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International Journal of Genomics
Volume 2013 (2013), Article ID 972852, 19 pages
Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage
1Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Beidi Road 2901, Minhang District, Shanghai 201106, China
2Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China
3College of Fishery and Life Science, Shanghai Ocean University, Shanghai 201306, China
Received 7 August 2012; Revised 31 March 2013; Accepted 3 April 2013
Academic Editor: Margarita Hadzopoulou-Cladaras
Copyright © 2013 Runhong Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Salinity is one of the major abiotic stresses that affect crop productivity. Identification of the potential novel genes responsible for salt tolerance in barley will contribute to understanding the molecular mechanism of barley responses to salt stress. We compared changes in transcriptome between Hua 11 (a salt-tolerant genotype) and Hua 30 (a salt sensitive genotype) in response to salt stress at the seedling stage using barley cDNA microarrays. In total, 557 and 247 salt-responsive genes were expressed exclusively in the shoot and root tissue of the salt-tolerant genotype, respectively. Among these genes, a number of signal-related genes, transcription factors and compatible solutes were identified and some of these genes were carefully discussed. Notably, a LysM RLK was firstly found involved in salt stress response. Moreover, key enzymes in the pathways of jasmonic acid biosynthesis, lipid metabolism and indole-3-acetic acid homeostasis were specifically affected by salt stress in salt tolerance genotype. These salt-responsive genes and biochemical pathways identified in this study could provide further information for understanding the mechanisms of salt tolerance in barley.
Due to various biotic and abiotic stress factors under field conditions, crop plant yield reduction can reach more than 50% . Among these abiotic stresses, salinity is the most severe environmental stress affecting more than 800 million hectares of land throughout the world [2, 3]. Unsuitable irrigation was the most significant reason leading to cultivated agricultural land salinization . With the constantly growing world population, the demands for food are increasing rapidly, so it is an important global priority to improve the salt tolerance of crops . The discovery of novel genes, the analysis of their expression patterns in response to salt stress, and the determination of their potential functions in salt stress adaptation will provide the basis of effective engineering strategies to enhance crop salt stress tolerance .
To cope with the detrimental effects of various abiotic stresses, crops have evolved many mechanisms to increase their tolerance, including physical adaptations, and interactive molecular and cellular changes . The crops can switch on these mechanisms through a signal transduction pathway when they perceive environmental stress [7, 8]. Understanding the mechanisms of signal transduction is not only of fundamental importance to biology but also essential for the continued development of rational breeding and transgenic strategies to improve stress tolerance in crops .
During recent years, considerable attention has been directed toward elucidating the molecular basis of plant salt tolerance. Several important pathways involved in salt stress signal transduction have been identified from Arabidopsis and rice, such as the salt oversensitive (SOS) pathway [9, 10], the calcium-dependent protein kinase (CDPK) pathway , and the mitogen activated protein kinase (MAPK) pathway , the oxylipin pathway , and endoplasmic reticulum stress signaling . Also, plant hormones, such as abscisic acid (ABA), ethylene, salicylic acid, and jasmonic acid, all play vital roles in salt-stress signaling and adaptation [15–17]. Considerable research has shown that different pathways are interconnected and coordinately regulate the plant response to biotic and abiotic stresses [6, 16, 18–20].
Barley (Hordeum vulgare L.) is an important crop usually used as human food, malt, and feed for animals  and is the most important field crop after rice, wheat and maize . Among the cereal crops, barley is considered as a notably salt-tolerant cultivar [23, 24], which also shows considerable variation for tolerance towards salinity stress [3, 25]. Furthermore, the salt tolerance of barley varies with the plant growth stage. It is reported that the germination and young seedling stage is the most sensitive and affects the final yield. However, the barley exhibits an increased tolerance with age . Investigating the salt-tolerance mechanisms in barley could facilitate a better understanding of the genetic basis of salt tolerance and therefore enable the effective use of genetic and genomic approaches to improve salt tolerance.
Transcriptome analysis has always played a central role in elucidating the complexity of gene expression regulation. Among several transcriptome analysis methods, microarray technologies and RNA-seq have become the default popular methods of choices for genomewide transcriptome studies . Although RNA-seq has recently become a preferred method of choice in whole transcriptome analysis, microarrays represent a well-established technology and have been widely used in the last decades and have provided a great deal of candidate genes for genetic engineering [24, 27, 28]. In the current study, a cDNA microarray technology was used to identify salt stress-regulated genes on a large scale and to clarify the complex molecular mechanisms of barley salt tolerance from two cultivated barley genotypes: Hua 30, a salt-sensitive cultivar, and Hua 11, a salt-tolerant cultivar. These cultivars were derived from microspores of progeny of the same parental cross and display highly similar phenotypes in developmental and responsive processes other than salt tolerance. Our previous work has disclosed that Hua 30 and Hua 11 showed evident difference in salt tolerance at distinct developmental stages [29–31]. Thus they provide excellent materials for barley salt tolerance research. According to gene expression patterns, the salt stress responsive genes can be broadly classified as early- and late-induced genes . It is reported that the early responsive genes are important to environmental stress response [8, 32]. Previous studies have provided well understanding of transcription responses of barley to salinity stress after long time exposure, for example, 24 h [33, 34], 27 h , and 5 days . However, few reports were available on the early responses . To bridge the gaps, transcriptional analysis was performed at 6 h after salt treatment in two genotypes showing different tolerance to salinity.
2. Materials and Methods
2.1. Plant Cultivation and Salt Stress Treatment
Seeds of the salt-tolerant cultivar, Hua11, and the salt-sensitive cultivar, Hua30, were obtained from the Biotechnology Research Center of the Shanghai Academy of Agricultural Sciences. Uniformly sized seeds were selected, surface-sterilized in 75% ethanol for 1 min and rinsed several times, germinated in distilled water overnight, and then placed on moist filter paper of deep cell culture dishes in a growth chamber. After 7 d, seedlings at similar germination stage were suspended in a half-strength Hoagland solution with double iron (50 g·L−1). The pH of the solution was maintained within the range of 5–6.5 using KOH. Plants were grown in controlled conditions at 25/22°C day/night, 12 h photoperiod, and 70% humidity. The salt stress was imposed from the 11th day after germination. CaCl2 was added with NaCl to maintain an Na+/Ca2+ concentration ratio of 10 : 1 on a molar basis. In the preexperiment, 150, 200, 250, 300, and 400 mM NaCl gradient stress was applied on barley seedlings for three days to judge the optimum concentration for salt stress. According to the preexperiment results, 300 mM was chosen as our stress scale due to the more phenotypic changes observed between Hua 30 and Hua 11 than others. As mentioned before, on 11th day old seedlings were transferred either to half-strength Hoagland with 300 mM NaCl for salt stress treatment or to half-strength Hoagland solution without salt as the control. Roots and shoots from 15 plants were harvested separately from control and salt-stressed at 6 h after the treatment, frozen in liquid nitrogen, and then kept at −70°C for RNA extraction.
2.2. Physiological Assays
According to the previous method, roots and shoots were harvested separately at 0 h, 6 h, 48 h, and 72 h after salt-stressed for the POD (peroxidase) and SOD (superoxide dismutase) activities determination.
The activity of POD was assayed by the method of Evans and Alldridge  with lesser modifications. The reaction mixture was treated in water bath at 37°C for 5 min and cooled in an ice bath. The total peroxidase activity was expressed as the increase in absorbance at 470 nm min−1 g−1 FW (0.01 OD = 1 enzyme unit).
The activity of SOD was assayed by the method of Droillard et al.  with measuring its ability to inhibit the reduction of nitroblue tetrazolium (NBT). The absorbance was measured at 560 nm of the reaction mixture. The volume of enzyme extract corresponding to 50% inhibition of the reaction was regarded as 1 enzyme unit.
