dd, and 4.9 Hz) and 5.27 (dd, and 3.5 Hz) characteristic of olefinic hydrogen, which were assigned to H-12 in triterpenes, characterizing the ursanic skeleton of substance 2.

When analyzing the 13C-NMR spectral data one can find seven methyl carbons (CH3), nine methylene carbons (CH2), seven methine carbons (CH) and seven non-hydrogenated carbons (C), resulting in thirty carbons characteristic of pentacyclic triterpenes. 179.6 from the carboxylic acid carbon (not hydrogenated), 137.9 characteristic of unhydrogenated olefinic carbon (sp2) and 125.9 of hydrogenated olefinic carbon are the major signals characteristic of a ursanic skeleton. These signals represent, carbons C-28, C-13 and C-12 in ursolic acid triterpene, respectively.

On the other hand, the 1H-13C NMR (HSQC) correlation map showed correlation of the hydrogen at 5.27 ppm with the carbon at 125.9, which were identified as the vinilic C-12 carbon and the multiplicity of the signals corresponding to H-18 and related CH3-29 and CH3-30 determined the ursolic acid.

In the two-dimensional 1H-1H NMR (COSY) correlation map, the following correlations are observed: hydrogen H-11 (δ 1.91) with H-12 ( 5.27).

It is common to isolate the ursolic acid with oleanolic acid mixture due to molecule similarity, yet a few differences between them enable telling them apart through NMR, due to the difference between the H-18, C-18, C-12, C-13 and C-29 [20] chemical shifts, and mainly on account of H-29 being a doublet for ursolic acid, and a singlet for oleanolic acid.

1H-NMR spectra and HSQC and HMBC NMR correlation maps overall analysis as well as comparison with literature data [7] enabled the complete structure to be determined as the triterpenes oleanoic and ursolic acids (Table 1) (Figure 2).

605831.fig.002
Figure 2: Structures of oleanolic and ursolic acids and their 1H-13C long-range correlations.

The mass spectra analysis of each triterpene isolated showed the molecular ion peak at  u, and showed the common fragmentation pattern of triterpenes, described in the literature [21]. All these data together confirmed to be the triterpenes, ursolic acid and oleanolic acid. To the best of our knowledge, this is the first report of these two triterpenes in Duroia genus.

3.3. Antimycobacterial Activity

All extracts showed activity against M. tuberculosis at least for one strain, except for the methanol extract of branches (1st collection) (Table 2). The dichloromethane extract of leaves (1st collection) showed the highest activity, with a MIC of 6.25 μg/mL for INHr strain, 25.0 μg/mL for RMPr strain and ≤6.25  g/mL for H37Rv strain. Triterpenes oleanoic and ursolic acids were isolated from this extract. The methanolic extract of leaves (2nd collection) that showed a MIC of 12.5  g/mL for INHr strain was the second most active one (Table 2).

The wide variety of natural products chemical structures plays a major role on the development of new antimycobacterial drugs generations, as shown in the extensive literature revision made by Copp [22], from 1990 to 2002, which uncovered 352 substances isolated from natural products (terrestrial and marine) presenting an antimycobacterial activity and a MIC ≤ 64 μg/mL.

The highest activity of the dichloromethane extract from leaves (1st collection) in this work could be attributed to the presence of terpenes. Several studies, such as those performed by Newton et al. [23], Cantrell et al. [24], Copp [22], Seidel and Taylor [25], Aguiar et al. [26], and Higuchi et al. [27], showed terpenes to be responsible for the antimycobacterial activity.

Extracts and compounds from other Rubiaceae species, such as Duroia hirsuta and Psychotria vellosiana, showed activity against Mycobacterium phlei [6] and M. tuberculosis and M. kansasii, respectively [28]. According to some authors, the antimycobacterial activity can also be related to the presence of alkaloids, normally found in Rubiaceae species [29, 30].

Out of the 27 assayed fractions present in this work, only fraction 63-65 was as active against M. tuberculosis INHr strain as the dichloromethane extract of leaves (1st collection) (MIC of 25 μg/mL). Among the others, 15 fractions were active against M. tuberculosis H37Rv strain (MIC between 50 and 200 μg/mL), 17 fractions were active against INHr strain (MIC between 25 and 200 μg/mL), and 16 fractions were active against RMPr strain (MIC between 50 and 200 μg/mL) (Table 3). Fraction 25-40.6 was active against the three strains, with a MIC of 200 μg/mL and their fractionation yielded the substances 1 and 2 corresponding to the triterpenes oleanoic and ursolic acids, respectively.

tab3
Table 3: Minimum inhibitory concentration (MIC) of dichloromethane fractions from the leaves of D. macrophylla (1st collection) against M. tuberculosis strains.

Studies conducted by Higuchi et al. [27] reported the oleanolic and ursolic acids’ mixture MIC to be 62.5 μg/mL. Other studies showed the growth inhibitory activity against Mycobacterium tuberculosis with a MIC of 16 μg/mL for oleanolic acid and 50 μg/mL for ursolic acid [24].

