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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 364519, 16 pages
http://dx.doi.org/10.1155/2013/364519
Research Article

Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury

1Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, 101 Kuang-Fu Road, Section 2, Hsinchu 30013, Taiwan
2Department of Applied Science, National Hsinchu University of Education, Hsinchu 30013, Taiwan
3Institute of Molecular Medicine and Department of Medical Science, National Tsing Hua University, Hsinchu 30013, Taiwan

Received 10 August 2012; Revised 22 November 2012; Accepted 7 February 2013

Academic Editor: Peng Nam Yeoh

Copyright © 2013 Yi-Wen Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Quercetin, a polyphenolic compound existing in many vegetables, fruits, has antiinflammatory, antiproliferation, and antioxidant effect on mammalian cells. Quercetin was evaluated for protecting cardiomyocytes from ischemia/reperfusion injury, but its protective mechanism remains unclear in the current study. The cardioprotective effects of quercetin are achieved by reducing the activity of Src kinase, signal transducer and activator of transcription 3 (STAT3), caspase 9, Bax, intracellular reactive oxygen species production, and inflammatory factor and inducible MnSOD expression. Fluorescence two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) can reveal the differentially expressed proteins of H9C2 cells treated with H2O2 or quercetin. Although 17 identified proteins were altered in H2O2-induced cells, these proteins such as alpha-soluble NSF attachment protein (α-SNAP), Ena/VASP-like protein (Evl), and isopentenyl-diphosphate delta-isomerase 1 (Idi-1) were reverted by pretreatment with quercetin, which correlates with kinase activation, DNA repair, lipid, and protein metabolism. Quercetin dephosphorylates Src kinase in H2O2-induced H9C2 cells and likely blocks the H2O2-induced inflammatory response through STAT3 kinase modulation. This probably contributes to prevent ischemia/reperfusion injury in cardiomyocytes.