- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Article Processing Charges ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Recently Accepted Articles ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
International Journal of Microbiology
Volume 2011 (2011), Article ID 709509, 6 pages
The Role of IL-17 in the Association between Pneumococcal Pneumonia and Allergic Sensitization
1Division of Community Pediatric and Adolescent Medicine, Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN, USA
2Department of Pediatrics, Shenzhen Maternal and Child Health Care Hospital Affiliated to Nanfang Medical University, Shenzhen 518028, China
3Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea
4Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN, USA
5Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA
6Division of Allergic Diseases, Department of Medicine, Mayo Clinic, Rochester, MN, USA
7Department of Pediatric and Adolescent Medicine, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
Received 26 July 2011; Accepted 7 September 2011
Academic Editor: William M. Shafer
Copyright © 2011 Hongxia Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Interleukin- (IL-) 17 is important in the development of asthma and host defense against pneumococci. We determined the role of IL-17 in the risk of pneumococcal pneumonia. We challenged mice intranasally with a bioluminescent Streptococcus pneumoniae strain after sensitization and challenge with ovalbumin (OVA). We measured the levels of cytokines, including IL-17 (pg/mL), in the lung homogenate in experimental mice with and without OVA sensitization/challenge, as well as those with and without pneumococcal pneumonia. IL-17 levels were significantly lower in OVA-sensitized/challenged mice (9.69 1.49), compared to the control mice (20.92 1.82, < 0.001). In our overall analysis, including IL-4 and IL-17 levels and OVA sensitization/challenge, IL-4 levels (OR: 81.9, 95%CI: 4.3–1523 per increment of 1.0 pg/mL, = 0.003) were more significant than IL-17 levels (OR: 1.1, 95%CI: 1.03–1.17 per increment of 1.0 pg/mL, = 0.003) in determining the risk of pneumococcal pneumonia. IL-17 levels result in a much smaller impact on the risk for pneumococcal pneumonia, compared to IL-4 levels.
Streptococcus pneumoniae is a leading cause of bacterial pneumonia, meningitis, and sepsis and has caused substantial morbidity and mortality in the United States and worldwide, especially in young children. Recent estimates of childhood deaths caused by Streptococcus pneumoniae range from 70,000 to 1 million every year worldwide, and S. pneumoniae caused around 11% of all deaths in children aged 1–59 months . In 2000, about 14.5 million episodes of serious pneumococcal disease were estimated to occur, and pneumococcal disease caused about 826,000 deaths in children aged 1–59 months . In the US, 40,000 cases of fatal pneumococcal infections per year have been reported . Recently some studies reported that individuals with asthma have a significantly increased risk of invasive pneumococcal disease, compared to those without asthma [3, 4]. Thus, the Advisory Committee on Immunization Practices has a recommendation for the use of 23-valent pneumococcal polysaccharide vaccine (PPV23) for the prevention of invasive pneumococcal disease in asthmatics . At present, little is known about the mechanisms underlying the increased risk of invasive pneumococcal disease among individuals with asthma. We previously reported that a T-helper 2 (Th2) predominant immune response (e.g., IL-4) to OVA sensitization was a significant risk factor for pneumococcal infection, whereas allergic sensitization itself was protective against pneumococcal pneumonia . Others also reported that IL-4 was associated with diminished host defense against Pseudomonas lung infection in mice . Therefore, while Th2 cytokines such as IL-4 are associated with a risk of bacterial pneumonia in mice, how allergic sensitization provides a protective effect against pneumococcal pneumonia in mice is not well understood. One potential hypothesis is that allergic sensitization-induced IL-17 may provide a protective effect against pneumococcal disease because IL-17 is a cytokine that enhances host defense against pneumococcal infection. T-helper 17 (Th17) cells are a newly discovered CD4+ helper T-cell subset that produce IL-17 and are involved in host defense against bacterial infection, mainly through the recruitment and activation of neutrophils . IL-17 is an important inflammatory mediator in the development of asthma [9, 10] and protection from pneumococcal colonization [11, 12]. Indeed, recent studies report that allergic sensitization with OVA in mice induced secretion of IL-17 from macrophages and elevation of IL-17 levels in BAL fluid [10, 13], but production of IL-17 by Th17 cells (particularly IL-23-dependent IL-17 secretion) was negatively regulated by IL-4 . Therefore, we hypothesize that elevated IL-17 levels in BAL induced by allergic sensitization may provide a protective effect on the development of pneumococcal pneumonia. To test this hypothesis, we investigated the role of IL-17 in both airway inflammation and pneumococcal pneumonia using a murine model.
