About this Journal Submit a Manuscript Table of Contents
ISRN Analytical Chemistry
Volume 2012 (2012), Article ID 617175, 12 pages
Research Article

Utility of p-Chloranilic Acid and 2,3-Dichloro-5,6-dicyano-p-benzoquinone for the Spectrophotometric Determination of Rizatriptan Benzoate

Department of Chemistry, University of Mysore, Manasagangotri, Karnataka, Mysore 570006, India

Received 6 April 2012; Accepted 27 May 2012

Academic Editors: A. Bouklouze, A. Golcu, V. Maier, G. Ramis-Ramos, and A. Szemik-Hojniak

Copyright © 2012 Kudige N. Prashanth and Kanakapura Basavaiah. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Rizatriptan is a new selective 5-HT1B/1D agonist which is used in the treatment of migraine headaches. Two simple, rapid, accurate, and economical spectrophotometric methods are described for the determination of rizatriptan benzoate (RTB) in its pure form and pharmaceutical preparations. These methods are based on the charge-transfer complexation reaction between rizatriptan benzoate as n-electron donor and p-chloranilic acid (p-CA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) as π-acceptor to form highly colored chromogens. The chromogens formed by the reaction between RTB and p-CA peaked at 530 nm (method A) and that formed by the reaction between RTB and DDQ peaked at 590 nm (method B). Under the optimum conditions Beer’s law is obeyed in the concentration range of 14–245 μg mL−1 for method A and 4–70 μg mL−1 for method B. The coefficient of correlation was found to be 0.9999 for both methods. The molar absorptivity, Sandell sensitivity, limits of detection, and quantification are also reported. The stoichiometric relationship determined by Job’s continuous method was found to be 1 : 1 (drug : reagent) for both methods. Both methods were applied to determination of RTB in the pharmaceutical formulations. Results of the analysis were validated statistically.

1. Introduction

Drug quality control is a branch of analytical chemistry that has a wide impact on the health of human being, so the development of a reliable, quick, and accurate method for the active ingredient determination is always welcomed. Of the chemical neurotransmitter substances, serotonin (5-hydroxytriptamine or 5-HT) is perhaps the most implicated in the treatment of migraine, one among them is rizatriptan benzoate (RTB). RTB is a selective 5-hydroxytryptamine1B/1D (5-) receptor agonist which is chemically described as N, N-dimethyl-5-(1H-1,2,4-triazol-1-ylmethyl)-1H-indole-3-ethanamine monobenzoate. It has a weak affinity for other 5-HT receptor subtypes and was launched in 1998 for the acute treatment of migraine in adults [1]. Migraine headache is recognized as a chronic disease with episodic occurrences and is frequently accompanied by gastrointestinal disturbance including nausea and vomiting. The headache may be preceded or accompanied by aura which is characterized by visual disturbances. The effectiveness of triptans, which are serotonin 5- receptor agonist drugs, in these conditions is due to their ability to block the stimulated secretion of neuropeptides from trigeminal nerves to break the nociceptive cycle of migraine. These actions also include constriction of meningeal and cerebral blood vessels [24].

Rizatriptan (RTB) is not official in any pharmacopoeia. The literature survey revealed several reported analytical approaches for the determination of RTB in dosage forms and biological materials including liquid chromatography-electrospray tandem mass spectrometry, LC-MS/MS [9, 10] for human plasma and high performance liquid chromatography with fluorescence detection [11, 12] and in human serum by LC-MS/MS [13]. Development of a rapid, sensitive, and selective method for the determination of RTB is essential for the analysis of drug in bulk, in drug delivery system and for release dissolution studies. A few methods are found in the literature for the determination of RTB in pharmaceuticals and include UV-spectrophotometry [1418], spectrofluorimetry [17], HPLC when present alone [19, 20] or in combination with other antimigraine drugs [21]. A microemulsion electrokinetic chromatography (MEEKC) has also been reported for the determination of RTB and its degradation products [5].

