Insight into Glutamate Excitotoxicity from Synaptic Zinc Homeostasis
Figure 1
Changes in zinc signals in the extracellular and intracellular compartments in the hippocampal CA1 during tetanic stimulation. (a) Hippocampal illustration and CA1 image with ZnAF-2. (b) High-frequency stimulation (HSF, 200 Hz, 1 s) was delivered to the Schaffer collaterals in hippocampal slices stained with ZnAF-2 or ZnAF-2DA. The circle (around 10 μm in diameter) shown in Figure 1(a) is a representative example of the region of interest. The data represents the changed rate (%) in fluorescent signals to the basal fluorescent signal before the stimulation, which is expressed as 100%. The red bar indicates the period of electrical stimulation. (c) Tetanic stimulation (200 Hz, 1 s) was delivered to the Schaffer collaterals in hippocampal slices immersed in ACSF (control), 1 mM CaEDTA in ACSF, or 10 μM CNQX in ACSF. The data represents the changed rate (%) in fluorescent signal during tetanic stimulation to the basal fluorescent signal before the stimulation, which is expressed as 100%. ***, versus the basal level before the stimulation; ##, ###, versus the control (stimulated in ACSF). This data is cited from the paper published by Journal of Neuroscience Research, 2007 [35].