2. Materials and Methods
Experimental details have been previously reported  the study was approved by the Mayo Clinic IACUC. Briefly, pathogen-free 6–8-week-old female BALB/c mice were sensitized by intraperitoneal (i.p.) injection of 20 μg OVA adsorbed to 1 mg aluminum hydroxide gel (in a volume of 100 μL) on days 0 and 7. Then, experimental mice were intranasally challenged with 100 μg OVA in 50 μL phosphate-buffered saline (PBS) under light tribromoethanol anesthesia on days 15, 16, and 17. Control mice received i.p. injection of PBS with aluminum hydroxide gel but no intranasal challenge. Three days after OVA challenge, the OVA-sensitized/challenged mice and control mice were challenged with S. pneumoniae A 66.1 serotype 3 (1.5 × 104 to 3 × 104 cfu), made bioluminescent by integration of a modified lux operon into its chromosome (Xen 10, Caliper Life Sciences, Hopkinton, Mass, USA) . Pneumococcal pneumonia was defined as the presence of bioluminescence and positive pneumococcal culture from normally sterile body tissues (spleen or brain). Cytokine and chemokine levels in the lung tissues were determined by Bio-Plex or ELISA assays.
2.2. Outcome Measures and Statistical Analysis
To examine the impact of OVA sensitization/challenge on IL-17 levels in the lung, we compared IL-17 levels between OVA-sensitized/challenged and control mice after stratification into mice that developed pneumococcal pneumonia and those that did not. To assess the role of IL17 in the risk of pneumococcal disease, we compared IL-17 levels between mice with and without pneumococcal pneumonia and determined the correlation between IL-17 levels and colony counts of pneumococci from lung and spleen (log10 CFU) using Pearson’s correlation coefficient. To determine the relationship between IL-4 and IL-17, we calculated Pearson’s correlation coefficient between IL-17 and Th2 cytokines.
To examine whether the impact of IL-17 on pneumococcal infection is independent of sensitization and challenge with OVA and IL-4, data were fitted to a logistic regression. We calculated odds ratios (ORs) and corresponding 95% confidence intervals for OVA sensitization/challenge, IL-17 levels, IL-4 levels, and the interaction term between OVA sensitization/challenge and IL-4 to identify factors associated with the risk of pneumococcal pneumonia. All statistical significance was tested at a two-tailed alpha error of 0.05.
3.1. Relationship between IL-17 Levels in Lung, OVA Sensitization/Challenge, and Pneumococcal Pneumonia
IL-17 levels were significantly lower in OVA-sensitized/challenged mice, compared to the control mice (9.69 ± 1.49 versus 20.92 ± 1.82 pg/mL, ) and were elevated in mice with compared to those without pneumococcal pneumonia (20.53 ± 1.96 versus 10.28 ± 1.44 pg/mL, ).
The results stratified by pneumococcal pneumonia status are summarized in Table 1. IL-17 level was lower in OVA-sensitized/challenged mice, compared to control mice in the mice without pneumococcal pneumonia (). However, there was no difference in IL-17 levels between OVA-sensitized/challenged mice and control mice in mice with pneumococcal pneumonia (). In OVA-sensitized/challenged mice, the IL-17 level was higher in mice that developed pneumococcal pneumonia than those that did not (). However, in the control mice, the IL-17 level was not associated with pneumococcal pneumonia ().
