Inhibition of Nek2 by Small Molecules Affects Proteasome Activity
Figure 2
Nek2 overexpression elevates the proteasome activity in multiple cancer cell lines. (a) Proteasome activity is significantly increased in Nek2 overexpressed HeLa cells compared to GFP-transfected control. Proteasome activity was also significantly elevated in H929 (b), KMS28PE (c), and ARP-1 (d) cell lines compared to empty vector transfected (control). For the ARP-1 cell line, Nek-2-OE, NEK-2-KD, and bortezomib-resistant clones were tested in addition to wild-type cells. The 26S proteasome was isolated by ultracentrifugation and the proteasome activity was determined by Proteasome-Glo Assay. (d) For ARP1 cells, the bortezomib-resistant cells (third column in (d)) showed higher proteasome activity. For Figures 2(a)–2(d), , .