In the literature, only a few visible spectrophotometric methods have been described for the determination of RTB. Shanmukha Kumar et al. [6] have described two methods, one based on the chloroform-soluble ion-pair with methyl orange, and the other, redox-complexation reaction involving iron (III) and 2,2′-bipyridyl. The reaction of RTB with 2,6-dichloroquinone-4-chlorimide, 1,2-naphthaquinone-4-sulphonic acid or brucine and metaperiodate has resulted in colored products serving as basis for the assay of drug [7]. Three more methods have been reported by Shanmukha et al. [8]. The first method is similar to one described earlier involving iron (III) except that 1, 10-phenantroline is used in place of 2,2′-bipyridyl. In the second method, Folin-Ciocalteu (F-C) reagent is reduced by RTB in alkaline medium and the resultant blue chromogen measured. The last method uses alizarin red as the ion-pair reagent to form a chloroform-soluble ion-pair complex with RTB. A method based on the reaction of RTB as n-electron donor with 7,7,8,8-tetracyanoquinodimethane (TCNQ) as a π-acceptor to give highly colored complex species peaking at 570 nm is also found in the literature [22]. Present authors have also been reported a few visible spectrophotometric methods for the quantification of RTB [23, 24].

However, the reported methods, particularly those based on chromatography are complex, require expensive experimental setup, and skilled personnel and inaccessible to many laboratories in developing and underdeveloped nations. In contrast, visible spectrophotometry is considered as the most convenient analytical technique in most quality control and clinical laboratories. Spectrophotometric methods have several advantages, such as low interference level, good analytical selectivity, and they are easier, less expensive, and less time consuming compared with most of the other methods [25]. The reported spectrophotometric methods [68, 22] suffer from one or the other disadvantage such as poor sensitivity, rigid pH control, heating or extraction step, complicated experimental setup, and meticulous control of experimental variables as can be seen from Table 1.

Table 1: Comparison of the proposed and the existing visible spectrophotometric methods.

The aim of this study was to establish simple, sensitive, precise, and inexpensive procedures for the quantification of RTB in pharmaceutical preparations. The basis of the proposed methods is molecular interactions between electron donors and electron acceptors. These interactions are generally associated with the formation of intensely colored charge transfer (CT) complexes which absorb radiation in the visible region [26, 27]. Substituted quinones such as 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), and p-chloranilic acid (p-CA) have been used as π-acceptors with various donors to form CT complexes and radicals, thus enabling the determination of a number of pharmaceutical substances [2835]. This work describes the application of the above reagents (p-CA and DDQ) for the spectrophotometric assay of RTB. The proposed methods have been validated statistically for their accuracy, precision, sensitivity, selectivity, robustness, and ruggedness as per ICH guidelines.

2. Experimental

2.1. Instrument

All absorption measurements were made using a Systronics model 106 digital spectrophotometer (Systronics Ltd, Ahmedabad, India) with 1 cm path length matched quartz cells.

2.2. Materials

Pharmaceutical grade RTB certified to be 99.65% pure was obtained as a gift sample from Jubilant life Sciences, Nanjangud, Mysore, India, and used as received. Rizora-10 and Rizora-5 from Intas pharmaceuticals Ltd., Ahmedabad, India, both tablets were purchased from local commercial sources. 1,4-Dioxane and acetonitrile (spectroscopic grade) were purchased from Merck Specialities Pvt Ltd., Mumbai, India.

2.3. Reagents

p-Chloranilic acid (p-CA) and 2,3-dichloro-5,6-dicyanoquinone (DDQ) both 0.1% solutions (both from S.D. Fine Chem Ltd, Mumbai), were prepared freshly in 1,4-dioxane.

2.4. Standard RTB Stock Solution

For p-CA method, a 350 μg mL−1 RTB stock standard solution was prepared by dissolving 35 mg of pure drug in acetonitrile in a 100 mL volumetric flask and the solution was diluted to the mark with the same solvent; and the above 350 μg mL−1 RTB stock solution was diluted with acetonitrile to get 100 μg mL−1 and used for the assay in DDQ method.

