Methylation of the Corticotropin Releasing Hormone Gene Promoter in BeWo Cells: Relationship to Gene Activity

Figure 5

CpG methylation in individual copies of the CRH proximal promoter. Three replicate sets of BeWo cells were processed for clonal bisulfite sequencing at the 0 h (upper panel) and 72 h (lower panel) treatment times. Ten randomly selected clones were sequenced from each culture. Each line represents one copy of the promoter with open and closed circles denoting unmethylated and methylated CpGs, respectively. CpG site numbers as shown in Figure 3 are also indicated.