- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Article Processing Charges ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Recently Accepted Articles ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Journal of Amino Acids
Volume 2013 (2013), Article ID 646703, 7 pages
Acute Effects of an Energy Drink on Myocardial Function Assessed by Conventional Echo-Doppler Analysis and by Speckle Tracking Echocardiography on Young Healthy Subjects
Department of Cardiovascular Diseases, University of Siena, Viale Bracci, 53100 Siena, Italy
Received 24 June 2013; Revised 6 September 2013; Accepted 8 September 2013
Academic Editor: Hari S. Sharma
Copyright © 2013 Daniele Menci et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Purpose. Previous studies have underlined the effects of the energy drinks containing caffeine end taurine on the cardiovascular system. The aim of this study was to determine acute changes on echocardiographic parameters assessed by conventional echo-Doppler analysis and by speckle tracking echocardiography after the consumption of an energy drink in a young healthy population. Methods. measurement of blood pressure, electrocardiographic, and echocardiographic examination were performed on 35 healthy subjects (mean age 25 ± 2 years, 16 men), at baseline and one hour after the consumption of a body surface area indexed amount of an energy drink (168 mL/m2) containing caffeine (0.03%) and taurine (0.4%). Results. The analysis of left ventricular function showed a significant increase of mean relative values of MAPSE (+11%; ), global longitudinal strain (+10%, ), and left ventricular twisting (+22%, ) in respect to baseline. Also, right ventricular function parameters appeared significantly increased after energy drink consumption, as TAPSE (+15%, ), global, and free wall right ventricular longitudinal strain (+8%, ; +5%, , resp.). Conclusion. In conclusion, the consumption of the ED in our population showed a significant increase of right and left ventricular myocardial function, suggesting a possible positive inotropic effect related to the substances contained therein.
Energy drinks (ED) have increased in popularity among adolescents and young adults, because of the possible ergogenic effects [1–4] and the improvement of cognitive performance due to some ingredients found in these beverages [5, 6], in particular caffeine, taurine, glucuronolactone, and glucose. So far, little evidence exists regarding acute effects of the ED on cardiac function. The most important cardiovascular effects of caffeine in acute settings are the increase of blood pressure and circulating concentration of norepinephrine, the increase of arterial stiffness, and the impairment of endothelium-dependent vasodilation .
Taurine (aminoethane sulfonic acid) is a ubiquitous compound found in very high concentrations in heart and muscle. It has been demonstrated that it has a role in the control mechanism of myocardial contractility and studies in animal models have shown that the lack of taurine induces the onset of dilated cardiomyopathy; the beneficial effect of taurine on heart failure was also reported . Moreover, it has been demonstrated that the use of both taurine and caffeine is able to determine a reduction of the effects of caffeine, especially concerning heart rate modifications . Regarding the cardiac effects, an echocardiographic study, concerning the influence of a drink containing taurine and caffeine, performed on healthy subjects, has demonstrated an improvement of left ventricular contractility about forty minutes after taking it .
The aim of this study was to investigate whether taking a taurine and caffeine containing energy drink determines acute changes in myocardial function assessed by conventional echo-Doppler analysis and by speckle tracking echocardiography (STE), a new technique for assessing myocardial function .
2. Materials and Methods
2.1. Study Population
The study group included 35 healthy young volunteers (mean age years, 16 men). All subjects had unremarkable history and normal findings at physical examination, electrocardiogram, and echocardiography. None of them was taking any drugs. Ten of them were smokers. Eight subjects were usual consumers of energy drinks (no more than a can a week). Only 7 of them practiced sports. Characteristics of the study population are reported in Table 1.
2.2. Study Protocol
All participants were asked to abstain from smoking, coffee, and other food or beverages containing caffeine for at least 12 hours before the examinations. All the examinations were carried out in the afternoon between meals. Baseline clinical, blood pressure, ECG, and echocardiographic measurements were made after 5 minutes of supine rest in a quiet and comfortable environment. After baseline examination, all subjects drank 168 mL/m2 (BSA, Gehan & George) of an energy drink containing caffeine (0.03%), taurine (0.4%), glucuronolactone (0.24%), glucose, and other ingredients, in maximum 5 minutes, and they underwent again echocardiography, electrocardiography, and blood pressure measurements 1 hour after drinking. Each participant was also studied in a control experiment by an equal volume of fruit juice one day after energy drink consumption.