2.5. Construction of Calibration Curves
2.5.1. p-CA Method (Method A)

Varying aliquots of standard RTB solution equivalent to 14.0–245.0 μg mL−1 (0.2–3.5 mL of 350 μg mL−1) were accurately measured and transferred into a series of 5 mL calibrated flasks and the total volume in each flask was brought to 3.5 mL by adding acetonitrile. After the addition of 1 mL of 0.1% p-CA solution, the volume was adjusted to the mark with acetonitrile and mixed well. The absorbance was measured at 530 nm against a reagent blank similarly prepared without adding RTB.

2.5.2. DDQ Method (Method B)

Into a series of 5 mL calibration flasks, aliquots (0.2–3.5 mL) of standard 100 μg mL−1 RTB solution equivalent to 4.0–70.0 μg mL−1 RTB were accurately transferred to 5 mL calibrated flasks, and to each flask, 1 mL of 0.1% DDQ solution was added. The volume was made up to the mark with acetonitrile, mixed well and the absorbance of the red coloured C-T complex was measured at 600 nm against the reference blank similarly prepared.

Standard graphs were prepared by plotting the absorbance versus RTB concentrations, and the concentration of the unknown was read from the calibration graph or computed from the respective regression equation derived using the absorbance-concentration data.

2.5.3. Assay Procedure for Pharmaceutical Preparations

Twenty tablets were weighed and pulverized. An amount of tablet powder equivalent to 35 mg RTB was transferred into a 100 mL volumetric flask and about 70 mL of acetonitrile was added to the flask. The content was shaken well for 20 min and diluted to the mark with the same solvent. The resulting solution was filtered through Whatman No. 42 filter paper and used for the assay by following the general procedure described for p-CA method. The resulting tablet extract (350 μg mL−1) was diluted to 100 μg mL−1 with acetonitrile and suitable aliquot was used for the assay using the general procedure described for DDQ method.

2.5.4. Procedure for the Analysis of Placebo Blank and Synthetic Mixture

A placebo blank containing starch (10 mg), acacia (15 mg), hydroxyl cellulose (10 mg), sodium citrate (10 mg), talc (20 mg), magnesium stearate (15 mg), and sodium alginate (10 mg) was prepared and its solution prepared as described under tablets and then subjected to analysis.

A synthetic mixture was separately prepared by adding pure RTB (50 mg) to the above mentioned placebo blank and the mixture was homogenized. The mixture containing 35 mg of RTB was weighed and its extract was prepared as described for tablets. The extracts containing three different concentrations of RTB were subjected to assay according to the general procedures described earlier and the percentage recovery of RTB was computed.

2.5.5. Stoichiometric Relationship

Job’s method of continuous variations of equimolar solutions was employed to establish the stoichiometry of the colored products. The solutions equivalent to 7.66 × 10−4 and 2.55 × 10−4 M RTB were prepared. Further, 7.66 × 10−4 M p-CA (method A) and 2.55 × 10−4 M DDQ (method B) solutions were prepared in 1,4-dioxane. A series of solutions was prepared in which the total volume of RTB and reagent was kept at 5 mL. The drug and reagent solutions were mixed in various complementary proportions (0 : 5, 1 : 4, 2 : 3,…,5 : 0, inclusive) and made up to mark with acetonitrile. The absorbance of the resulting colored species was measured at 530 nm in method A and 590 nm in method B.

3. Results and Discussion

3.1. Absorption Spectra

The reaction of p-CA as a π-acceptor with rizatriptan benzoate as -electron donor results in the formation of an intense orange-red product which exhibits absorption maxima at 530 nm (Figure 1) due to the formation of the corresponding p-CA radical anion. DDQ also acts as a π-acceptor and the RTB-DDQ charge transfer complex resulted in the formation of an intense reddish violet color which exhibit three maxima at 600, 545, and 455 nm (Figure 2). These bands can be attributed to the formation of DDQ radical anions arising from the complete transfer of n-electrons from donor to acceptor moieties in acetonitrile. The absorption band at 590 nm was selected as analytical wavelength keeping in view the sensitivity of the reaction product and low blank absorbance.