3.2. Correlation between IL-17 Levels and Other Cytokines and Chemokines in Lung
IL-17 levels were positively correlated with IL-1α (), IL-13 (0.32), and RANTES () levels in the lungs of sensitized mice (), whereas IL-17 levels were inversely correlated with IL-4 levels in the lungs of mice without pneumococcal pneumonia, a finding which approached statistical significance (, ). There was no correlation between IL-17 and other cytokines (IL-1, IL-2, IL-5, IL-6, IL-10, IFN-γ, and TNF-α) or chemokines (GM-CSF, EOTAXIN, and MIP-1) in lung homogenates.
3.3. Factors Associated with the Risk of Pneumococcal Pneumonia
The results on the influence of IL-17, IL-4, and OVA sensitization/challenge on the risk of pneumococcal pneumonia are summarized in Table 2. The mean level (± standard error) of IL-4 in lung homogenates among mice with pneumococcal pneumonia was 1.09 ± 0.14 pg/mL whereas that among mice without pneumococcal pneumonia was 0.67 ± 0.06 pg/mL (). Therefore, adjusting for the impact of IL-4 and OVA sensitization/challenge on the risk on pneumococcal pneumonia, IL-17 minimally influenced the risk of pneumococcal pneumonia. OVA sensitization/challenge was not significantly associated with the risk of pneumococcal pneumonia, but, as we previously reported, IL-4 was still significantly associated with the risk of pneumococcal pneumonia adjusting for IL-17 and OVA sensitization/challenge.
In our study, sensitization/challenge with OVA reduced the risk of pneumococcal pneumonia (28% versus 57%) and the number of pneumococci in the lung homogenates of mice with pneumococcal pneumonia, compared to control mice. This reduced risk of pneumococcal pneumonia in OVA-sensitized/challenged mice was minimally accounted for by IL-17 levels in lung, but IL-4 levels in lung were more significantly associated with the risk of pneumococcal pneumonia than IL-17.
Our results suggest that IL-17 levels were lower in OVA-sensitized/challenged mice than the control mice, particularly in mice without pneumococcal pneumonia. However, it is uncertain whether OVA sensitization/challenge failed to induce IL-17 since we did not have a baseline measure of IL-17. However, Th2 cytokines showed an inconsistent relationship with IL-17 levels. There was no correlation between IL-5 and IL-17 levels. IL-4 was inversely correlated with IL-17 levels whereas IL-13 was positively correlated with IL-17 levels in the lung. Therefore, in our mouse model, allergic sensitization might not significantly increase IL-17 levels in the lung homogenate but the inconsistent effects of Th2 cytokines on IL-17 levels. Previous studies reported that OVA sensitization/challenge increases IL-17 levels in lung [9, 10]. However, Schnyder-Candrian et al. reported that IL-17 has a dual role: it is essential during antigen sensitization to establish allergic asthma, but, in sensitized mice, IL-17 attenuates the allergic response by inhibiting chemokines . In our study, IL-17 levels in the lung were positively correlated with IL-1 levels but inversely correlated with IL-4 levels, findings which are consistent with the literature [14, 16–18].
