Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice
Table 3
Quantification of apoptosis.
Cell status
Control
24 hours
Control
48 hours
p-PD (µg/mL)
p-PD (µg/mL)
20
40
100
20
40
100
A375
Live
92 ± 1
90 ± 1.3
89 ± 4.34
81 ± 0.34
91 ± 0.85
59 ± 1.3
42 ± 0.23
36 ± 0.6
Early apoptosis
3 ± 0.2
3 ± 0.5
5 ± 0.3
11 ± 0.19
6 ± 0.93
11 ± 0.4
17 ± 0.2
21 ± 0.58
Late apoptosis
4 ± 0.4
6 ± 0.4
6 ± 0.3
7 ± 0.43
2 ± 0.1
27 ± 0.3
39 ± 0.5
40 ± 0.86
Sub-G0/G1
10 ± 1.8
11 ± 0.5
15 ± 2.2
24 ± 4
10 ± 2.8
38 ± 6.4
39 ± 5.1
50 ± 7.3
B16-F10
Live
87 ± 6.8
75 ± 7.4
73 ± 6.6
71 ± 6.8
92 ± 4.1
44 ± 5.3
36 ± 2.4
8 ± 1
Early apoptosis
6 ± 0.3
16 ± 3.2
17 ± 3.4
17 ± 2.2
4 ± 0.8
32 ± 4.1
36 ± 3.1
42 ± 1.9
Late apoptosis
5 ± 0.8
7 ± 2
9 ± 3
11 ± 1.6
4 ± 0.6
21 ± 1.9
23 ± 2.5
43 ± 1.8
Sub-G0/G1
1 ± 1.5
4 ± 1
6 ± 1.4
10 ± 1.2
2 ± 0.1
22 ± 3.1
55 ± 4.4
60 ± 2.9
The percent cell numbers of early and late apoptotic stages were determined from the respective dot plots (one such plot is shown in Figure 3(b)). The sub-G0/G1 cells were estimated using standard cell cycle analysis by flow cytometry. All the sets were performed in triplicate.
p-PD treated and untreated (control) A375 andB16-F10 cells were subjected to Annexin V-FITC assay as mentioned in the Materials and Methods.