Figure 1: Absorption spectra of charge transfer complex of RTB-p-CA (140 μg mL−1 RTB): (1) p-CA radical anion (2) p-CA in acetonitrile.
Figure 2: Absorption spectra of charge transfer complex of RTB-DDQ (40 μg mL−1 RTB): (a) DDQ radical anion (b) DDQ in acetonitrile.

3.2. Reaction Mechanism

The chemistry involved in the proposed methods is based on the reaction of the basic nitrogen (The more basic secondary amine) of RTB as n-donor with the π-acceptors, namely, p-CA and DDQ to form charge transfer complexes which subsequently dissociate into radical anions depending on the polarity of the solvent used. In polar solvents, such as acetonitrile, complete electron transfer from the donor to the acceptor moiety takes place with the formation of intensely colored radical anions [36], according to the following equation:

The dissociation of the complex is promoted by the high ionizing power of the acetonitrile. The tentative reaction mechanisms for RTB-p-CA and RTB-DDQ complexes are proposed and illustrated in Schemes 1 and 2, respectively.

Scheme 1: The tentative reaction mechanism for RTB-p-CA complex.
Scheme 2: The tentative reaction mechanism for RTB-DDQ complex.
3.3. The Effect of Different Experimental Variables
3.3.1. Effect of Reagent Concentration

The effect of the reagent concentration on the intensity of the color developed at the selected wavelengths was ascertained by adding different amounts of the reagents p-CA and DDQ to fixed concentrations of 150 and 40 μg mL−1 RTB in method A and method B, respectively. It was found that 1.0 mL of 0.1% p-CA and 1.0 mL of 0.1% DDQ solutions were sufficient for the production of maximum and reproducible color intensity and the sensitivity decreases by further addition of these reagents (Figure 3). Higher volumes are undesirable since they resulted in increasing blank absorbances.

Figure 3: Effect of reagent concentration on color development: (a) RTB (150 μg mL−1) and p-CA (0.1%); (b) RTB (40 μg mL−1) and DDQ (0.1%).
3.3.2. Effect of Solvent

To dissolve RTB, acetonitrile was preferred to chloroform, dichloromethane, acetone, 2-propanol, dichloroethane, 1,4-dioxane, methanol or ethanol, because the complex formed in these solvents either had very low absorbance values or precipitated upon dilution. Whereas in the case of reagents, highly intense colored products were formed when 1,4-dioxane medium was used as a solvent to dissolve p-CA and DDQ. Therefore, acetonitrile and 1,4-dioxane were chosen as solvents to dissolve RTB and the reagents, respectively. Similarly, the effect of the diluting solvent was studied and the results showed that none of the solvents except acetonitrile favored sensitive and stable colored species in both methods. Thus, acetonitrile was used for dilution throughout the investigation.

3.3.3. Effect of Reaction Time

The optimum reaction time was determined by following the color development upon the addition of p-CA and DDQ solutions to the RTB solution at room temperature. Complete color development was attained instantaneously with both the reagents. The absorbance of these radical anions remained stable for at least 3 hrs and 2 hrs for method A and method B, respectively.

3.3.4. Molar Ratio of the Reaction

Job’s continuous variations graph for the reaction between RTB and p-CA or DDQ (Figure 4) shows that the interaction occurs on an equimolar basis via the formation of a charge transfer complexes 1 : 1 (RTB: reagent). This finding was anticipated by the presence of more basic or electron donating centre (–NH) in the RTB.

