Research Article
The O28 Antigen Gene Clusters of Salmonella enterica subsp. enterica Serovar Dakar and Serovar Pomona Are Different
Table 2
Putative genes and proteins comprising the O antigen cluster of S. Pomona isolate 07-0213.
| ORF | Position in Sequence (nt) | Length (bp) | %G + C | % DNA identity | Species with the closest DNA identity | Accession No. | Protein length (aa) | Similar protein | Protein Function | Species with closest protein homolog | % amino acid identity | Accession No. of closest homolog |
| 1 | 101–1174 | 1074 | 42.92 | 97 | S. Paratyphi A AKU_12601 | FM200053.1 | 357 | RmlB | dTDP-glucose 4,6 dehydratase | S. Typhi CT18 | 96 | NP_456646.1 | 2 | 1176–2039 | 864 | 37.38 | 76 | E. coli O103:H2 strain 12009 | AP010958.1 | 287 | RmlA | D-glucose-1-phosphate thymidylyltransferase | E. coli 101-1 | 86 | ZP_03068444.1 | 3 | 2043–2438 | 396 | 33.08 | 71 | E. coli serogroup O91 | AY035396.1 | 131 | FdtA | dTDP-6-deoxy-3,4-keto-hexulose isomerase | Pectobacterium carotovorum subsp. brasiliensis PBR1692 | 70 | ZP_03826801.1 | 4 | 2435–2902 | 466 | 35.68 | 69 | E. coli serogroup O2 | EU549863.1 | 154 | FdtC | acetyltransferase | Providencia rettgeri DSM 1131 | 68 | ZP_06127571.1 | 5 | 2899–4002 | 1105 | 36.05 | 68 | E. coli strain H515b | EF027106.1 | 368 | FdtB | aminotransferase | E. coli 101-1 | 83 | ZP_03068415.1 | 6 | 4005–5261 | 1257 | 30.15 | — | none | — | 418 | Wzx | O-antigen flippase | E. coli 101-1 | 80 | ZP_03068086.1 | 7 | 5262–6578 | 1317 | 29.31 | — | none | — | 438 | Wzy | oligosaccharide repeat unit polymerase | E. coli 101-1 | 75 | ZP_03068123.1 | 8 | 6565–7440 | 876 | 29.79 | 71% over 122 nt | Pasteurella multocida subsp. multocida strain Pm70 | AE004439.1 | 292 | WbuM | glycosyltransferase group 2 family | E. coli 101-1 | 77 | ZP_03068240.1 | 9 | 7440–8123 | 684 | 29.09 | — | none | — | 227 | WbuN | phosphoserine phosphatase | E. coli O114 | 43 | AAT77178.1 | 10 | 8110–8901 | 792 | 26.44 | — | none | — | 263 | WbuO | O-antigen acyltransferase | E. coli 101-1 | 67 | ZP_03068384.1 | 11 | 8894–9718 | 825 | 34.18 | 74 (128 nt) | E. coli O117 | EU694096.1 | 274 | AmsE | amylovoran biosynthesis glycosyltransferase | E. coli 101-1 | 56 | ZP_03068341.1 |
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