Effect of Chronic Consumption of Sweeteners on Microbiota and Immunity in the Small Intestine of Young Mice

Table 6

(a) Microbial species identified in the small intestine of CD1 mice without treatment (21 days of age) and after 6 weeks of treatment with sweeteners.

Group	Number of Identified strains	Key of strains	Fragment size (bp)	% of Similarity in BLAST	Number Access to GenBank	Identified species
Basal	6	Bsl-1	1527	99	NR_075803	Rothia dentocariosa strain ATCC 17931
		Bsl-2	1398	99	NR_075803	Rothia dentocariosa strain ATCC 17931
		Bsl-3s	1404	99	AB33945	Bacillus pumilus strain NBRC 12092
		Bsl-4s	1397	98	AB661448	Acinetobacter haemolyticus strain ATCC 17906
		Bsl-5As	1401	98	AB661448	Acinetobacter haemolyticus strain ATCC 17906
		Bsl-6s	1412	99	NR_025412	Acinetobacter schindleri strain LUH 5832
Control A	4	ClA-1	1394	99	NR_025228	Pseudomonas koreensis strain Ps 9-14
		ClA-1s	1399	99	AB661448	Acinetobacter haemolyticus strain ATCC 17906
		ClA-2	1448	98	NR_113350	Staphylococcus xylosus strain JCM 2418
		ClA-3s	1418	97	NR_113350	Staphylococcus xylosus strain JCM 2418
Sucrose A	10	SacA-2	1397	97	M87887	Bacillus subtilis strain 168
		SacA-2s	1401	99	NR_113405	Staphylococcus saccharolyticus strain JCM 1768
		SacA-3	1404	99	NR_042147	Pseudomonas cedrina subsp fulgida strain P515/12
		SacA-5	1385	98	AB681259	Bacillus safensis strain NBRC 100820
		SacA-6	1400	99	AB680322	Pseudomonas azotoformans strain NBRC 12693
		SacA-7s	1406	99	NR_042338	Bacillus aerius strain 24K
		SacA-8s	1387	99	NR_112569	Lysinibacillus fusiformis strain NBRC 15717
		SacA-9s	1416	98	NR_121761	Bacillus toyonensis BCT 7112
		SacA-10s	1410	99	NR_113350	Staphylococcus xylosus strain JCM 2418
		SacA-12s	1393	99	NR_028930	Stenotrophomonas rhizophila strain e-p 10
Splenda® A	8	SucA-1A	1399	99	NR_024817	Bacillus asahii strain MA001
		SucA-1s	1403	99	NR_025361	Arthrobacter albus strain CF-43
		SucA-2s	1404	99	NR_025723	Kocuria marina strain KMM 3905
		SucA-3	1399	100	NR_108906	Bacillus eiseniae strain A 1-2
		SucA-3s	1398	100	NR_116578	Micrococcus yunnanensis strain YIM 65004
		SucA-5	1376	98	FJ357586	Staphylococcus epidermidis strain BBN 1B3-02
		SucA-6s	1399	97	KY818992	Pseudomonas knackmussii strain FL 80
		SucA-7s	1415	98	NR_112723	Bacillus atrophaeus strain NBRC 15539
Svetia® A	8	StA-1	1408	99	AB681259	Bacillus safensis strain NBRC 100820
		StA -2	1409	100	NR_113350	Staphylococcus xylosus strain JCM 2418
		StA -1s	1409	98	NR_042338	Bacillus aerius strain 24K
		StA -2A	1403	99	NR_113350	Staphylococcus xylosus strain JCM 2418
		StA -2s	1409	98	KY174334	Bacillus licheniformis strain DSM 13
		StA -3	1397	98	NR_112632	Bacillus circulans strain NBRC 13626
		StA -3s	1404	99	NR_112723	Bacillus atrophaeus strain NBRC 15539
		StA -5	1407	98	NR_126178	Streptococcus saliviloxodontae strain NUM 6306
Total	36

(b) Microbial species identified in the small intestine of CD1 mice of 12 weeks of treatment with sweeteners.

Group	Number of identified strains	Key of strains	Fragment size (bp)	% of Similarity in BLAST	Number access in GenBank	Identified Strains
Control B	6	CtB-1	1401	97	NR_075022	Enterococcus hirae strain ATCC 9790
		CtB-2	1408	98	NR_104284	Bacillus muralis strain LMG 20238
		CtB-2s	1393	98	AB33945	Bacillus pumilus strain NBRC 12092
		CtB-3	1398	99	NR_042161	Bacillus ruris strain R-6760
		CtB-3s	1395	98	NR_118146	Lysinibacillus mangiferihumi strain M-GX 18
		CtB-4s	1398	99	NR_043314	Pseudomonas moraviensis strain 1B4
Sucrose B	12	SacB-2s	1400	99	NR_116578	Micrococcus yunnanensis strain YIM 65004
		SacB-2	1405	97	GU252112	Bacillus megaterium strain ATCC 14581
		SacB-3	1396	99	NR_074923	Bacillus licheniformis strain ATCC 14580
		SacB-3s	1395	100	NR_121761	Bacillus toyonensis strain BCT-7112
		SacB-4	1407	99	NR_114270	Rummeliibacillus stabekisii strain NBRC 104870
		SacB-4s	1396	98	NR_075022	Enterococcus hirae strain ATCC9790
		SacB-6s	1393	98	NR_075022	Enterococcus hirae strain ATCC9790
		SacB-7	1410	100	NR_117562	Enterococcus lactis strain BT 159
		SacB-8	1406	99	NR_118381	Bacillus pumilus strain SBMP2
		SacB-9	1396	99	KT719422	Rummeliibacillus stabekisii strain MER_TA_13
		SacB-9s	1407	99	NR_121761	Bacillus toyonensis strain BCT-7112
		SacB-10	1409	99	NR_114270	Rummeliibacillus stabekisii strain NBRC 104870
Splenda® B	3	SucB-1s	1403	99	KY992565	Staphylococcus xylosus strain 2B
		SucB-3s	1406	99	KY316471	Bacillus cereus strain ZLynn1000-57
		SucB-4	1408	99	MF040255	Bacillus pumilus strain LMB3G14
Svetia® B	3	StB-1s	1415	99	NR_024668	Staphylococcus lugdunensis strain ATCC43809
		StB -4s	1415	99	NR_112845	Oceanobacillus sojae strain Y27
		StB -6	1402	100	AB681259	Bacillus safensis strain NBRC 100820
Total	24