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BioMed Research International
Volume 2013 (2013), Article ID 804632, 8 pages
http://dx.doi.org/10.1155/2013/804632
Research Article

Human Resistin Inhibits Myogenic Differentiation and Induces Insulin Resistance in Myocytes

1Department of Central Research, The Third Clinical College, Jilin University, Changchun, Jilin 130033, China
2Cell Transplantation Center, The 208th Hospital of Chinese People's Liberation Army, Changchun, Jilin 130021, China

Received 21 August 2012; Revised 6 December 2012; Accepted 21 December 2012

Academic Editor: Joseph Fomusi Ndisang

Copyright © 2013 Chun Hua Sheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study is aimed to investigate the effect of human resistin on myocyte differentiation and insulin resistance. The human resistin eukaryotic expression vector was stable transfected into C2C12 myocyte cells and was transiently transfected into COS7 cells. The effects of human resistin on cell proliferation, cell cycle, and myogenic differentiation of C2C12 cells were examined. Glucose uptake assays was performed on C2C12 myotubes by using [3H] 2-deoxy-D-glucose. The mRNA levels of insulin receptor (IR) and glucose transporter 4 (GLUT4) were evaluated by semiquantitative RT-PCR. Results showed by the C2C12 cells transfected with human resistin gene compared with that without transfecting gene are as follows: (1) cell proliferation was significantly promoted, (2) after inducing differentiation, the myotube’s diameters and expression of desmin and myoglobin decreased, and (3) glucose uptake ratio was lowered and expression of IR and GLUT4 decreased. However, there was no significant difference in the glucose uptake ratio between C2C12 myotubes treated with a human resistin conditioned medium of COS7 cells and treated with control medium. These results suggest that maybe human resistin has not a direct role on insulin sensitivity of myocytes. However, maybe it impaired the insulin sensitivity of myocytes through suppressing myogenesis and stimulating proliferation of myoblasts.