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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 408187, 12 pages
http://dx.doi.org/10.1155/2012/408187
Research Article

Oroxylin A, but Not Vasopressin, Ameliorates Cardiac Dysfunction of Endotoxemic Rats

1Department of Life Sciences, College of Life Sciences, Tzu Chi University, No. 701, Section 3, Chung Yan Road, Hualien, Taiwan
2Center for Vascular Medicine, College of Life Sciences, Tzu Chi University, No. 701, Section 3, Chung Yan Road, Hualien, Taiwan
3Department of Research, Buddhist Tzu Chi General Hospital and Tzu Chi College of Technology, Hualian, Taiwan
4Medical Research, College of Medicine, Tzu Chi University, No. 701, Section 3, Chung Yan Road, Hualien, Taiwan
5Graduate Institute of Biomedical and Biopharmaceutical Sciences, College of Life Sciences, National Chiayi University, Chiayi, Taiwan
6Institute of Pharmacology and Toxicology, College of Medicine, Tzu Chi University, No. 701, Section 3, Chung Yan Road, Hualien, Taiwan
7Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, IL, USA

Received 9 August 2012; Accepted 13 September 2012

Academic Editor: Waris Qidwai

Copyright © 2012 Chin-Hung Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The mortality in septic patients with myocardial dysfunction is higher than those without it. Beneficial effects of flavonoid oroxylin A (Oro-A) on endotoxemic hearts were evaluated and compared with that of arginine vasopressin (AVP) which is used to reverse hypotension in septic patients. Endotoxemia in rats was induced by one-injection of lipopolysaccharides (LPS, 10 mg/kg, i.p.), and hearts were isolated 5-hrs or 16-hrs later. Isolated hearts with constant-pressure or constant-flow mode were examined by Langendorff technique. Rate and force of contractions of isolated atrial and ventricular strips were examined by tissue myography. Isolated endotoxemic hearts were characterized by decreased or increased coronary flow (CF) in LPS-treated-for-5hr and LPS-treated-for-16-hr groups, respectively, with decreased inotropy in both groups. Oro-A-perfusion ameliorated while AVP-perfusion worsened the decreased CF and inotropy in both preparations. Oro-A and AVP, however, did not affect diminished force or rate of contraction of atrial and ventricular strips of endotoxemic hearts. Oro-A-induced CF increase was not affected following coronary endothelium-denudation with saponin. These results suggest that Oro-A ameliorates LPS-depressed cardiac functions by increasing CF, leading to positive inotropy. In contrast, AVP aggravates cardiac dysfunction by decreasing CF. Oro-A is a potentially useful candidate for treating endotoxemia complicated with myocardial dysfunction.