2.3. RNA Preparations and Gene Chip Hybridization
Total RNA was extracted using the TRIzol reagent (Invitrogen, USA) according to the manufacturer’s instructions. The quality of RNA was analyzed on a denaturing formaldehyde gel and confirmed by measuring the ratio of A260/A280 with the NanoDropND-1000 spectrophotometer.
Sample treatment, hybridization, and scanning of Affymetrix Barley 1 GeneChip with 22792 probe sets were carried out at the College of Life Sciences, Zhejiang University, as described in reference of Cheng et al. . The SuperScript double-stranded cDNA synthesis kit (Invitrogen) was used to synthesize complementary DNA. A part of the double-stranded cDNA was used as a template to produce biotin-tagged cRNA by an Affymetrix GeneChip IVT labeling kit (Affymetrix). Fifteen micrograms of the biotin-tagged cRNA was separated into a size range of 35 to 200 bases following the manufacturer’s instructions. Then 10 ug of this fragmented biotin-tagged cRNA was hybridized at 45°C with rotating for 16 hr to probe sets present on GeneChip Drosophila genome array. These genome arrays were washed and stained using streptavidin-phycoerythrin on an Affymetrix Fluidics Station 400, as described in the Affymetrix protocols, and then were scanned using the Hewlett-Packard GeneArray Scanner G3000. The hybridization data were analyzed using Microarray Suite version 5.0. Two biological replicates per treatment and per genotype were analyzed.
2.4. Statistical Analysis of the Microarray Data
Raw intensity data were firstly normalized using the MAS5 algorithm, which allowed probe identifier present calls to be determined. Only those probe sets which called present in two replicates under treatment were included and then probe intensities were analyzed by the GC-RMA algorithm and log transform was carried out. Subsequently, the average log signal intensity values of two biological replicates for each sample were calculated and used for further analysis. Genes that had significant expression between the control and salt-treated roots or shoots of each variety were identified at with an empirical Bayes’ -test using false discovery rate (FDR) for multiple testing corrections  and by using the empirical criterion of greater than a twofold change.
2.5. Probe Set Annotations, Gene Ontology Analysis, and Biochemical Pathways Analysis
The probe sets were annotated using HarvEST:Barley (version 1.77) assembly 21(HarvEST:Barley (http://harvest.ucr.edu/)), which can provide the best BLASTX hit against UniProt, TIGR rice, and TAIR Arabidopsis database with a cut-off threshold of E-20. The least number of probes matched selected for the annotation was 11. Blast2go (http://www.blast2go.com/b2glaunch) was used to analyze gene ontology and then KOBAS (KEGG-Ontology (KO-) Based Annotation System) was used to identify the most statistically significantly enriched KEGG pathways and the Arabidopsis thaliana genome was used as the background distribution.
2.6. Quantitative Real-Time PCR
The expression profiles of several important transcripts obtained from gene chip hybridizations were further validated by real-time PCR using the first strand cDNA synthesis from independently isolated RNA samples. A cDNA first strand was synthesized using M-MLV first strand kit (Invitrogen, cat no. C28025-032) following the manufacturer’s instructions. Five micrograms of total RNA was converted into 40 μL cDNA. Each cDNA was diluted 20 times and 2 μL of cDNA was used for two-step PCR. PCR was performed with the SYBR Green Real-time PCR Master Mix (TOYOBO, code no. QPK 201) in a reaction volume of 20 μL containing 2 μL of diluted cDNA. Cycling was carried out on an Applied Biosystems StepOne Real-Time PCR System following the manufacturer’s instructions. The ten genes randomly selected for validation only included those that had upregulated expression in response to salinity stress. Seven genes belonged to the shoot and the others belonged to the root. All PCRs were repeated three times for biological replicates at each sampling time point. A barley actin gene (forward primer: GCCGTGCTTTCCCTCTATG; reverse primer: GCTTCTCCTTGATG TCCCTTA) was used as a control for real-time PCR.
3.1. Response of Hua 30 and Hua 11 at Seedling Stage to Salinity Stress
Salt tolerance in barley varieties of Hua 30 and Hua 11 was identified clearly at the microspore stage , germination stage , and seedling stage . In this experiment, no significant difference at the seedling stage was observed between the two genotypes under normal conditions (Figure 1(a)), while under severe salt stress (300 Mm NaCl), phenotypic changes were observed in Hua 30 compared with Hua 11: first leaves apex turned yellow (Figure 1(c)), reduced growth (Figure 1(c)), and the severe dehydration of new leaves (Figure 1(d)).
3.2. Effect of Salt Stress on POD and SOD Activities in Barley
Salinity, like other environment stresses, also triggers plant to generate reactive oxygen species (ROS) . However, stress-induced ROS accumulation is counteracted by enzymatic antioxidant systems or nonenzymatic low molecular metabolites . Superoxide dismutase (SOD; EC 220.127.116.11) is a major scavenger of superoxide (O2−), and the products of its enzymatic action are H2O2 and O2. Subsequently the hydrogen peroxide produced is scavenged by a variety of peroxidases (POD: EC 18.104.22.168). Therefore, the activities of SOD and POD play important roles in the protection of barley plants from salt stress
Before treatment, the background level of shoot SOD activity was high in Hua 11 than that in Hua 30 (Figure 2(a)). Salt treatment had no significant impact on SOD activity in the shoot tissue of Hua 30, whereas SOD activity in shoot tissue of Hua 11 was decreased slightly and then increased obviously. Similarly, the background level of root SOD activity was high in Hua 11 than that in Hua 30 before salt treatment. During 72 h salt treatment, similar fluctuation in SOD activity was observed in Hua 11 and Hua 30 roots, but the SOD activity always was much higher in Hua 11 root than in Hua 30 root.
Before salt treatment, the POD activity was a little higher in root but nearly similar in shoot of Hua11 as compared with that in Hua 30 corresponding tissues (Figure 2(b)). It rose steadily after salt treatment in both shoot and root tissues of Hua 11 while it was fluctuated in both tissues of Hua 30. It was particularly higher in Hua 11 root than in Hua 30 root after 72 h salt.
In conclusion, the SOD and POD activities in Hua 11 were higher than Hua 30 in both shoots and roots, which provides sound biochemical basis for the higher tolerance of Hua 11 to salt stress than Hua 30.
3.3. Expression Profiles of Salt-Responsive Genes in Barley
Changes of gene expression in Hua 11 and Hua30 under salt stress were investigated using Barley 1 microarrays. A total of 1853 and 1473 differentially regulated probe sets were found in the shoot tissue and root tissue, respectively, of the two genotypes after 6 h of salt treatment (Figure 3). In root tissue, the number of differentially regulated probe sets in Hua 11 and Hua 30 was 1163 and 1226. By contrast, 916 coregulated probe sets being identified in two genotypes, with only 247 probe sets were identified in Hua 11 and 310 probe sets in Hua 30. In the shoot tissue, the number of salt responsive probe sets in Hua 11 and Hua 30 was 1399 and 1296. By comparison, 842 coregulated probe sets being found in two genotypes, with only 557 probe sets were found in Hua 11 and 454 probe sets in Hua 30 (Table 1).
These significantly changed probe sets related to signal transduction, transcription factors, and compatible and secondary metabolites were classified (Table 1). The number of differentially regulated probe sets related to signal transduction was 176 and 103 in shoot and root tissues, respectively, which only in Hua 11 was 59 and 19 while only in Hua 30 was 38 and 21. For the salt responsive transcription factors, 91 and 49 probe sets were found in shoot and root tissue, which only in Hua 11 was 30 and 6 while only in Hua 30 was 16 and 10. Regard to the compatible and secondary metabolites, there were 32 and 33 different expression probe sets were found in shoot and root tissue, which only in Hua 11 was 25 and 11 while only in Hua 30 was 13 and 10.