Figure 4: Job’s Continuous-variation plot: (a) [RTB] and [p-CA] = 7.66 ×10−4 M (b) [RTB] and [DDQ] = 2.55 × 10−4 M.
3.4. Method Validation
3.4.1. Linearity

Under optimum experimental conditions for determination of the drug under study, the absorbance versus concentration plots were found to be linear over the concentration ranges stated in Table 2. The regression parameters calculated from the calibration graphs data, along with the standard deviations of the slope () and the intercept () are presented in Table 2. The linearity of the calibration graphs was demonstrated by the high values of the correlation coefficient (r) and the small values of the y-intercepts of the regression equations. The calculated molar absorptivity, Sandell sensitivity values of the methods A and B were also cited in Table 2.

Table 2: Regression and analytical parameters.
3.4.2. Limit of Detection (LOD) and Limit of Quantification (LOQ)

The LOD for the proposed methods were calculated using the equation [35]: where, “” is the standard deviation of replicate determination values under the same conditions as for the sample analysis in the absence of the analyte and “” is the sensitivity, namely, the slope of the calibration graph.

The LOQ, defined as [36]: Based on the above equations, the limits of detection and quantification were calculated and recorded in Table 2.

3.4.3. Assay Precision and Accuracy

In order to determine the precision of the proposed methods, solutions containing three different concentrations of RTB were prepared and analyzed in five replicates and the analytical results are summarized in Table 3. The low values of the percentage relative standard deviation (% R.S.D) and percentage relative error (% R.E) indicate the high precision and the good accuracy of the proposed methods. RSD (%) and RE (%) values were obtained within the same day to evaluate repeatability (intra day precision) and over five days to evaluate intermediate precision (inter day precision).

Table 3: Evaluation of intraday and interday precision and accuracy.
3.4.4. Robustness and Ruggedness

The robustness of the methods was evaluated by making small incremental changes in the volume of dye ( mL) and the effect of the change was studied on the absorbance of the C-T complex. The changes had negligible influence on the results as revealed by small intermediate precision values expressed as % RSD (≤1.28%). Method ruggedness was demonstrated having the analysis done by four analysts, and also by a single analyst performing analysis using four different cuvettes. Intermediate precision values (%RSD) in both instances were in the range 0.99–1.74 indicating acceptable ruggedness. These results are presented in Table 4.

Table 4: Robustness and ruggedness.
3.4.5. Selectivity

In order to evaluate the selectivity of the proposed methods for the analysis of pharmaceutical formulations, the effect of the presence of common excipients, such as talc, starch, acacia, hydroxyl cellulose, sodium citrate, magnesium stearate, and sodium alginate was tested for possible interference in the assay by placebo blank and synthetic mixture analyses.

The analysis of synthetic mixture solution prepared as described earlier yielded percent recoveries which ranged between 99.02–102.1 and with standard deviation of 0.79–1.94 (). The results of this study showed that the inactive ingredients did not interfere in the assay indicating the high selectivity of the proposed method.

3.4.6. Applications to Analysis of Pharmaceutical Formulations

The proposed methods were successfully applied to the determination of RTB in two representative tablets Rizora-10 and Rizora-5. The results obtained are showed in Table 5 and were compared with those obtained by the reference method [17] by means of Student’s t- and F-tests at 95% confidence level. The reference method consisted of the measurement of the absorbance of the tablet extract in water at 225 nm. In all cases, the average results obtained by the proposed methods and reference method were statistically identical, as the difference between the average values had no significance at 95% confidence level with respect to accuracy and precision, as demonstrated by the Student’s t- and F- tests.

Table 5: Results of analysis of tablets by the proposed methods.
3.4.7. Recovery Study

To ascertain the accuracy and validity of the proposed methods, recovery experiment was performed via standard addition technique. To a fixed and known amount of RTB in tablet powder (preanalyzed), pure RTB was added at three concentration levels (50, 100, and 150% of the level present in the tablet) and the total was measured by the proposed methods. The determination with each concentration was repeated three times and the results of this study presented in Table 6 indicated that the various excipients present in the formulations did not interfere in the assay.

