Biological and Molecular Effects of Small Molecule Kinase Inhibitors on Low-Passage Human Colorectal Cancer Cell Lines
Figure 3
Effects of vemurafenib, trametinib, perifosine, and regorafenib on the phosphorylation of AKT, MEK1/2, and ERK1/2 in CRC cell lines. (a) HROC18, (b) HROC24, (c) HROC43, and (d) HROC46 cells were grown in 24-well plates to subconfluency before culture medium was supplemented with vemurafenib, trametinib, perifosine, and regorafenib at the indicated concentrations. Control cultures (C) were treated with solvent only. After an incubation period of 6 h, protein extracts from equal amounts of cells were subjected to immunoblot analysis. P-AKT, P-MEK1/2, P-ERK1/2, the respective total proteins, and GAPDH (for loading control) were detected using fluorescein- (IRDye-) labeled secondary antibodies. For each cell line, one representative blot is shown. For mean values of independent experiments, please refer to Figure 4.