3.3.1. Signal Transduction Elements Responsive to Salt Stress in the Salt-Tolerant Genotype
It was suggested that genes that showed a significant response to salt in the salt-tolerant genotype Hua 11, but did not respond (un-altered or even reversely altered) in the salt-sensitive genotype Hua 30 are likely to be related to salt tolerance . To investigate the signal transduction process in barley under salt stress conditions, differentially-expressed signaling-related genes were subjected to analysis. 59 genes in shoot tissue and 18 genes in root tissue involved in signaling pathways were identified in the salt-tolerant genotype Hua 11 (Table 2). These genes were classified into four major groups including kinases, phosphatases, hormone-related genes and others.
The first group is kinases, and contained receptor-like kinases (RLKs), Ser/Thr kinases, CIPK, CDPK, MAPK and others kinases, with 14 RLKs identified as salt stress-responsive genes in the Hua 11 shoot tissue and 0 in the root tissue. All of these RLKs were upregulated by salt treatment. 10 Ser/Thr kinases (9 up and 1 down) in shoot tissue and 4 (3 up and 1 down) in root tissue were identified as salt-responsive genes in Hua 11. In addition, two CDPKs (calcium-dependent protein kinase) and one MAPK (mitogen-activated protein kinase) were identified in shoot tissue, and one CIPK was identified in root tissue. The remaining kinases include two ribose-phosphate pyrophosphokinases (Contig5081_at and Contig8025_at), one amino acid kinase (Contig12389_s_at), one phosphatidylinositol-4-phosphate 5-kinase (Contig19429_at), one phosphatidylinositol kinase and FAT-containing domain protein (Contig8975_at), one protein kinase domain-containing protein (Contig11835_at), one unknown function DUF1296 domain-containing protein (Contig5785_at), one tyrosine protein kinase domain-containing protein (Contig19683_at) in shoots, and one phosphomethylpyrimidine kinase (Contig15880_at) in root tissue. These genes were upregulated in Hua 11.
The second group is phosphatases. They were divided into two major classes: protein serine/threonine phosphatases and protein tyrosine phosphatases. 7 serine/threonine phosphatases were identified in shoot tissue (6 up and 1 down) and one up-regulated gene was identified in root tissue. Only one protein tyrosine phosphatase (Contig9830_s_at) was identified in shoot tissue.
The third group is hormone-related genes, which included jasmonic acid (JA), ethylene, gibberellins, auxin, and cytokinin. Most of these genes were up-regulated.
The remaining signal-related genes were second messengers and response regulators. Only one of the 7 remaining signal-related genes was downregulated in the shoot tissue, and one gene out of 2 was downregulated in the root tissue.
3.3.2. Transcriptional Regulation Responsive to Salt in the Salt-Tolerant Genotype
To understand the transcriptional regulation in salt stress response in the salt-tolerant genotype Hua 11, 30 genes identified in the shoot and 6 genes identified in the root of Hua 11, all of which encoding transcription factors, were further characterized (Table 3). These results suggest the existence of many transcriptional regulatory mechanisms in high-salinity stress signal transduction pathways. Among these salt stress-inducible and Hua 11-specific transcription factors in shoot tissue, there are two ZIM motif family genes, nine Zinc finger family genes, four WRKY family genes, three MYB family genes, two basic helix-loop-helix (bHLH) family genes, two C-repeat binding factors, two NAC family genes, two basic leucine zipper (bZIP) family genes, three AP2 family genes, and one whirly family gene. Most of these were up-regulated, with the exception of four genes. Six salt responsive genes coding transcription factors were found Hua 11-specific in root tissue, including two Zinc finger family genes, one WRKY family gene, one MYB family gene, one bZIP family gene, and one Homeobox-leucine zipper gene. Only two genes (one MYB family gene, one bZIP family gene) were down-regulated in root.
3.3.3. Metabolism Responsive to Salt in the Salt-Tolerant Genotype
The major roles of compatible solutes and plant secondary metabolites are to protect plants in order to survive biotic and abiotic stresses. In order to assess the compatible solutes and secondary metabolites in salt stress response in the salt-tolerant genotypes, 15 genes in shoots and 11 genes in roots were identified in the tolerant genotype Hua11 (Table 4). The compatible solutes are all up-regulated in shoots and roots. One proline-related gene and 4 sugar metabolite-related genes were found in shoot tissue, and one sugar metabolite related gene was found in root tissue. The secondary metabolite genes have a simple classification that includes three main groups. The first group is phendic: one flavonoid-related gene (Contig11944_at), two simple phenolic genes (Contig24054_at; Contig24228_at), and one quinine-related gene (Contig12883_at) were identified in the shoot tissue; three flavonoid-related genes (Contig25479_at; Contig17030_at; Contig22018_at) and three simple phenolic-related genes (Contig8345_at; Contig3308_at; Contig6733_at) were identified in the root tissue. The second group is terpenoid: only two genes (HT09C21r_s_at; Contig3183_at) were identified from shoot tissue. The third group is nitrogen-containing compounds: three alkaloids-related genes (Contig13038_at; Contig13228_at; Contig14427_at) in shoot tissue and two genes (Contig2900_at; Contig23347_at) in root tissue were identified. Three amine-related genes (Contig5994_s_at; Contig13254_at; Contig6067_at) were also identified from root tissue.
3.3.4. Gene Ontology Analysis of the Salt Stress Responsive Genes
To investigate the identity of the differentially expressed genes, these genes were annotated by the HarvEST software version 1.77 based on the best BLAST matches to UniProt, Arabidopsis, and rice gene sequence. As a result, most of the differentially expressed genes could be assigned to a best scoring BLAST hit and more than 70% genes had the GO assignments. Using blast2go software, these differentially expressed genes with the GO assignments were classified into different GO groups including molecular function, biological process, and cellular component (Table 5 and Figure 4).
Regarding biological processes, genes involved in cellular process, metabolic process, cellular process, and response to stimulus were highly represented. The well-represented molecular functions were binding and catalytic activity. For cellular component, the most represented categories were cells, organelles, and membrane (Figure 4).
3.3.5. Biochemical Pathways Affected by Salt Stress in the Salt-Tolerant Genotype
A lot of differentially expressed genes were isolated in our study. In order to assess the functional roles of these salt-responsive genes involved in biochemical pathways, specific biochemical metabolisms affected by salt stress only in the salt-tolerant genotype were identified by KO-Based Annotation System (KOBAS), which can provide the most frequent and statistically significantly enriched pathways. In the shoot tissue of Hua 11, 13 pathways were identified from the up-regulated genes with the value <0.05, including 38 genes. Meanwhile, 6 pathways were identified in the root tissues with the value <0.05; 7 genes were involved (Table 6). These pathways came from Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway databases and BioCyc databases. In the shoot tissue, the pathways about PRPP biosynthesis I, jasmonic acid biosynthesis, 13-LOX and 13-HPL pathway, very long chain fatty acid biosynthesis and sucrose biosynthesis were classified into biosynthesis in BioCyc databases. All others came from KEGG pathway databases which can be classified into three categories. The first one was metabolism containing N-glycan biosynthesis, linoleic acid metabolism, and sphingolipid metabolism. The second category was genetic information processing and the protein processing in endoplasmic reticulum, ribosome biogenesis in eukaryotes, RNA transport, and spliceosome which were included. The plant-pathogen interaction belonging to organismal systems was the last category. In the root tissue, all the pathways came from the BioCyc databases except the lipid metabolism which was derived from KEGG pathway databases and belonged to the category of metabolism.
