Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study

Figure 6

Effect of additives on the thermal unfolding of xylanase followed by DSC. (a) The thermogram of xylanase (1 mg·mL⁻¹) was obtained at 15°C·h⁻¹ scan rate in the presence of 0.4 M GL. (b) The thermogram of 1 mg·mL⁻¹ protein was obtained in the presence of 0.5% (w/v) soluble beechwood xylan at 15°C·h⁻¹ scan rate. Dotted and coloured solid lines in (a) and (b) mean the same as in Figure 5(a). (c) Irreversible calorimetric transition of 0.4 mg·mL⁻¹ xylanase containing 1.5 M sorbitol at 60°C·h⁻¹ scan rate. Inset: linear dependence of the apparent on sorbitol concentration (0.5–2 M).

(a)

(b)

(c)