Table 6: Results of recovery study by standard addition method.

4. Conclusions

The methods are based on well-characterized charge-transfer complexation reaction, and have the advantages of simplicity, speed, accuracy and precision, and use of inexpensive equipment compared to the reported HPLC and LC-MS methods. Other advantage of these methods is wide linear range. The DDQ method is more sensitive than the p-CA method as seen from the higher molar absorptivity value. Moreover, the proposed methods can be performed at room temperature. Thus, the methods are useful for the quality control and routine analysis of RTB in pharmaceuticals since there is no interference from the common excipients that might be found in commercial formulations.


The authors wish to acknowledge Cipla India Ltd., Mumbai, India, for providing the gift sample of atenolol. One of the authors (K. N. Prashanth) also wishes to thank the authorities of the University of Mysore for giving permission and facilities to carry out the research work.


  1. P. J. Goadsby, “Serotonin 5-HT1B/1D receptor agonists in migraine: comparative pharmacology and its therapeutic implications,” CNS Drugs, vol. 10, no. 4, pp. 271–286, 1998. View at Google Scholar · View at Scopus
  2. S. Gori, N. Morelli, G. Bellini et al., “Rizatriptan does not change cerebral blood flow velocity during migraine attacks,” Brain Research Bulletin, vol. 65, no. 4, pp. 297–300, 2005. View at Publisher · View at Google Scholar · View at Scopus
  3. A. D. Oldman, L. A. Smith, H. J. McQuay, and R. A. Moore, “Pharmacological treatments for acute migraine: quantitative systematic review,” Pain, vol. 97, no. 3, pp. 247–257, 2002. View at Publisher · View at Google Scholar · View at Scopus
  4. D. J. Williamson, S. L. Shepheard, R. G. Hill, and R. J. Hargreaves, “The novel anti-migraine agent rizatriptan inhibits neurogenic dural vasodilation and extravasation,” European Journal of Pharmacology, vol. 328, no. 1, pp. 61–64, 1997. View at Publisher · View at Google Scholar · View at Scopus
  5. P. E. Mahuzier, B. J. Clark, A. J. Crumpton, and A. J. Kevin, “Quantitative microemulsion electrokinetic capillary chromatography analysis of formulated drug products,” Journal of Separation Sciences, vol. 24, no. 9, pp. 784–788, 2001. View at Google Scholar
  6. J. V. Shanmukha kumar, D. Ramachandran, V. S. Settaluri, L. A. Lakshmi, and F. C. Shechinah, “Validation of analytical procedures for determination of rizatriptan benzoate,” The Pharma Research, vol. 4, pp. 28–37, 2010. View at Google Scholar
  7. S. D. Gowry and K. M. Vamsi, “Visible spectrophotometric methods for the determination of rizatriptan in pure form and in pharmaceutical formulations,” Analytical Chemistry, vol. 3, pp. 4–6, 2007. View at Google Scholar
  8. K. N. Prashanth and K. Basavaiah, “Sensitive and selective methods for the determination of rizatriptanbenzoate in pharmaceuticals using N-bromosuccinimide and two dyes,” Journal of Saudi Chemical Society. In press.
  9. J. F. Guo, A. J. Zhang, L. Zhao et al., “Determination of rizatriptan in human plasma by liquid chromatographic-eletrospray tandem mass spectrometry: application to a pharmacokinetic study,” Biomedical Chromatography, vol. 20, no. 1, pp. 61–66, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. Y. Chen, H. Miao, M. Lin et al., “Development and validation of a selective and robust LC-MS/MS method for high-throughput quantifying rizatriptan in small plasma samples: application to a clinical pharmacokinetic study,” Journal of Chromatography B, vol. 844, no. 2, pp. 268–277, 2006. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. P. Qin, Y. G. Zou, M. Z. Liang, and Q. Yu, “Determination of rizatriptan in human plasma by RP-HPLC with fluorescence detection,” Yaowu Fenxi Zazhi, vol. 26, no. 1, pp. 7–9, 2006. View at Google Scholar