3.4. Validations of Microarray Results by Real-Time PCR
To confirm the microarray results, real-time PCR was conducted on 10 randomly selected salt-responsive genes based on the microarray (Table 7). Seven genes belonged to those differentially expressed in shoot and three genes belonged to those in root. The real-time PCR analysis showed that only result of one gene (Contig5740_at) did not agree with the microarray data, which indicated that there was a good consistency between the real-time PCR and microarray results (Figure 5).
Using the transcriptional profiling of Barley1 chip, it found that the salt tolerant barley: Hua 11 and salt sensitive barley: Hua 30 exhibited a diverse transcriptional response under the salt stress. Interestingly, comparison of the expression levels of gene between Hua 11 and Hua 30 revealed that 916 (62%) genes and 842 (45%) genes were co-regulated, respectively in root and shoot tissue. These genes might be responsible for barley intrinsic tolerance to salt stress. The most attention is that the number of salt responsive genes identified from shoot tissues (1853) more than from root tissues (1475). The similar results had got in rice [41, 42], which demonstrates the tissue-specific nature of the salinity response not only in rice but also in barley. Mangelsen et al.  reported that the early heat stress respond in barley was divide into three distinct temporal phases termed sensing and signal transduction, primary heat stress response, and heat stress adaptation. Although the salt stress is differing from the heat stress, the similar mechanism will be shared among the abiotic stresses. A reasonable explain for this phenomenon may be the salt responsive gene from root mostly in the phase of adaptation while the genes identified from shoot were involved in the first two phases after 6 h of salt stress. The salt-responsive genes only expressed in the salt tolerant genotype might participate in the special process of salt tolerance in barley. Comparison of gene expression profiles between the salt tolerant genotype and salt sensitive genotype under salt stress is essential for the elucidation of the salt response networks in barley.
4.1. Signal Transduction and Hormonal Regulation under Salt Stress in Barley
Stress signal transduction plays a crucial role in plant stress response . Kinases and phosphates can turn on or off salt stress responses by reversible phosphorylation to activate transcription factors and other functional genes [32, 44]. In this study, a large number of signaling-related elements were identified.
Receptor-like kinases (RLKs) play vital roles in plant development, hormone perception, and biotic or abiotic stress responses, which belong to the group of earliest response genes in plants under stress conditions . The architecture of receptor-like protein kinases (RLKs) indicates that RLKs can perceive and transmit external signals from the environment and then activate downstream signaling cascades to induce appropriate cellular responses [46, 47]. The RLKs have been shown to be highly induced by salt in Arabidopsis and rice [45, 48]. Among these RLKs, a somatic embryogenesis receptor kinase 1 (SERK 1) up-regulated in shoot tissue of Hua 11 was involved in the brassinosteroid (BR) signaling pathway, which led to a broader range of functions in plant responses to biotic and abiotic stimuli [49, 50]. A WAK receptor-like cytoplasmic kinase, as a signaling linker between the cell wall and the cytoplasm, was involved in biotic and abiotic stress responses [51, 52]. Interestingly, a WAK-like kinase in Arabidopsis, WAKL4, has been reported that it was significantly up-regulated by salt stress. This result was similar to our microarray data, Contig12629_s_at, a WAK receptor-like cytoplasmic kinase also up-regulated after salt stress . The LysM receptor-like kinase (LysM RLK) performs a critical function in chitin signaling and fungal resistance [46, 54]. However, an LysM RLK was remarkably induced by salt stress in this study and it was firstly found involved in salt stress response. This result may indicate that the LysM receptor-like kinase plays key roles in the crosstalk between biotic and abiotic stress signaling.
MAPKs involved in MAPK pathways are activated by diverse abiotic stresses and act as a link between upstream receptors and downstream targets [55, 56]. Ca2+ is implicated as a “second messenger” in signaling in responses to both biotic and abiotic stress [57–59]. Calcium-dependent protein kinases (CDPKs) can bind and sense calcium ions directly to induce the downstream signaling in response to different stresses . The CBL-CIPK signaling system, a newly emerging plant-specific and Ca2+-dependent network, mediates abiotic stress tolerance, for substances such as salt . In this study, MAPK and CDPK signaling pathways were up-regulated under salt stress, which were similar to the result of Zhang et al. . Calcium sensors and their interactive proteins were also identified in salt-tolerant genotype of Hua 11. These results indicated that MAPK pathway and Ca2+-dependent signaling pathway might play important roles in early response of barley under severe salt stress.
Protein phosphatases, by opposing the functions of the protein kinases, also have a general mechanism for transmitting signals from the extracellular surroundings to the interior of the cell . PP2B phosphatase calcineurin (CaN), a key component of Ca2+-dependent signal transduction pathway, functions to restrict intracellular Na+ accumulation to mediate salt adaptation in plants . PP2Cs not only act as negative regulators involved in the ABA signal transduction pathway, but also function as regulators of mitogen-activated protein kinase pathway and receptor kinase signaling , while these pathways all play important roles in stress signaling. The protein tyrosine phosphatases were another type of phosphatases which also are involved in the mitogen-activated protein kinases pathway . It has been reported that a tyrosine-specific protein phosphatase (AtPTP1) is positively regulated by high-salt exposure in Arabidopsis, which was similar to our result . In a word, all of these suggested that protein phosphatases may have crucial roles in NaCl stress signaling.
Phytohormones, such as abscisic acid (ABA), jasmonic acid (JA), and ethylene (ET), regulate the protective responses of plants against abiotic stresses by synergistic and antagonistic actions . In this study, a set of salt-regulated hormone-related proteins were identified both in shoot and root tissues in Hua 11. Among them, three lipoxygenases were up-regulated in shoot tissue. The lipoxygenases initiated the plant oxylipin pathway and then produced jasmonic acid (JA), which participates in the defense responses against biotic and abiotic stresses [68–70]. Ethylene (ET) was also found to be involved in many abiotic stress responses . In this study, one cytokinin dehydrogenase was significantly up-regulated in the root tissue of Hua 11 by NaCl-treatment. Due to the fact that the concentrations of endogenous cytokine can be reduced by the cytokinin dehydrogenase, the antioxidative enzyme activity increases as cytokine decreases . Therefore, the barley salt stress tolerance may be improved by the enhancement of antioxidant enzymes. The result indicated that there is a potential link between salt stress and cytokinin signaling in barley.
According to the aforementioned, the salt stress signal transduction was very complex. These various signal molecules can act alone or together to improve the salt tolerance of barley.
4.2. Transcription Regulations under Salt Stress in Barley
Stress-related gene induction occurs principally at the transcriptional level, and regulation of the temporal and spatial expression patterns of specific stress-related genes is an important part of the plant stress response [73, 74]. In this research, a number of differentially expressed genes encoded transcriptional factors that were distinguished in the salt tolerant genotype of Hua 11. Among them, the ZIM motif family of genes, which is now known as TIFY family, was found to be responsive to one or more abiotic stresses . WRKY transcription factors have been confirmed to be involved in various abiotic stress responses [73, 76, 77]. The AP2/EREBP family of transcription factors was shown to mediate distinct responses to abiotic stresses by hormone-dependent gene expression . The bHLH (Basic Helix-Loop-Helix) proteins were found to play an important role in the ABA-mediated signal transduction pathway and in secondary metabolism , which also regulates the adaptive response of plants to abiotic stresses . In addition, NAC transcription factors have been shown to function in relation not only to plant development but also to abiotic and/or biotic stress responses . Whirly transcription factors are an important component of the SA (salicylic acid) signaling pathway-regulated defense of gene expression . The CBF (C-repeat binding factor) transcription factors, also known as DREB1s, were involved in responses to freezing, drought, and high-salt stress tolerance [83, 84]. The HD-Zip genes were potentially involved in both ABA-dependent and -independent drought-responsive signaling pathways .