For the relationship between IL-17 levels in the lung and the risk of pneumococcal pneumonia, in our study, IL-17 levels in the lung were associated with an increased risk of pneumococcal pneumonia, particularly in OVA-sensitized/challenged mice. IL-17 levels in the lung are positively correlated with pneumococcal colony counts from lung and spleen as summarized in Figures 1 and 2, and there was a positive association between IL-17 levels and pneumococcal pneumonia as summarized in Tables 1 and 2. In interpreting these results, IL-17 levels in the lung might reflect host response to pneumococcal pneumonia (i.e., IL-17 levels induced by pneumococcal pneumonia) instead of causing an increased risk of pneumococcal pneumonia. However, we observed increased IL-17 levels in mice with pneumococcal pneumonia only among the group of OVA-sensitized/challenged mice, but IL-17 levels did not differ between mice with and without pneumococcal pneumonia in the control mice as shown in Table 1. Also, IL-17 levels were not correlated with pneumococcal colony counts in lung and spleen in the control mice. Therefore, IL-17 levels in the lung might not solely reflect the host response to pneumococcal pneumonia. The role of IL-17 in host defense from microbial infections including pneumococcal infection has been well supported by the literature [11, 12, 19, 20]. IL-17 participates in host defense through regulation of innate immunity (e.g., recruitment of neutrophils and induction of antimicrobial peptides) and cell-mediated immunity (e.g., T-cell activation) [8, 20]. However, our study results were in contrast with these studies and suggest a potential adverse effect of IL-17 on host defense. Our study is limited in addressing this unexpected finding, and we can only speculate as to the potential explanation. As an organism-specific mechanism, a recent study reported that IL-17 up-regulates expression of polymeric immunoglobulin receptor (PIgR) by epithelial cells, a receptor for pneumococcal cholin-binding protein A (CbpA) . CbpA has been known as an important pneumococcal adhesin to certain receptors (e.g., PIgR as well as platelet-activating factor receptor) to invade through reverse epithelial transcytosis [22, 23]. Thus, upregulation of PIgR may increase binding of pneumococci to the epithelial cell surface and allow pneumococci to invade epithelial cells and inner tissues, resulting in serious infections . Also, Th2 cytokines such as IL-4 upregulate PIgR and may further enhance invasion of pneumococci in the presence of IL-17 which may account for the positive correlation between IL-17 and pneumococcal colony count in the lung and spleen only among OVA-sensitized/challenged but not control mice, as observed in our study . As a nonspecific mechanism for the negative influence of IL-17 on the risk of pneumococcal pneumonia, IL-17 has been reported to promote inflammation by inducing various proinflammatory cytokines and chemokines, recruiting neutrophils, enhancing antibody production, and activating T cells . The etiologic involvement of IL-17 in certain autoimmune conditions such as rheumatoid arthritis  and multiple sclerosis  has been suggested. Therefore, IL-17 might result in significant airway inflammation damaging lung architecture and making the host susceptible to microbial infections. Indeed, previous studies showed that IL-17 was associated with increased risks of Aspergillus fumigatus infection and diminished antifungal immunity . However, in our study, the overall impact of IL-17 on the risk of pneumococcal pneumonia was minimal (OR: 1.10), taking into account the role of IL-4 and OVA sensitization/challenge as summarized in Table 2.
As we previously reported, IL-4 levels in the lung were still a significant factor for the risk of pneumococcal pneumonia in our mouse model, adjusting for the role of IL-17 and OVA sensitization/challenge. The adverse effect of IL-4 on the risk of microbial infections has been well supported by the literature [28, 29]. Thus, as discussed above, given the dual effects (protective and provocative) of IL-17 on the risk of pneumococcal pneumonia, IL-4 (and attenuated production of proinflammatory cytokines by reciprocal inhibition by Th2 cytokines) may play a more important role in determining the risk of pneumococcal pneumonia than IL-17.
The main strengths of our study were a large sample size of mice ensuring sufficient statistical power and use of a luminescence technique allowing us to identify pneumococcal pneumonia without sacrificing mice. Our study also had limitations. There was no measurement for IL-17 levels in the lung at baseline (time zero), and IL-17 levels were measured at different time points depending on disease progress of mice, which may influence the interpretation of the results. Another limitation of the animal model of this study was acute airway inflammation status, and the result might not be applicable for asthma in human, a chronic airway inflammation.