  12. J. Chen, X. Jiang, W. Jiang, N. Mei, X. Gao, and Q. Zhang, “Liquid chromatographic method for the determination of rizatriptan in human plasma,” Journal of Chromatography B, vol. 805, no. 1, pp. 169–173, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. K. Vishwanathan, M. G. Bartlett, and J. T. Stewart, “Determination of antimigraine compounds rizatriptan, zolmitriptan, naratriptan and sumatriptan in human serum by liquid chromatography/electrospray tandem mass spectrometry,” Rapid Communicatons in Mass Spectrometry, vol. 14, no. 3, pp. 168–172, 2000. View at Google Scholar
  14. K. Amol, R. Vivek, K. Alpana, D. M. Hassan, S. Maria, and L. Swaroop, “Spectrophotometric method for analysis of rizatriptan benzoate,” International Journal of Pharmaceutical Sciences, vol. 1, no. 2, pp. 307–309, 2009. View at Google Scholar
  15. A. S. Kumari, S. Subhasish, D. K. Kaushik, and M. M. Annapurna, “UV-spectroscopic methods for estimation of rizatriptan benzoate in pharmaceutical preparations,” International Journal of ChemTech Research, vol. 2, no. 1, pp. 653–659, 2010. View at Google Scholar · View at Scopus
  16. R. Vivek, K. Amol, K. Alpana, D. M. Hassan, and D. M. Maria, “Spectrophotometric estimation of rizatriptan benzoate,” Asian Journal of Research in Chemistry, vol. 3, no. 1, pp. 175–177, 2010. View at Google Scholar
  17. S. Altinoz, G. Ucar, and E. Yildiz, “Determination of rizatriptan in its tablet dosage forms by UV spectrophotometric and spectrofluorimetric methods,” Analytical Letters, vol. 35, no. 15, pp. 2471–2485, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. M. M. Annapurna, S. Sravya, and C. Vineesha, “New derivative spectrophotometric methods for the determination of rizatriptan benzoate in pharmaceutical dosage forms,” International Journal of Pharmaceutical Sciences Review and Research, vol. 13, no. 2, pp. 133–136, 2012. View at Google Scholar
  19. M. Zečević, B. Jocić, L. Živanović, and A. Protić, “Application of multicriteria methodology in the development of improved RP-LC-DAD for determination of rizatriptan and its degradation products,” Chromatographia, vol. 68, no. 11-12, pp. 911–918, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. B. Jocić, M. Zečević, L. Živanović, and A. Ličanski, “A chemometrical approach to optimization and validation of an HPLC assay for rizatriptan and its impurities in tablets,” Analytical Letters, vol. 40, no. 12, pp. 2301–2316, 2007. View at Publisher · View at Google Scholar · View at Scopus
  21. P. V. Sagar, D. Kumar, and D. Suddhasattya, “Simultaneous estimation of rizatriptan, sumatriptan and zolmitriptan by RP-HPLC method in bulk,” Journal of Pharmacy Research, vol. 3, no. 12, pp. 2930–2933, 2010. View at Google Scholar
  22. K. N. Prashanth, K. Basavaiah, and K. B. Vinay, “Sensitive and selective spectrophotometric assay of rizatriptanbenzoate in pharmaceuticals using three sulphonphthalein dyes,” Arabian Journal of Chemistry. In press.
  23. J. V. Shanmukha Kumar, K. R. S. Prasad, D. Ramachandran, and V. S. Settaluri, “Development and validation of spectrophotometric methods for determination of maxalt (Rizatriptan Benzoate) in pure and pharmaceutical formulation,” IJPI’s Journal of Analytical Chemistry, vol. 1, pp. 1–5, 2011. View at Google Scholar
