The Histone Acetyltransferase Gcn5 Regulates ncRNA-ICR1 and FLO11 Expression during Pseudohyphal Development in Saccharomyces cerevisiae

Figure 4

Gcn5 regulates transcription of ICR1 and FLO11 in response to 3AT. (a) A schematic representation of the transcription initiation sites and direction of ncRNAs ICR1 and PWR1 at the FLO11 promoter (adapted from [43]). (b) RT-qPCR analysis of expression of ncRNA ICR1 and FLO11. Haploid (two top panels) or diploid (two bottom panels) wild type (WT) and gcn5 strains were treated with or without 10 mM 3AT. Cells were cultured to early log phase in minimal medium and induced by 3AT for 2 h (haploids) or 1 h (diploids). In total, 12 sets of strand-specific primers tiled from −15 bp to −3 kb upstream of the FLO11 promoter. The transcript levels were normalized to that of ACT1. The signal at −3 kb of the FLO11 promoter in wild type was set to 1. (c) Quantification of transcript levels of ICR1, ICR1/PWR1, and FLO11 in diploids treated with or without 3AT. Data are derived from Figure 4(b) (two bottom panels) and the transcript levels at specified locations are indicated in parentheses. Data are mean ± SD from 3 biological repeats. ; ; by Student’s -test.

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