Ge et al. [31] also obtained a better MIC value for oleanolic acid (MIC of 28.7 μg/mL) than that for ursolic acid (MIC of 41.9 μg/mL). These latter examples showed each isolated compound to exhibit a better MIC than the mixture’s.

The high lipophilicity of terpenes is probably the main factor that allows their penetration through the mycobacterial cell wall [27].

Other studies showed these substances inhibited 99% the growth of M. tuberculosis H37Rv [32]. The literature data reported that oleanolic acid has a synergistic effect when combined with isoniazid, rifampicin, or ethambutol (first line antitubercular drugs) [32].

According to Pauli et al. [33], a crude extract MIC may or not be a reliable antimycobacterial activity indicator since such extracts could hold active compound antagonist substances decreasing the MIC. Otherwise, a crude extract could hold compound agonists with increasing effects on MIC, the so called synergism effect. According to the author an extract with high activity (lower MIC) could present large amounts of compounds with moderated antimycobacterial activity. In other scenario, crude extracts with moderated MIC could hold small amounts of chemically active compounds. When the extract loses its activity during the fractionation, it could be due to a synergism effect between the substances on it. Therefore, the combined action of two or more substances can result on a biological effect higher than any single one’s. Hence, more thorough studies are necessary to find which substances should be mixed in order to attain the desirable antimycobacterial activity.

Acknowledgments

The authors are grateful to CT-Agro/CNPq/MCTI (520281/2007-1 and 562892/2010-9), PPBio/CNPq/MCTI (558321/2009-7), and CENBAM/CNPq/MCTI for the financial support.

