Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Table 3

Primer sequences of the internal control gene and the target genes for PCR.

Genes	Primer sequence	Annealing temperature (°C)	Product length (bp)
U6	F: 5′GCTTCGGCAGCACATATACTAAAAT3′	60	89
	R: 5′CGCTTCACGAATTTGCGTGTCAT3′
rno-miR-129-5p	GSP: 5′GGAACTTTTTGCGGTCTGG3′	60	63
	R: 5′GTGCGTGTCGTGGAGTCG3′
rno-miR-322-5p	GSP: 5′GGGCAGCAGCAATTCAT3′	60	65
	R: 5′CAGTGCGTGTCGTGGAG3′
rno-miR-301a-3p	GSP: 5′CCCCGTGCAATAGTATTGT3′	60	65
	R: 5′CAGTGCGTGTCGTGGAGT3′

Primer sequences of the internal control gene and the target genes for PCR were listed in Table 3. GSP is the specific primer for the corresponding miRNA and R is the matching primer for the corresponding RT Primer.