Schwann Cell Metabolic Activity in Various Short-Term Holding Conditions: Implications for Improved Nerve Graft Viability
Figure 4
Schwann cell metabolism indicated as absorbance measured with MTS at 4°C (a), room temperature (b), and at 37°C (c) incubated in the described media. At 4°C all conditions resulted after 3 hours in massive reduction of cell viability. At room temperature incubation in HRBM and DMEM showed slightly better results than NaCl and Leibovitz’s. Optimal cell viability with increase of Schwann cell metabolism can be seen after incubation in HBRM at 37°C as observed by independent determination of cell viability by CTB in Figure 2. .