Among the previously mentioned transcription factors, one C-repeat binding factor and one bHLH transcription factor showed a special pattern compared to other transcription factors. They were both up-regulated in the shoot tissue of Hua 11 and down-regulated in the shoot tissue of Hua 30 after 6 h of salt stress. They are, therefore, more likely to be related to salt tolerance than genes that are responsive only in Hua11 and not in Hua 30. The potential roles of these two transcription factors in salt tolerance should be further investigated. All of these transcription factors identified from the salt-tolerant genotype of Hua 11 showed complicated transcriptional regulatory networks of salt responses in barley, as they have been shown to perform crosstalk with phytohormones and secondary metabolism.
4.3. Compatible Solutes and Secondary Metabolites under Salt Stress in Barley
Under salt stress, crops can adjust their osmotic pressure by synthesizing compatible organic solutes to ease the damage brought upon by salt . Proline is one of the most widely studied compatible solutes, and its accumulation was observed in various organisms under conditions of salt stress [62, 86]. However, in this study, only one proline-related gene (Contig2524_s_at) was found in the shoot tissue of the salt-tolerant genotype Hua 11, which encoded a proline degradation enzyme. A reasonable explanation for this has not been found at present. Sucrose and fructose also have the ability to balance the osmotic potential of Na+ and Cl− .
The secondary metabolites have a protective role against biotic and abiotic environmental stresses . The flavonoid biosynthesis pathway was identified in rice salt-sensitive genotypes but not in salt-tolerant genotypes . However, in this study, the flavonoid biosynthesis pathway was induced not only in the salt-tolerant Hua 11 but also in the salt-sensitive Hua 30. The exact roles of flavonoids during barley salt stress are therefore not certain at present and need further study.
4.4. Specifically Affected Biochemical Pathways in Salt Tolerant Genotype
4.4.1. The Function of the Genetic Information Processing
Gene regulation is an essential process in the development and maintenance of a healthy body. The regulation of gene expression allows a cell to express specific proteins when needed to adapt to trigger developmental pathways, respond to environmental stimuli, and so on. In this study, four pathways involved in genetic information processing were significantly identified from the salt tolerant genotype, which means that the salt tolerant genotype could make an active response to salt stress. Meanwhile, the more differentially expressed genes were identified in salt tolerant genotype than sensitive genotype which also provides an evidence to support these results.
4.4.2. Jasmonic Acid (JA) Pathway Was Induced in Shoot of the Salt Tolerant Genotype
Jasmonic acid (JA) and its cyclic precursors and derivatives are collectively referred to as jasmonates (JAs). JAs are lipid-derived compounds with signal functions that mediate plant stress responses and development processes . Linoleic acid plays an important role as a precursor to the signal molecule of jasmonate . 13-LOX and 13-HPL pathway can produce Jasmonic acid . In our study, three genes were involved in Jasmonic acid biosynthesis pathway and have outstanding respone to salt stress in tolerant barley (Table 6). A similar observation has also been made in rice. Kang et al.  found that the concentration of endogenous JA in salt-tolerant rice was higher than salt-sensitive one at the various salt stress. Walia et al.  reported that salt stress can induce JA pathway-related genes in barley shoot tissue and the up-regulated lipoxygenase gene was also included. According to our result, identification of JA pathway is a prominent characteristic of the salt tolerant barley of Hua 11.
4.4.3. Lipid Metabolism and Salt Stress
Sphingolipids are not only a crucial part of the membrane lipids but also serve indispensable roles as signaling molecules that control several cellular processes, for example, stress responses . Very long chain fatty acids (VLCFA), which are essential precursors for sphingolipids, confer vital function in sphingolipid signaling . In our study, the sphingolipid metabolism and very long chain fatty acid biosynthesis were identified in shoot tissue. It is worth noting that among the four enzymes, 3-ketoacyl-CoA synthase (KCS) was the first rate-limiting step in the biosynthesis of very long chain fatty acids (VLCFA). Joubès et al.  found that 3-ketoacyl-CoA synthase 6 gene had an increased expression in response to the salt stress in Arabidopsis thaliana.
Phospholipids constitute the major part of cell membrane which is the first barrier that isolates cells from their environment and are a primary target for damage during abiotic stress . In response to reducing the abiotic stress damage, the organisms can increase the level of their unsaturated fatty acids to keep the appropriate fluidity of cell membrane by converting saturated fatty acids . It was reported that salinity stress could induce the high level expression of desaturase in yeast, which was consistent with as increased unsaturated fatty acids in the cell membranes . Overexpression of two omega-3 fatty acid desaturases (either FAD3 or FAD8) in tobacco could increase the various abiotic stresses including salt stress . Moreover the high-salt treatment also induces the accumulation of two omega-3 fatty acid desaturase genes transcripts (ZmFAD7 and ZmFAD8) in maize roots . In this study, one omega-3 fatty acid desaturase was up-regulated in root of tolerant genotype, which possibly increased the content of unsaturated fatty acids to maintain the fluidity of cell membrane under the salt stress.
4.4.4. Indole-3-Acetic Acid Homeostasis, and Salt Stress in Root Tissue
Indole-3-acetic acid (IAA) is an auxin that plays an important role in cell wall expansion, growth/development and responses to environmental stresses . In vascular plants, most IAA exists in a conjugated form that serves as a storage form of auxin . In present study, an IAA-amido synthetase that maintains auxin homeostasis by conjugating excess IAA to amino acids was identified from the root of salt tolerant genotype. The same result was also found in salt resistant maize, which can maintain IAA concentration in roots under the salt stress . The IAA homeostasis adaptation may provide a relatively favorable condition for the salt tolerance variety growth.
Using the transcriptional profiling of Barley1 chip, it was found that the salt tolerant barley: Hua 11 and salt sensitive barley: Hua 30 exhibited diverse transcriptional responses under the salt stress. In our research a lot of salt stress-related genes were induced by other abiotic stresses, supporting current view of cross talk between several kinds of abiotic stresses. Plants have evolved sophisticated mechanisms to respond positively to the various abiotic and biotic stress, thus the salt-responsive genes only up-regulated in the salt tolerant genotype might participate in the specific processes of salt tolerance in barley. These genes could serve as “candidate genes” not only for further comprehending of molecular mechanisms of salt stress tolerance but also for improving the salt tolerance of barely or other crops by gene manipulation. Pathway analysis unraveled that lipid metabolism plays an important role in shoot and root tissue of salt tolerant genotype to resistant to salt stress. Jasmonic acid pathway induced by salt stress had been found in previous study , which also supported by our result. Maybe it has a vital role for the salt tolerant genotype, which will be as an interesting point for the future research.
Besides, about 25% salt responsive genes identified from our experiment (24% in shoot and 28% in root) were homologous to function-unknown genes or without homologs, which probably play important roles in barley under the salt stress. These data will serve as a valuable resource for further salt stress study by methods of molecular genetics, such as RNA interference or the gene overexpression.
|MAPKs:||Mitogen activated protein kinases|
|CDPKs:||Calcium dependent protein kinases|
|CBL:||Calcineurin B-like protein|
|CIPKs:||CBL-interacting protein kinases|
|LysM RLK:||Lysine motif receptor-like kinase|
|PP2C:||Protein phosphatase 2C|
|CBF:||C-repeat binding factor.|
The authors thank Doctor Huang Fangliang of Zhejiang University for help in microarray hybridization and data analysis. The authors would also like to thank anonymous reviewers for comments on the paper. This work was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-05), the Project no. 7 from Science and High-Tech Based Major Program of Agriculture Committee of Shanghai Municipal Administration (Project no. 2012 7), the Project of Ministry of Agriculture of China, the Key Program of Basic Research of Shanghai Municipal S&T Commission of China (no. 12JC1407800), and the Science Foundation for Young Scientists of Shanghai Academy of Agricultural Sciences (Project no. 2012 15).
