Application of Phosphoproteomics to Find Targets of Casein Kinase 1 in the Flagellum of Chlamydomonas

Table 2

Additional phosphopeptides/phosphorylation sites in CKI-7-treated cells of either novel phosphoproteins or phosphoproteins that were already identified in 137c [11].

Phosphoproteins only present in CKI-7-treated 137c cells
Glycogen synthase kinase 3; GSK3
Kinesin-like protein; Kinesin motor domain, KIF9-like subgroup
Phosphoglucomutase
Radial spoke protein 11; RSP11; RIIa domain
S-Adenosylmethionine synthetase
FAP139^a; TIGR02680 domain
FAP21^a
FAP56
FAP75
FAP98
FAP129
FAP165^a
FAP236
FAP241
FAP243 (Vs3 FAP183)^a
Phosphoproteins found in CKI-7-treated cells with additional phosphopeptide(s) in comparison to 137c
Outer dynein arm docking complex protein 2; ODA-DC2
FAP33^a,b; ankyrin repeats
FAP154^a
FAP217
Phosphoproteins found in CKI-7-treated cells with the same peptide [11] but with additional phosphorylation site(s)
FAP39^a; plasma membrane calcium transporting ATPase
MAK7^a; mitogen activated protein kinase 7

The function of depicted proteins is given as determined by NCBI BLASTp, along with their conserved domains.
^aVs2 model differs significantly from Vs3 model.
^bPredicted functional domains are present only in the Vs3 model.