In conclusion, our study results highlight that allergic sensitization may not induce IL-17, and IL-17 levels in the lung may result in a negative influence on host defense against pneumococcal pneumonia, albeit minimal. However, IL-4 levels in the lung are still more important for determining the risk of pneumococcal pneumonia than IL-17 and allergic sensitization. Given the limitations of our study, further studies are needed to clarify the role of IL-17 in the risk of pneumococcal disease.
|95%CI:||95% confidence interval|
|CFU:||Colony forming unit|
The authors thank Diane Squillace for laboratory support and Cheryl Adolphson and Kathy Disdad for administrative assistance. The study was supported by the Bridge Award from the Mayo Foundation. The investigators have nothing to disclose that poses a conflict of interest.
- K. L. O'Brien, L. J. Wolfson, J. P. Watt et al., “Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates,” The Lancet, vol. 374, no. 9693, pp. 893–902, 2009.
- D. Bogaert, M. N. Engelen, A. J. M. Timmers-Reker et al., “Pneumococcal carriage in children in The Netherlands: a molecular epidemiological study,” Journal of Clinical Microbiology, vol. 39, no. 9, pp. 3316–3320, 2001.
- T. R. Talbot, T. V. Hartert, E. Mitchel et al., “Asthma as a risk factor for invasive pneumococcal disease,” New England Journal of Medicine, vol. 352, no. 20, pp. 2082–2090, 2005.
- Y. J. Juhn, H. Kita, B. P. Yawn et al., “Increased risk of serious pneumococcal disease in patients with asthma,” Journal of Allergy and Clinical Immunology, vol. 122, no. 4, pp. 719–723, 2008.
- The Advisory Committee on Immunization Practices, “ACIP provisional recommendations foruse of pneumococcal vaccines,” 2008, http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5934a3.htm.
- C. I. Kang, M. S. Rouse, R. Patel, H. Kita, and Y. J. Juhn, “Allergic airway inflammation and susceptibility to pneumococcal pneumonia in a murine model with real-time in vivo evaluation,” Clinical and Experimental Immunology, vol. 156, no. 3, pp. 552–561, 2009.
- C. Moser, H. K. Johansen, Z. Song, H. P. Hougen, J. Rygaard, and N. Høiby, “Chronic Pseudomonas aeruginosa lung infection is more severe in Th2 responding BALB/c mice compared to Th1 responding C3H/HeN mice,” APMIS, vol. 105, no. 11, pp. 838–842, 1997.
- M. Kawaguchi, M. Adachi, N. Oda, F. Kokubu, and S. K. Huang, “IL-17 cytokine family,” Journal of Allergy and Clinical Immunology, vol. 114, no. 6, pp. 1265–1273, 2004.
- R. H. Wilson, G. S. Whitehead, H. Nakano, M. E. Free, J. K. Kolls, and D. N. Cook, “Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness,” American Journal of Respiratory and Critical Care Medicine, vol. 180, no. 8, Article ID 200904, pp. 720–730, 2009.
- S. Schnyder-Candrian, D. Togbe, I. Couillin et al., “Interleukin-17 is a negative regulator of established allergic asthma,” Journal of Experimental Medicine, vol. 203, no. 12, pp. 2715–2725, 2006.
- Z. Zhang, T. B. Clarke, and J. N. Weiser, “Cellular effectors mediating Th17-dependent clearance of pneumococcal colonization in mice,” Journal of Clinical Investigation, vol. 119, no. 7, pp. 1899–1909, 2009.
- Y. J. Lu, J. Gross, D. Bogaert et al., “Interleukin-17A mediates acquired immunity to pneumococcal colonization,” PLoS Pathogens, vol. 4, no. 9, Article ID e1000159, 2008.
- H. Park, Z. Li, X. O. Yang et al., “A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17,” Nature Immunology, vol. 6, no. 11, pp. 1133–1141, 2005.
- H. Hoshino, M. Laan, M. Sjöstrand, J. Lötvall, B. E. Skoogh, and A. Lindén, “Increased elastase and myeloperoxidase activity associated with neutrophil recruitment by IL-17 in airways in vivo,” Journal of Allergy and Clinical Immunology, vol. 105, no. 1 I, pp. 143–149, 2000.
