Research Article
Mass Spectrometry of Putrescine, Spermidine, and Spermine Covalently Attached to Francisella tularensis Universal Stress Protein and Bovine Albumin
Table 1
Neutral masses of polyamine adducts on glutamate, glutamine, and aspartate.
| Polyamine | Amino acid residue | Catalyst | Dehydro residue | Polyamine added mass | Adduct neutral mass |
| Putrescine | Glutamate | EDC | 129.04 | 70.06 | 199.10 | Putrescine | Glutamine | Transglutaminase | 128.05 | 71.07 | 199.12 | Spermidine | Glutamate | EDC | 129.04 | 127.12 | 256.16 | Spermidine | Glutamine | Transglutaminase | 128.05 | 128.13 | 256.18 | Spermine | Glutamate | EDC | 129.04 | 184.18 | 313.22 | Spermine | Glutamine | Transglutaminase | 128.05 | 185.19 | 313.24 | Putrescine | Aspartate | EDC | 115.02 | 70.06 | 185.08 | Spermidine | Aspartate | EDC | 115.02 | 127.12 | 242.14 | Spermine | Aspartate | EDC | 115.02 | 184.18 | 299.20 |
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There are no entries in Table 1 for transglutaminase adducts on glutamate or aspartate because transglutaminase only labels glutamine. |