Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells
Figure 4
Quantitative changes in glutathione thiol-disulfide redox state during B cell differentiation. Values from experiments performed on three independent cultures are given as mean ± SEM. The values for resting B cells are represented as “Day 0.” All data are normalized to the amino acid content of the TCA precipitated cells and data processing is performed as described in the legend of Table 1. (a) SH/aa values of intracellular GSH (white bars) and (GS in GSSG) (black bars). (b) Percent (GS in GSSG) relative to (Total GS). (c) Percent (GS in PSSG) relative to (Total GS). (d) SH/aa values of GS equivalents quantified from the media.
