Modulation of Neutrophil Apoptosis by Antimicrobial Peptides

Figure 3

Effects of FPRL1- and P2X₇-antagonists on the LL-37-induced suppression of neutrophil apoptosis. Neutrophils (10⁶ cells/mL) were incubated for 18 h at 37°C in RPMI1640-10% FBS in the absence (Control) or presence of LL-37 (1 μg/mL), WRW⁴ (10 μM), oxidized ATP (Ox-ATP, 100 μM), KN-93 (5 μM) or their combination (+LL-37; 10 μM WRW⁴ and 1 μg/mL LL-37, 100 μM Ox-ATP and 1 μg/mL LL-37 or 5 μM KN-93 and 1 μg/mL LL-37). Neutrophils were also incubated for 18 h at 4°C in the absence of LL-37, FPRL1-, or P2X₇-antagonists (Resting). After incubation, apoptosis of neutrophils was quantitated and expressed as a percentage of apoptotic cells. Data are the mean ± SD of 4 to 12 separate experiments. Values are compared between the incubation at 37°C in the absence (Control) and presence of LL-37, WRW⁴, oxidized ATP, KN-93 or their combination. ***𝑃<0.001.