The analysis of files recorded was performed offline by a single, experienced, and independent echocardiographer, who did not know if the images refer to those obtained at baseline, after energy drink, or fruit juice consumption, using a commercially available, semiautomated, 2-dimensional strain software (EchoPac, GE, Milwaukee, WI, USA). The study protocol was in accordance with the Helsinki Declaration and the ethical standards of our institution, and all participants gave informed consent for participation in the study.
2.3. Standard Echocardiography
Echocardiographic studies were performed using a high-quality ultrasound machine (Vivid 7; GE, Milwaukee, WI) with the subjects in the left lateral recumbent position. All measurements were made in accordance with current recommendations of American Society of Echocardiography (ASE) .
Left ventricle (LV) systolic function was analyzed by calculating left ventricle ejection fraction (LVEF), measured using Simpson’s method, and by obtaining left ventricle longitudinal function parameters, as mitral annular systolic plane systolic excursion (MAPSE) with M-mode and mean peak systolic annular velocity with pulsed tissue-Doppler (mitral ) by averaging values measured at septal and lateral mitral annulus . Left ventricle diastolic function was assessed by the ratio between peak early () and late diastolic () LV filling velocities with trans-mitral pulsed Doppler and by mean and mitral velocity with pulsed tissue Doppler . ratio was also calculated .
Right ventricle (RV) systolic function was assessed by calculating tricuspid annular plane systolic excursion (TAPSE) and mean peak systolic annular velocity by pulsed tissue Doppler on tricuspid annulus (tricuspid ).
2.4. Speckle-Tracking Echocardiography
The speckle-tracking analysis was performed in accordance with the indications of the ASE/EAE consensus document of Mor-Avi et al. . Apical 4-chamber, 2-chamber, apical long-axis view, basal, and apical short axis view images were obtained using conventional 2-dimensional grayscale echocardiography, during breath hold and with a stable ECG trace. Three consecutive heart cycles were recorded and averaged. The frame rate was set between 60 and 80 frames per second. From apical views, we calculated left ventricular longitudinal strain (GLS), free wall and global right ventricular longitudinal strain (RVLS) , left atrial (LA), and right atrial (RA) longitudinal strain [18, 19]. For the Twisting analysis, after manual demarcation of LV endocardium by a point-and-click approach, at the basal and apical short-axis views, rotation-time curves were constructed for each segment. The global basal and apical rotations were estimated as the average angular displacement of 6 myocardial segments during systole. Rotation angles were expressed in degrees. LV twisting curve was automatically generated as the net difference between mean apical and basal rotation [20, 21].
2.5. Statistical Analysis
Data are reported as mean ± SD. Changes in the variables observed after ED and fruit juice consumption were compared using the Student’s -test for paired data. A value <0.05 was considered statistically significant. Analyses were performed using the SPSS (Statistical Package for the Social Sciences, Chicago, IL) software release 11.5.
All the variables at baseline and after taking ED are shown in Table 2. Figure 1 shows the mean relative increases of parameters studied at baseline, after energy drink, and in the control challenges. Significant variations occurred on LV myocardial deformation parameters after taking the energy drink (Figure 2). Mean relative increases of MAPSE, GLS, and twisting by STE were 11%, 10%, and 22% (Figure 1). All these variables had a very significant increase in respect to baseline with a value of <0.001, 0.004, and <0.0001, respectively (Table 2). Mitral did not show significant modification. LVEF showed an enhanced global LV systolic function with an increase of 5% () from baseline. The parameters of RV deformation underwent significant changes: TAPSE, global RVLS, and free wall RVLS had a mean relative increase of 15% (), 8% (), and 5% (), respectively (Table 2, Figure 3). Tricuspidal did not show any modification. LA and RA deformation assessed by global peak of atrial longitudinal strain (PALS) did not undergo significant variations in respect to baseline. There were no significant changes in either Doppler parameters. Blood pressure and electrocardiographic parameters were showen to be roughly the same between groups, and a mild increase of 6% in diastolic blood pressure after ED consumption was not significant (). There were no significant changes in the parameters measured at baseline and after taking the juice, as shown in Table 3.