- S. Vij and A. K. Tyagi, “Emerging trends in the functional genomics of the abiotic stress response in crop plants: review article,” Plant Biotechnology Journal, vol. 5, no. 3, pp. 361–380, 2007.
- R. Munns, “Genes and salt tolerance: bringing them together,” New Phytologist, vol. 167, no. 3, pp. 645–663, 2005.
- K. Witzel, A. Weidner, G. K. Surabhi, A. Börner, and H. P. Mock, “Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity,” Journal of Experimental Botany, vol. 60, no. 12, pp. 3545–3557, 2009.
- R. Munns and M. Tester, “Mechanisms of salinity tolerance,” Annual Review of Plant Biology, vol. 59, pp. 651–681, 2008.
- J. C. Cushman and H. J. Bohnert, “Genomic approaches to plant stress tolerance,” Current Opinion in Plant Biology, vol. 3, no. 2, pp. 117–124, 2000.
- H. Knight and M. R. Knight, “Abiotic stress signalling pathways: specificity and cross-talk,” Trends in Plant Science, vol. 6, no. 6, pp. 262–267, 2001.
- L. Xiong, K. S. Schumaker, and J. K. Zhu, “Cell signaling during cold, drought, and salt stress,” Plant Cell, vol. 14, pp. S165–S183, 2002.
- S. Mahajan and N. Tuteja, “Cold, salinity and drought stresses: an overview,” Archives of Biochemistry and Biophysics, vol. 444, no. 2, pp. 139–158, 2005.
- J. K. Zhu, J. Liu, and L. Xiong, “Genetic analysis of salt tolerance in Arabidopsis: evidence for a critical role of potassium nutrition,” Plant Cell, vol. 10, no. 7, pp. 1181–1191, 1998.
- J. K. Zhu, “Regulation of ion homeostasis under salt stress,” Current Opinion in Plant Biology, vol. 6, no. 5, pp. 441–445, 2003.
- A. A. Ludwig, T. Romeis, and J. D. G. Jones, “CDPK-mediated signalling pathways: specificity and cross-talk,” Journal of Experimental Botany, vol. 55, no. 395, pp. 181–188, 2004.
- H. Nakagami, A. Pitzschke, and H. Hirt, “Emerging MAP kinase pathways in plant stress signalling,” Trends in Plant Science, vol. 10, no. 7, pp. 339–346, 2005.
- E. W. Chehab, J. V. Perea, B. Gopalan, S. Theg, and K. Dehesh, “Oxylipin pathway in rice and arabidopsis,” Journal of Integrative Plant Biology, vol. 49, no. 1, pp. 43–51, 2007.
- J. X. Liu, R. Srivastava, P. Che, and S. H. Howell, “Salt stress responses in Arabidopsis utilize a signal transduction pathway related to endoplasmic reticulum stress signaling,” Plant Journal, vol. 51, no. 5, pp. 897–909, 2007.
- S. Ma, Q. Gong, and H. J. Bohnert, “Dissecting salt stress pathways,” Journal of Experimental Botany, vol. 57, no. 5, pp. 1097–1107, 2006.
- M. Fujita, Y. Fujita, Y. Noutoshi et al., “Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks,” Current Opinion in Plant Biology, vol. 9, no. 4, pp. 436–442, 2006.
- L. Xiong and J. K. Zhu, “Molecular and genetic aspects of plant responses to osmotic stress,” Plant, Cell and Environment, vol. 25, no. 2, pp. 131–139, 2002.
- M. R. Junttila, S. P. Li, and J. Westermarck, “Phosphatase-mediated crosstalk between MAPK signaling pathways in the regulation of cell survival,” FASEB Journal, vol. 22, no. 4, pp. 954–965, 2008.
- A. A. Ludwig, H. Saitoh, G. Felix et al., “Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 30, pp. 10736–10741, 2005.
- N. Tuteja, “Abscisic acid and abiotic stress signaling,” Plant Signaling and Behavior, vol. 2, no. 3, pp. 135–138, 2007.
- N. Katerji, J. W. van Hoorn, A. Hamdy et al., “Classification and salt tolerance analysis of barley varieties,” Agricultural Water Management, vol. 85, no. 1-2, pp. 184–192, 2006.
- B. O. Bengtsson, “Barley genetics–not only here for the beer,” Trends in Genetics, vol. 8, no. 1, pp. 3–5, 1992.
- A. A. Jaradat, M. Shahid, and A. Al-Maskri, “Genetic diversity in the Batini barley landrace from Oman: II. Response to salinity stress,” Crop Science, vol. 44, no. 3, pp. 997–1007, 2004.
- H. Walia, C. Wilson, A. Wahid, P. Condamine, X. Cui, and T. J. Close, “Expression analysis of barley (Hordeum vulgare L.) during salinity stress,” Functional and Integrative Genomics, vol. 6, no. 2, pp. 143–156, 2006.
- A. M. Attumi, A study of salt tolerance in Arabidopsis thaliana and Hordeum vulgare [Ph.D. thesis], Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK, 2007.
- S. Kogenaru, Y. Qing, Y. P. Guo, and N. A. Wang, “RNA-seq and microarray complement each other in transcriptome profiling,” BMC Genomics, vol. 13, p. 629, 2012.
- H. Walia, C. Wilson, P. Condamine et al., “Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise,” BMC Genomics, vol. 8, article 87, 2007.
- H. Walia, C. Wilson, A. M. Ismail, T. J. Close, and X. Cui, “Comparing genomic expression patterns across plant species reveals highly diverged transcriptional dynamics in response to salt stress,” BMC Genomics, vol. 10, article 1471, p. 398, 2009.
- R. J. Lu, Z. W. Chen, T. He et al., “Relationship of NaCl tolerance between haploid cell level and plant level in two barely cultivars,” Journal of Nuclear Agricultural Sciences, vol. 25, no. 2, pp. 226–230, 2011.
- S. P. Lang and R. J. Lu, “Comparative study on salt tolerance of barley cultivars (lines) at seed germination stage,” Acta Agriculturae Shanghai, vol. 24, no. 4, pp. 83–87, 2008.
- Z. Z. Du, L. Zhou, Y. M. Zhang, Z. W. Chen, T. He, and J. H. Huang, “Effects of NaCl treatments on the growth quantity and physiological and biochemical indexes of barley after seed germination,” Acta Agriculturae Shanghai, vol. 26, no. 2, pp. 34–37, 2010.
- X. Zhang, J. Zhen, Z. Li et al., “Expression profile of early responsive genes under salt stress in upland cotton (Gossypium hirsutum L.),” Plant Molecular Biology Reporter, vol. 29, no. 3, pp. 626–637, 2011.
- A. Ueda, A. Kathiresan, J. Bennett, and T. Takabe, “Comparative transcriptome analyses of barley and rice under salt stress,” Theoretical and Applied Genetics, vol. 112, no. 7, pp. 1286–1294, 2006.
- Z. N. Ozturk, V. Talamé, M. Deyholos et al., “Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley,” Plant Molecular Biology, vol. 48, no. 5-6, pp. 551–573, 2002.
- J. J. Evans and N. A. Alldridge, “The distribution of peroxidases in extreme dwarf and normal tomato,” Phytochemistry, vol. 4, no. 3, pp. 499–503, 1965.
