Cyanamide Potentiates the Ethanol-Induced Impairment of Receptor-Mediated Endocytosis in a Recombinant Hepatic Cell Line Expressing Alcohol Dehydrogenase Activity
Figure 1
(a) Effects of ethanol (25 mM) and cyanamide (0.1 mM) on binding of 125I-ASOR to HAD cells treated for 7 days. HAD cells were grown to confluence and treated as described in Section 2. Ligand binding was performed at 4°C for 3 hours and non-specific binding was determined in the presence of 100-fold excess unlabeled ligand. Data are presented as means ± SEM for 6–10 sets of cells. Legends: control: HAD cells cultured in the absence of added ethanol; ethanol: HAD cells cultured in the presence of 25 mM ethanol. Values significantly different from controls are indicated, . (b) Effects of ethanol administration (25 mM) and cyanamide (0.1 mM) on binding of anti-ASGP-R antibody to HAD cells. Cell cultures were obtained from the same conditions as in Figure 1(a). Binding of antibody was determined by the ability of cells to bind a polyclonal antihuman ASGP-receptor antibody, followed by radiolabeled detection of 125I-protein A. Data are presented as means ± SEM for 6–10 experiments. Legends: control: HAD cells cultured in the absence of added ethanol; ethanol: HAD cells cultured in the presence of 25 mM ethanol.