In addition to standard echo-Doppler analysis, speckle tracking echocardiography (STE) was used in our study to assess cardiac deformation in three spatial directions: longitudinal, radial, and circumferential. STE is a new technique for assessing myocardial function in physiological and pathological settings, and its feasibility and accuracy were tested in comparison with tagged magnetic resonance imaging, the gold standard to study myocardial deformation. It has been proved that STE is able to detect initial ventricular dysfunction in hypertension, diabetes, valvular heart disease, and heart failure, with high sensitivity in analyzing minimal change in myocardial deformation [11, 16].
In our study population of 35 young healthy subjects, taking an ED containing sugar, caffeine (0.03%), and taurine (0.4%) showed a significant change of myocardial function of both LV and RV one hour after drinking it, suggesting a possible positive effect on cardiac inotropism.
In fact, the study of LV performance showed an increase of longitudinal function, with an increase of MAPSE and GLS, and a remarkable enhancement of LV Twisting (Figure 2). These modifications can probably explain the concomitant increase of LV global function represented by LVEF.
Likewise, the study of RV performance showed an improvement of longitudinal function with a significant increase of TAPSE, and global and free RVLS in respect to baseline (Figure 3). Conversely, no significant changes in LA and RA function were found. Mitral and tricuspid plane excursion underwent a major change in respect to LV and RV longitudinal strain despite the higher sensitivity of the latter, but M-mode analysis only evaluates the displacement of the basal portion of the ventricles, unlike STE analysis that indicates the average of the displacements of all ventricular segments .
Such modifications on cardiac mechanics are probably due to the inotropic effect of some substances contained in the ED.
In particular, taurine seems to have a similar digital effect by means of the control of myocardial contractility, modulating sarcoplasmic reticular Ca2+ release, and stimulating the pumping rate of Ca2+ activate ATPase pumps , preventing intracellular Ca2+ overload.
In fact, it has been reported that taurine may reduce left ventricular end-diastolic pressure in patients with heart failure . Furthermore, in Japan, taurine has been used clinically and has been claimed to be beneficial to patients that are unresponsive or resistant to digitalis or diuretics . Also, the effect of caffeine could be involved in producing an increase in the myocardial contraction, but previous studies have ruled out a positive inotropic effect of caffeine on myocardial cells [24, 25]. An inotropic effect due to some substances of this ED can also explain the increase of LV twist after taking the drink. In fact, the effect of contractility on torsion has been well demonstrated; positive inotropic interventions, such as dobutamine infusion and paired pacing, greatly increase torsion [26–28], especially through augmentation of left ventricular apical endocardial rotation .
In normal subjects, LV twist represents a mechanism for generating stored energy during systole, which is released during early diastole to produce ventricular recoil, upward annular motion, and suction, confirming the close relation of systolic function to early diastole . In normal hearts, all of these aspects of ventricular function increase on exercise to aid fast ejection and, more important, enable rapid filling of the ventricle during a shortened diastole period while maintaining a low filling pressure .
The LV twisting motion is a consequence of myocardial fiber orientation, which changes from an approximately longitudinal but slightly oblique orientation in the subendocardium to a circumferential orientation in the mid-wall and to an oblique orientation in the subepicardium [32, 33]. Thus, the subendocardial and subepicardial fibers represent two oppositely directed spirals and in normal heart because of larger radii, the torque of subepicardial fibers dominates over subendocardial fibers and accounts for the normal counterclockwise rotation of the LV apex.
Previous studies have found that in the setting of LV diastolic dysfunction, torsion is increased in patients with mild diastolic dysfunction but reduced in those with more severe degrees of diastolic dysfunction ; these changes may be explained by the fact that in the early stage, the subendocardial longitudinal fibers are primarily affected so that unopposed epicardial torque will increase apical twist, whereas later more widespread fibrosis or damage will affect global function and lead to reduced twist.
Moreover, Tan et al.  showed that in patients affected by heart failure with preserved ejection fraction, in contrast with normal subjects, these abnormalities of LV function became more apparent on exercise, demonstrating a reduction of myocardial systolic strain, longitudinal function, and finally twisting motion.
Since this study was performed on young healthy individuals at rest, future studies need to focus on whether such benefits persist after long term consumption of energy drinks and what the effects are of consuming these drinks during physical activity; it will also be important to determine which of the effects are induced in patients with cardiac disease to further our understanding of the potential benefits or risks of energy drink consumption.