- M. J. Droillard, A. Paulin, and J. C. Marrot, “Free radical production, catalase and superoxide dismutase activities and membrane integrity during senescence of petals of cut carnations (Dianthus caryophyllus),” Physiologia Plantarum, vol. 71, pp. 197–202, 1987.
- L. Cheng, F. Wang, H. Shou et al., “Mutation in nicotianamine aminotransferase stimulated the Fe(II) acquisition system and led to iron accumulation in rice,” Plant Physiology, vol. 145, no. 4, pp. 1647–1657, 2007.
- Y. Benjamini and Y. Hochberg, “Controlling false discovery rate: a practical and powerful approach to multiple testing,” Journal of the Royal Statistical Society B, vol. 57, pp. 289–300, 1995.
- R. Mittler, S. Vanderauwera, M. Gollery, and F. Van Breusegem, “Reactive oxygen gene network of plants,” Trends in Plant Science, vol. 9, no. 10, pp. 490–498, 2004.
- S. S. Gill and N. Tuteja, “Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants,” Plant Physiology and Biochemistry, vol. 48, no. 12, pp. 909–930, 2010.
- O. Cotsaftis, D. Plett, A. A. T. Johnson et al., “Root-specific transcript profiling of contrasting rice genotypes in response to salinity stress,” Molecular Plant, vol. 4, no. 1, pp. 25–41, 2011.
- H. Walia, C. Wilson, P. Condamine et al., “Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage,” Plant Physiology, vol. 139, no. 2, pp. 822–835, 2005.
- E. Mangelsen, J. Kilian, K. Harter, C. Jansson, D. Wanke, and E. Sundberg, “Transcriptome analysis of high-temperature stress in developing barley caryopses: early stress responses and effects on storage compound biosynthesis,” Molecular Plant, vol. 4, no. 1, pp. 97–115, 2011.
- L. Xiong and M. Ishitani, “Stress signal transduction: components, pathways and network integration,” in Abiotic Stress Tolerance in Plants, A. K. Rai and T. Takabe, Eds., pp. 3–29, Springer, Dordrecht, The Netherlands, 2006.
- S. Q. Ouyang, Y. F. Liu, P. Liu et al., “Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice (Oryza sativa) plants,” Plant Journal, vol. 62, no. 2, pp. 316–329, 2010.
- S. A. Morillo and F. E. Tax, “Functional analysis of receptor-like kinases in monocots and dicots,” Current Opinion in Plant Biology, vol. 9, no. 5, pp. 460–469, 2006.
- T. Wu, Z. Tian, J. Liu, and C. Xie, “A novel leucine-rich repeat receptor-like kinase gene in potato, StLRPK1, is involved in response to diverse stresses,” Molecular Biology Reports, vol. 36, no. 8, pp. 2365–2374, 2009.
- S. W. Hong, J. H. Jon, J. M. Kwak, and H. G. Nam, “Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt, and cold treatments in Arabidopsis thaliana,” Plant Physiology, vol. 113, no. 4, pp. 1203–1212, 1997.
- R. Karlova, S. Boeren, E. Russinova, J. Aker, J. Vervoort, and S. De Vries, “The Arabidopsis somatic embryogenesis receptor-like kinase1 protein complex includes brassinosteroid-insensitive 1,” Plant Cell, vol. 18, no. 3, pp. 626–638, 2006.
- M. O. Santos and F. L. Aragão, “Role of SERK genes in plant environmental response,” Plant Signaling & Behavior, vol. 4, no. 12, pp. 1111–1113, 2009.
- M. Sivaguru, B. Ezaki, Z. H. He et al., “Aluminum induced gene expression and protein localization of a cell wall-associated receptor protein kinase in Arabidopsis thaliana,” Plant Physiology, vol. 132, no. 4, pp. 2256–2266, 2003.
- V. Kanneganti and A. K. Gupta, “Wall associated kinases from plants—an overview,” Physiology and Molecular Biology of Plants, vol. 14, no. 1-2, pp. 109–118, 2008.
- X. Hou, H. Tong, J. Selby, J. DeWitt, X. Peng, and Z. H. He, “Involvement of a cell wall-associated kinase, WAKL4, in Arabidopsis mineral responses,” Plant Physiology, vol. 139, no. 4, pp. 1704–1716, 2005.
- J. Wan, X. C. Zhang, D. Neece et al., “A LysM receptor-like kinase plays a critical role in chitin signaling and fungal resistance in Arabidopsis,” Plant Cell, vol. 20, no. 2, pp. 471–481, 2008.
- P. Gong, J. Zhang, H. Li et al., “Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction, and biochemical pathways in tomato,” Journal of Experimental Botany, vol. 61, no. 13, pp. 3563–3575, 2010.
- O. Šamajová, O. Plíhal, M. Al-Yousif, H. Hirt, and J. Šamaj, “Improvement of stress tolerance in plants by genetic manipulation of mitogen-activated protein kinases,” Biotechnology Advances, vol. 31, no. 1, pp. 118–128, 2013.
- J. J. Rudd and V. E. Franklin-Tong, “Calcium signaling in plants,” Cellular and Molecular Life Sciences, vol. 55, no. 2, pp. 214–232, 1999.
- J. J. Rudd and V. E. Franklin-Tong, “Unravelling response-specificity in Ca2+ signalling pathways in plant cells,” New Phytologist, vol. 151, no. 1, pp. 7–33, 2001.
- D. Sanders, C. Brownlee, and J. F. Harper, “Communicating with calcium,” Plant Cell, vol. 11, no. 4, pp. 691–706, 1999.
- R. F. Li, J. W. Zhang, J. H. Wei, H. Z. Wang, Y. Z. Wang, and R. C. Ma, “Functions and mechanisms of the CBL-CIPK signaling system in plant response to abiotic stress,” Progress in Natural Science, vol. 19, no. 6, pp. 667–676, 2009.
- Y. Jiang and M. K. Deyholos, “Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes,” BMC Plant Biology, vol. 6, article 25, 2006.
- M. K. Rai, R. K. Kalia, R. Singh, M. P. Gangola, and A. K. Dhawan, “Developing stress tolerant plants through in vitro selection-an overview of the recent progress,” Environmental and Experimental Botany, vol. 71, no. 1, pp. 89–98, 2011.
- M. Mazid, T. A. Khan, and F. Mohammad, “Role of secondary metabolites in defense mechanisms of plants,” Biology and Medicine, vol. 3, no. 2, pp. 232–249, 2011.
- P. L. Rodriguez, “Protein phosphatase 2C (PP2C) function in higher plants,” Plant Molecular Biology, vol. 38, no. 6, pp. 919–927, 1998.
- J. M. Pardo, M. P. Reddy, S. Yang et al., “Stress signaling through Ca2+/calmodulin-dependent protein phosphatase calcineurin mediates salt adaptation in plants,” Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 16, pp. 9681–9686, 1998.
- A. Schweighofer, H. Hirt, and I. Meskiene, “Plant PP2C phosphatases: emerging functions in stress signaling,” Trends in Plant Science, vol. 9, no. 5, pp. 236–243, 2004.
- Q. Xu, H. H. Fu, R. Gupta, and S. Luan, “Molecular characterization of a tyrosine-specific protein phosphatase encoded by a stress-responsive gene in Arabidopsis,” Plant Cell, vol. 10, no. 5, pp. 849–857, 1998.
- K. Cho, Y. C. Kim, J. C. Woo et al., “Transgenic expression of dual positional maize lipoxygenase-1 leads to the regulation of defense-related signaling molecules and activation of the anti-oxidative enzyme system in rice,” Plant Science, vol. 185-186, pp. 238–245, 2012.
