UHPLC/MS-MS Analysis of Six Neonicotinoids in Honey by Modified QuEChERS: Method Development, Validation, and Uncertainty Measurement

Table 3

Results of individual and combined uncertainties for each pesticide calculated at 10 μg/kg concentration level.

Compound	Standard solution	Weighting	Calibration curve	Precision	Dilution operations	Combined uncertainty	Expanded uncertainty	Relative expanded uncertainty
	𝑈1	𝑈2	𝑈3	𝑈4	𝑈5	𝑈	2𝑈	𝑈%
Thiamethoxam	0.003	0.00001	0.064	0.044	0.070	1.1	2.2	21
Nitenpyram	0.003	0.00001	0.164	0.082	0.070	1.5	3.0	39
Imidacloprid	0.003	0.00001	0.161	0.011	0.070	2.0	4.0	35
Clothianidin	0.003	0.00001	0.089	0.033	0.070	1.3	2.6	23
Acetamiprid	0.003	0.00001	0.232	0.019	0.070	2.6	5.2	49
Thiacloprid	0.003	0.00001	0.220	0.024	0.070	2.1	4.2	46