  24. I. E. Ramzia, G. M. Nashwah, and A. Heba, “Fluorimetric and colorimetric methods for the determination of some antimigraine drugs,” Journal of Chemical and Pharmaceutical Research, vol. 3, no. 4, pp. 304–314, 2011. View at Google Scholar · View at Scopus
  25. C. Saka, “Review of analytical methods for identification and determination of triptans,” Critical Reviews in Analytical Chemistry, vol. 39, no. 1, pp. 32–42, 2009. View at Publisher · View at Google Scholar · View at Scopus
  26. R. S. Mulliken, “Structures of complexes formed by halogen molecules with aromatic and with oxygenated solvents,” Journal of the American Chemical Society, vol. 72, no. 1, pp. 600–608, 1950. View at Google Scholar · View at Scopus
  27. R. Foster, Organic Charge-Transfer Complexes, Academic Press, New York, NY, USA, 1969.
  28. E. Khaled, “Spectrophotometric determination of terfenadine in pharmaceutical preparations by charge-transfer reactions,” Talanta, vol. 75, no. 5, pp. 1167–1174, 2008. View at Publisher · View at Google Scholar · View at Scopus
  29. M. X. Cijo, K. Basavaiah, S. A. M. Abdulrahman, and K. B. Vinay, “Spectrophotometric determination of repaglinide in tablets based on charge-transfer complexation reaction with chloranilic acid and dichloro-dicyano benzoquinone,” Chemical Industry & Chemical Engineering Quarterly, vol. 17, no. 4, pp. 469–476, 2011. View at Google Scholar
  30. K. B. Vinay, H. D. Revanasiddappa, M. S. Raghu, A. M. Sameer. Abdulrahman, and N. Rajendraprasad, “Spectrophotometric determination of mycophenolate mofetil as its charge-transfer complexes with two π-acceptors,” Journal of Analytical Methods in Chemistry, vol. 2012, Article ID 875942, 8 pages, 2012. View at Publisher · View at Google Scholar
  31. K. Basavaiah, K. Tharpa, and K. B. Vinay, “Spectrophotometric determination of isoxsuprine hydrochloride as base form in pharmaceutical formulation through charge transfer complexation,” Croatica Chemica Acta, vol. 83, no. 4, pp. 415–420, 2010. View at Google Scholar · View at Scopus
  32. M. Pandeeswaran and K. P. Elango, “Spectroscopic and kinetic studies on the interaction of ketoconazole and povidone drugs with DDQ,” Spectrochimica Acta A, vol. 69, no. 4, pp. 1082–1088, 2008. View at Publisher · View at Google Scholar · View at Scopus
  33. K. Basavaiah and S. A. M. Abdulrahman, “Use of charge transfer complexation reaction for the spectrophotometric determination of bupropion in pharmaceuticals and spiked human urine,” Thai Journal of Pharmaceutical Sciences, vol. 34, no. 4, pp. 134–145, 2010. View at Google Scholar · View at Scopus
  34. M. E. Abdel-Hamid, M. Abdel-Salam, M. S. Mahrous, and M. M. Abdel-Khalek, “Utility of 2,3-dichloro-5,6-dicyano-p-benzoquinone in assay of codeine, emetine and pilocarpine,” Talanta, vol. 32, no. 10, pp. 1002–1004, 1985. View at Google Scholar · View at Scopus
  35. R. Nafisur and K. Mohammad, “Optimized and validated spectrophotometric methods for the determination of roxatidine acetate hydrochloride in drug formulations using 2,3-dichloro-5,6-dicyano-1,4-benzoquinone and p-chloranilic acid,” Journal of Analytical Chemistry, vol. 60, no. 7, pp. 636–643, 2005. View at Publisher · View at Google Scholar · View at Scopus
  36. ICH Harmonised Tripartite Guideline, “Validation of analytical procedures: text and methodology Q2(R1),” in Proceedings of the International Conference on Hormonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Complementary Guideline on Methodology, London, UK, November 1996, Incorporated in November 2005.