Comparison of CD63 Upregulation Induced by NSAIDs on Basophils and Monocytes in Patients with NSAID Hypersensitivity

Table 5

Activation of basophils (BAT), monocytes (MAT), and neutrophils (NAT) induced in vitro by ASA (1 mg/mL) or diclofenac (0.125 mg/mL).

		Patients with positive tests*
Group of patients		BAT+		MAT+		NAT+
		ASA	Diclofenac	ASA	Diclofenac	ASA	Diclofenac
NSAID HS	𝑛=60	22 (37%)	20 (33%)	14 (23%)	15 (25%)	8 (13%)	16 (27%)
Grade I**	𝑛=38	8 (21%)	10 (26%)	4 (11%)	9 (24%)	6 (16%)	12 (31%)
Grade II	𝑛=22	14 (64%)	10 (46%)	10 (46%)	6 (27%)	2 (9%)	4 (19%)
Controls	𝑛=20	2 (10%)	5 (25%)	5 (25%)	3 (15%)	0 (0%)	2 (10%)

*The optimal cut-off point for a positive test deduced from ROC curves was 6% of activated cells (see Section 3). Activation was detected by CD63 upregulation. **Grading of reactions according to severity of clinical symptoms (Tables 2 and 4).