References

  1. E. Robbrecht, “Tropical woody Rubiaceae,” Opera Botanica Belgica, vol. 1, no. 272, pp. 599–602, 1988.
  2. M. E. Heitzman, C. C. Neto, E. Winiarz, A. J. Vaisberg, and G. B. Hammond, “Ethnobotany, phytochemistry and pharmacology of Uncaria (Rubiaceae),” Phytochemistry, vol. 66, no. 1, pp. 5–29, 2005. View at Publisher · View at Google Scholar · View at Scopus
  3. K. D. Choudhury, M. D. Choudhury, and M. Baruah, “Antibacterial activity of some plants belonging to the family Rubiaceae: a review,” World Journal of Pharmacy and Pharmaceutical Sciences, vol. 1, no. 3, pp. 1170–1192, 2012.
  4. T. M. D. S. Moraes, M. H. de Araújo, N. R. Bernardes et al., “Antimycobacterial activity and alkaloid prospection of Psychotria species (Rubiaceae) from the Brazilian Atlantic Rainforest,” Planta Medica, vol. 77, no. 9, pp. 964–970, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. K. A. Muthu and C. D. Shajiselvin, “In vitro evaluation of free radical scavenging potential of various extracts of whole plant of Borreria hispida (Linn),” International Journal of Pharmaceutical Sciences and Research, vol. 1, no. 2, pp. 127–130, 2010.
  6. R. Usha, S. Sashidharan, and M. Palaniswami, “Antimicrobial activity of a rarely known species Morinda citrifolia L.,” Ethnobotanical Leaflets, vol. 14, pp. 306–311, 2010.
  7. A. Lopez, J. B. Hudson, and G. H. N. Towers, “Antiviral and antimicrobial activities of Colombian medicinal plants,” Journal of Ethnopharmacology, vol. 77, no. 2-3, pp. 189–196, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. M. T. H. Khan, A. Ather, K. D. Thompson, and R. Gambari, “Extracts and molecules from medicinal plants against herpes simplex viruses,” Antiviral Research, vol. 67, no. 2, pp. 107–119, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. R. Aquino, N. de Tommasi, M. Tapia, M. R. Lauro, and L. Rastrelli, “New 3-methyoxyflavones, an iridoid lactone and a flavonol from Duroia hirsuta,” Journal of Natural Products, vol. 62, no. 4, pp. 560–562, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. J. E. Page, S. Madriñán, and G. H. N. Towers, “Identification of a plant growth inhibiting iridoid lactone from Duroia hirsuta, the allelopathic tree of the “Devil’s garden”,” Experientia, vol. 50, no. 9, pp. 840–842, 1994. View at Publisher · View at Google Scholar · View at Scopus
  11. J. E. L. S. Ribeiro, M. J. G. Hopkins, A. Vicentini et al., Flora da Reserva Ducke. Guia de identificação das plantas em uma floresta de terra firme na Amazônia central, Instituto Nacional de Pesquisas da Amazonia (INPA) and Department for International Development (DFID), Manaus, Brazil, 1999.
  12. C. V. Nunez, P. A. Santos, V. Roumy et al., “Raunitidine isolated from Duroia macrophylla (Rubiaceae),” Planta Medica, vol. 75, no. 9, pp. 1037–1037, 2009.
  13. C. V. Nunez, V. Roumy, D. W. O. Mesquita et al., “Indole alkaloids from Duroia macrophylla (Rubiaceae),” Planta Medica, vol. 78, no. 11, pp. 1209–1209, 2012.
  14. F. J. A. Matos, Introdução à Fitoquímica Experimental, EUFC, Fortaleza, Brazil, 1997.
  15. J. C. Palomino, A. Martin, M. Camacho, H. Guerra, J. Swings, and F. Portaels, “Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis,” Antimicrobial Agents and Chemotherapy, vol. 46, no. 8, pp. 2720–2722, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. S. G. Franzblau, R. S. Witzig, J. C. Mclaughlin et al., “Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay,” Journal of Clinical Microbiology, vol. 36, no. 2, pp. 362–366, 1998. View at Scopus
  17. M. Abreu, Triagem fitoquímica de plantas por cromatografia em camada delgada (CCD), Apostila de Curso, Laboratorio de Fitoquímica, Escola de Farmácia, Universidade de Ouro Preto, 2000.
  18. H. Wagner and S. Bladt, Plant Drug Analysis: A Thin Layer Chromatography Atlas, Springer, New York, NY, USA, 2001.
  19. W. Seebacher, N. Simic, R. Weis, R. Saf, and O. Kunert, “Complete assignments of 1H and 13C NMR resonances of oleanolic acid, 18α-oleanolic acid, ursolic acid and their 11-oxo derivatives,” Magnetic Resonance in Chemistry, vol. 41, no. 8, pp. 636–638, 2003. View at Publisher · View at Google Scholar · View at Scopus
  20. V. G. Kontogianni, V. Exarchou, A. Troganis, and I. P. Gerothanassis, “Rapid and novel discrimination and quantification of oleanolic and ursolic acids in complex plant extracts using two-dimensional nuclear magnetic resonance spectroscopy—comparison with HPLC methods,” Analytica Chimica Acta, vol. 635, no. 2, pp. 188–195, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. I. Ogunkoya, “Application of mass spectrometry in structural problems in triterpenes,” Phytochemistry, vol. 20, no. 1, pp. 121–126, 1981. View at Scopus
  22. B. R. Copp, “Antimycobacterial natural products,” Natural Product Reports, vol. 20, no. 6, pp. 535–557, 2003. View at Publisher · View at Google Scholar · View at Scopus
  23. S. M. Newton, C. Lau, and C. W. Wright, “A review of antimycobacterial natural products,” Phytotherapy Research, vol. 14, no. 5, pp. 303–322, 2000. View at Publisher · View at Google Scholar
  24. C. L. Cantrell, S. G. Franzblau, and N. H. Fischer, “Antimycobacterial plant terpenoids,” Planta Medica, vol. 67, no. 8, pp. 685–694, 2001. View at Scopus
  25. V. Seidel and P. W. Taylor, “In vitro activity of extracts and constituents of Pelagonium against rapidly growing mycobacteria,” International Journal of Antimicrobial Agents, vol. 23, no. 6, pp. 613–619, 2004. View at Publisher · View at Google Scholar · View at Scopus
  26. R. M. Aguiar, J. P. David, and J. M. David, “Unusual naphthoquinones, catechin and triterpene from Byrsonima microphylla,” Phytochemistry, vol. 66, no. 19, pp. 2388–2392, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. C. T. Higuchi, F. R. Pavan, C. Q. F. Leite et al., “Triterpenes and antitubercular activity of Byrsonima crassa,” Quimica Nova, vol. 31, no. 7, pp. 1719–1721, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. D. F. Ramos, G. G. Leitão, F. N. Costa et al., “Investigation of the antimycobacterial activity of 36 plant extracts from the brazilian Atlantic Forest,” Revista Brasileira de Ciências Farmacêuticas, vol. 44, no. 4, pp. 669–674, 2008. View at Publisher · View at Google Scholar
  29. J. Schripsema, A. Ramos-Valdivia, and R. Verpoorte, “Robustaquinones, novel anthraquinones from an elicited Cinchona robusta suspension culture,” Phytochemistry, vol. 51, no. 1, pp. 55–60, 1999. View at Publisher · View at Google Scholar · View at Scopus
  30. M. R. Khan and A. D. Omoloso, “Antibacterial and antifungal activities of Angiopteris evecta,” Fitoterapia, vol. 79, no. 5, pp. 366–369, 2008. View at Publisher · View at Google Scholar · View at Scopus
  31. F. Ge, F. Zeng, S. Liu et al., “In vitro synergistic interactions of oleanolic acid in combination with isoniazid, rifampicin or ethambutol against Mycobacterium tuberculosis,” Journal of Medical Microbiology, vol. 59, no. 5, pp. 567–572, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. A. Jiménez, M. Meckes, V. Alvarez, J. Torres, and R. Parra, “Secondary metabolites from Chamaedora tepejilote (Palmae) are active against Mycobacterium tuberculosis,” Phytotherapy Research, vol. 19, no. 4, pp. 320–322, 2005. View at Publisher · View at Google Scholar · View at Scopus
  33. G. F. Pauli, R. J. Case, T. Inui et al., “New perspectives on natural products in TB drug research,” Life Sciences, vol. 78, no. 5, pp. 485–494, 2005. View at Publisher · View at Google Scholar