- M. P. Rodríguez-Rosales, L. Kerkeb, P. Bueno, and J. P. Donaire, “Changes induced by NaCl in lipid content and composition, lipoxygenase, plasma membrane H+-ATPase and antioxidant enzyme activities of tomato (Lycopersicon esculentum. Mill) calli,” Plant Science, vol. 143, no. 2, pp. 143–150, 1999.
- A. Moons, E. Prinsen, G. Bauw, and M. Van Montagu, “Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots,” Plant Cell, vol. 9, no. 12, pp. 2243–2259, 1997.
- X. Y. Yang, J. X. Xie, X. P. Lu, Y. Z. Liu, and S. A. Peng, “Isolation of a citrus ethylene-responsive element binding factor gene and its expression in response to abiotic stress, girdling and shading,” Scientia Horticulturae, vol. 127, no. 3, pp. 275–281, 2011.
- Z. Mýtinová, V. Motyka, D. Haisel et al., “Antioxidant enzymatic protection during tobacco leaf ageing is affected by cytokinin depletion,” Plant Growth Regulation, vol. 65, pp. 23–34, 2011.
- L. G. Chen, Y. Song, S. J. Li, L. P. Zhang, C. S. Zou, and D. Q. Yu, “The role of WRKY transcription factors in plant abiotic stresses,” Biochimica et Biophysica Acta, vol. 1819, pp. 120–128, 2012.
- K. B. Singh, R. C. Foley, and L. Oñate-Sánchez, “Transcription factors in plant defense and stress responses,” Current Opinion in Plant Biology, vol. 5, pp. 430–436, 2002.
- H. Ye, H. Du, N. Tang, X. Li, and L. Xiong, “Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice,” Plant Molecular Biology, vol. 71, no. 3, pp. 291–305, 2009.
- S. P. Pandey and I. E. Somssich, “The role of WRKY transcription factors in plant immunity,” Plant Physiology, vol. 150, no. 4, pp. 1648–1655, 2009.
- P. J. Rushton, I. E. Somssich, P. Ringler, and Q. J. Shen, “WRKY transcription factors,” Trends in Plant Science, vol. 15, no. 5, pp. 247–258, 2010.
- D. Kizis, V. Lumbreras, and M. Pagès, “Role of AP2/EREBP transcription factors in gene regulation during abiotic stress,” FEBS Letters, vol. 498, no. 2-3, pp. 187–189, 2001.
- J. Kim and H. Y. Kim, “Molecular characterization of a bHLH transcription factor involved in Arabidopsis abscisic acid-mediated response,” Biochimica et Biophysica Acta, vol. 1759, no. 3-4, pp. 191–194, 2006.
- J. Zhou, F. Li, J. L. Wang, Y. Ma, K. Chong, and Y. Y. Xu, “Basic helix-loop-helix transcription factor from wild rice (OrbHLH2) improves tolerance to salt- and osmotic stress in Arabidopsis,” Journal of Plant Physiology, vol. 166, no. 12, pp. 1296–1306, 2009.
- K. Nakashima, H. Takasaki, J. Mizoi, K. Shinozaki, and K. Yamaguchi-Shinozaki, “NAC transcription factors in plant abiotic stress responses,” Biochimica et Biophysica Acta, vol. 1819, pp. 97–103, 2012.
- D. Desveaux, R. Subramaniam, C. Després et al., “A “Whirly” transcription factor is required for salicylic acid-dependent disease resistance in Arabidopsis,” Developmental Cell, vol. 6, no. 2, pp. 229–240, 2004.
- K. Yamaguchi-Shinozaki and K. Shinozaki, “Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters,” Trends in Plant Science, vol. 10, no. 2, pp. 88–94, 2005.
- Y. Ito, K. Katsura, K. Maruyama et al., “Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice,” Plant and Cell Physiology, vol. 47, no. 1, pp. 141–153, 2006.
- A. Agalou, S. Purwantomo, E. Övernäs et al., “A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members,” Plant Molecular Biology, vol. 66, no. 1-2, pp. 87–103, 2008.
- K. Sumithra, P. P. Jutur, B. D. Carmel, and A. R. Reddy, “Salinity-induced changes in two cultivars of Vigna radiata: responses of antioxidative and proline metabolism,” Plant Growth Regulation, vol. 50, no. 1, pp. 11–22, 2006.
- I. Türkan and T. Demiral, “Recent developments in understanding salinity tolerance,” Environmental and Experimental Botany, vol. 67, pp. 2–9, 2009.
- C. Wasternack and E. Kombrink, “Jasmonates: structural requirements for lipid-derived signals active in plant stress responses and development,” ACS Chemical Biology, vol. 5, no. 1, pp. 63–77, 2010.
- T. Nishiuchi and K. Iba, “Roles of plastid ω-3 fatty acid desaturases in defense response of higher plants,” Journal of Plant Research, vol. 111, no. 1104, pp. 481–486, 1998.
- M. Stumpe, J. G. Carsjens, I. Stenzel et al., “Lipid metabolism in arbuscular mycorrhizal roots of Medicago truncatula,” Phytochemistry, vol. 66, no. 7, pp. 781–791, 2005.
- D. J. Kang, Y. J. Seo, J. D. Lee et al., “Jasmonic acid differentially affects growth, ion uptake and abscisic acid concentration in salt-tolerant and salt-sensitive rice cultivars,” Journal of Agronomy and Crop Science, vol. 191, no. 4, pp. 273–282, 2005.
- T. M. Quist, I. Sokolchik, H. Shi et al., “HOS3, an ELO-like gene, Inhibits effects of ABA and implicates a S-1-P/ceramide control system for abiotic stress responses in Arabidopsis thaliana,” Molecular Plant, vol. 2, no. 1, pp. 138–151, 2009.
- J. Joubès, S. Raffaele, B. Bourdenx et al., “The VLCFA elongase gene family in Arabidopsis thaliana: phylogenetic analysis, 3D modelling and expression profiling,” Plant Molecular Biology, vol. 67, no. 5, pp. 547–566, 2008.
- S. Rodríguez-Vargas, A. Sánchez-García, J. M. Martínez-Rivas, J. A. Prieto, and F. Randez-Gil, “Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress,” Applied and Environmental Microbiology, vol. 73, no. 1, pp. 110–116, 2007.
- T. Sakamoto and N. Murata, “Regulation of the desaturation of fatty acids and its role in tolerance to cold and salt stress,” Current Opinion in Microbiology, vol. 5, no. 2, pp. 206–210, 2002.
- C. Gostinčar, M. Turk, T. Trbuha, T. Vaupotič, A. Plemenitaš, and N. Gunde-Cimerman, “Expression of fatty-acid-modifying enzymes in the halotolerant black yeast Aureobasidium pullulans (de Bary) G. Arnaud under salt stress,” Studies in Mycology, vol. 61, pp. 51–59, 2008.
- M. Zhang, R. Barg, M. Yin et al., “Modulated fatty acid desaturation via overexpression of two distinct ω-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants,” Plant Journal, vol. 44, no. 3, pp. 361–371, 2005.
- T. Berberich, M. Harada, K. Sugawara, H. Kodama, K. Iba, and T. Kusano, “Two maize genes encoding ω-3 fatty acid desaturase and their differential expression to temperature,” Plant Molecular Biology, vol. 36, no. 2, pp. 297–306, 1998.
- C. Zorb, C. M. Geilfus, K. H. Muhling, and J. Ludwig-Muller, “The influence of salt stress on ABA and auxin concentrations in two maize cultivars differing in salt resistance,” Journal of Plant Physiology, vol. 170, no. 2, pp. 220–224, 2013.
- J. D. Cohen and R. S. Bandurski, “The chemistry and physiology of the bound auxins,” Annual Review of Plant Physiology, vol. 33, pp. 403–430, 1982.