- K. P. Francis, J. Yu, C. Bellinger-Kawahara et al., “Visualizing pneumococcal infections in the lungs of live mice using bioluminescent Streptococcus pneumoniae transformed with a novel gram-positive lux transposon,” Infection and Immunity, vol. 69, no. 5, pp. 3350–3358, 2001.
- S. Z. Ben-Sasson, J. Hu-Li, J. Quiel et al., “IL-1 acts directly on CD4 T cells to enhance their antigen-driven expansion and differentiation,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 17, pp. 7119–7124, 2009.
- Y. Chung, S. H. Chang, G. J. Martinez et al., “Critical regulation of early Th17 cell differentiation by Interleukin-1 signaling,” Immunity, vol. 30, no. 4, pp. 576–587, 2009.
- L. Lyakh, G. Trinchieri, L. Provezza, G. Carra, and F. Gerosa, “Regulation of interleukin-12/interleukin-23 production and the T-helper 17 response in humans,” Immunological Reviews, vol. 226, no. 1, pp. 112–131, 2008.
- S. C. Liang, X. Y. Tan, D. P. Luxenberg et al., “Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides,” Journal of Experimental Medicine, vol. 203, no. 10, pp. 2271–2279, 2006.
- G. Matsuzaki and M. Umemura, “Interleukin-17 as an effector molecule of innate and acquired immunity against infections,” Microbiology and Immunology, vol. 51, no. 12, pp. 1139–1147, 2007.
- Z. Jaffar, M. E. Ferrini, L. A. Herritt, and K. Roberts, “Cutting edge: lung mucosal Th17-mediated responses induce polymeric Ig receptor expression by the airway epithelium and elevate secretory IgA levels,” Journal of Immunology, vol. 182, no. 8, pp. 4507–4511, 2009.
- C. S. Kaetzel, “The polymeric immunoglobulin receptor: bridging innate and adaptive immune responses at mucosal surfaces,” Immunological Reviews, vol. 206, pp. 83–99, 2005.
- R. Luo, B. Mann, W. S. Lewis et al., “Solution structure of choline binding protein A, the major adhesin of Streptococcus pneumoniae,” EMBO Journal, vol. 24, no. 1, pp. 34–43, 2005.
- Y. Iwakura, S. Nakae, S. Saijo, and H. Ishigame, “The roles of IL-17A in inflammatory immune responses and host defense against pathogens,” Immunological Reviews, vol. 226, no. 1, pp. 57–79, 2008.
- M. I. Koenders, L. A. B. Joosten, and W. B. Van Den Berg, “Potential new targets in arthritis therapy: interleukin (IL)-17 and its relation to tumour necrosis factor and IL-1 in experimental arthritis,” Annals of the Rheumatic Diseases, vol. 65, supplement 3, pp. iii29–iii33, 2006.
- L. A. Tesmer, S. K. Lundy, S. Sarkar, and D. A. Fox, “Th17 cells in human disease,” Immunological Reviews, vol. 223, no. 1, pp. 87–113, 2008.
- T. Zelante, A. De Luca, P. Bonifazi et al., “IL-23 and the Th17 pathway promote inflammation and impair antifungal immune resistance,” European Journal of Immunology, vol. 37, no. 10, pp. 2695–2706, 2007.
- C. Beisswenger, K. Kandler, C. Hess et al., “Allergic airway inflammation inhibits pulmonary antibacterial host defense,” Journal of Immunology, vol. 177, no. 3, pp. 1833–1837, 2006.
- A. Q. Khan, Y. Shen, Z. Q. Wu, T. A. Wynn, and C. M. Snapper, “Endogenous pro- and anti-inflammatory cytokines differentially regulate an in vivo humoral response to Streptococcus pneumoniae,” Infection and Immunity, vol. 70, no. 2, pp. 749–761, 2002.