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Journal of Amino Acids
Volume 2012 (2012), Article ID 415713, 1 page

Erratum to “Decreased Phosphorylation and Increased Methionine Oxidation of -Synuclein in the Methionine Sulfoxide Reductase A Knockout Mouse”

Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence, KS 66045, USA

Received 15 November 2012; Accepted 6 December 2012

Copyright © 2012 Derek B. Oien et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

On page 4 of this published article an error in Figure 2(a) has occurred. Accordingly, Figure 2(a) has been replaced with a corrected version of the figure as shown below.

Figure 2: Phosphorylation of α-synuclein in and wild-type (WT) brain extracts. (a) Tris-soluble and Urea-soluble brain extracts (40 μg protein) of both mouse types were prepared as described in Section 2. These extracts were then incubated in the presence of additional brain-matched Tris-soluble extract (10 μg protein, serving as a source for kinases), 25 mM Tris (pH 7.4), protease inhibitor cocktail (no-EDTA) (Roche), 1 mM CaCl2, 10 mM MgCl2, and 16.7 μM [γ- ]-ATP for 3 minutes at room temperature in a final volume of 50 μL. Endogenous phosphorylation was stopped by addition of 10 mM EDTA, 10 mM EGTA, 1 mM cold ATP and was immediately placed on ice. Then, the samples were subjected to an immunoprecipitation by anti-α-synuclein antibodies or anti-MetO antibodies as described in Section 2. Thereafter, equal protein amounts of the immunoprecipitants were subjected to an SDS-gel electrophoresis (4–20%) followed by exposure of the gel to an X-ray film. Lanes 1, 3, 5, and 7 represent Tris-soluble fractions, and lanes 2, 4, 6, and 8 represent urea-soluble fractions. Syn: α-synuclein; ab: antibodies; kDA: molecular mass markers in kilo-Dalton. The detected band following the immunoprecipitation by anti-MetO antibodies was also denoted in the text as MetO-15.