4.1. Study Limitations
Some limitations should be considered in this study. We evaluated young healthy subjects, so these results cannot be generalized for other populations. The number of subjects of our sample was limited, although it has allowed us to reach interesting conclusions. Moreover, we have investigated the acute changes on myocardial performance only one hour after energy drink consumption. Based on the pharmacokinetics of certain substances of this drink (such as caffeine and taurine), it should be interesting to evaluate the cardiovascular effect at different times from the consumption of these beverages. Lastly, we have used a pear juice as a control, but the same volume of this beverage does not correspond to the same amount of sugar dissolved in the energy drink.
- C. Alford, H. Cox, and R. Wescott, “The effects of red bull energy drink on human performance and mood,” Amino Acids, vol. 21, no. 2, pp. 139–150, 2001.
- J. L. Ivy, L. Kammer, Z. Ding et al., “Improved cycling time-trial performance after ingestion of a caffeine energy drink,” International Journal of Sport Nutrition and Exercise Metabolism, vol. 19, no. 1, pp. 61–78, 2009.
- S. L. Ballard, J. J. Wellborn-Kim, and K. A. Clauson, “Effects of commercial energy drink consumption on athletic performance and body composition,” Physician and Sportsmedicine, vol. 38, no. 1, pp. 107–117, 2010.
- C. M. Lockwood, J. R. Moon, A. E. Smith et al., “Low-calorie energy drink improves physiological response to exercise in previously sedentary men: a placebo-controlled efficacy and safety study,” Journal of Strength and Conditioning Research, vol. 24, no. 8, pp. 2227–2238, 2010.
- E. Duchan, N. D. Patel, and C. Feucht, “Energy drinks: a review of use and safety for athletes,” Physician and Sportsmedicine, vol. 38, no. 2, pp. 171–179, 2010.
- F. van den Eynde, P. C. van Baelen, M. Portzky, and K. Audenaert, “Energy drink effects on cognitive performance,” Tijdschrift voor Psychiatrie, vol. 50, no. 5, pp. 273–281, 2008.
- N. P. Riksen, G. A. Rongen, and P. Smits, “Acute and long-term cardiovascular effects of coffee: implications for coronary heart disease,” Pharmacology and Therapeutics, vol. 121, no. 2, pp. 185–191, 2009.
- S. W. Schaffer, C. Ju Jong, R. Kc, and J. Azuma, “Physiological roles of taurine in heart and muscle,” Journal of Biomedical Science, vol. 17, supplement 1, article S2, 2010.
- A. Bichler, A. Swenson, and M. A. Harris, “A combination of caffeine and taurine has no effect on short term memory but induces changes in heart rate and mean arterial blood pressure,” Amino Acids, vol. 31, no. 4, pp. 471–476, 2006.
- M. Baum and M. Weib, “The influence of a taurine containing drink on cardiac parameters before and after exercise measured by echocardiography,” Amino Acids, vol. 20, no. 1, pp. 75–82, 2001.
- S. Mondillo, M. Galderisi, D. Mele et al., “Speckle-tracking echocardiography: a new technique for assessing myocardial function,” Journal of Ultrasound in Medicine, vol. 30, no. 1, pp. 71–83, 2011.
- R. M. Lang, M. Bierig, R. B. Devereux et al., “Recommendations for chamber quantification: a report from the American Society of Echocardiography's guidelines and standards committee and the Chamber Quantification Writing Group, developed in conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology,” Journal of the American Society of Echocardiography, vol. 18, no. 12, pp. 1440–1463, 2005.
- V. Zacà, P. Ballo, M. Galderisi, and S. Mondillo, “Echocardiography in the assessment of left ventricular longitudinal systolic function: current methodology and clinical applications,” Heart Failure Reviews, vol. 15, no. 1, pp. 23–37, 2010.
- M. Kasner, D. Westermann, P. Steendijk et al., “Utility of Doppler echocardiography and tissue Doppler imaging in the estimation of diastolic function in heart failure with normal ejection fraction: a comparative Doppler-conductance catheterization study,” Circulation, vol. 116, no. 6, pp. 637–647, 2007.
- S. R. Ommen, R. A. Nishimura, C. P. Appleton et al., “Clinical utility of Doppler echocardiography and tissue Doppler imaging in the estimation of left ventricular filling pressures: a comparative simultaneous Doppler-catheterization study,” Circulation, vol. 102, no. 15, pp. 1788–1794, 2000.
- V. Mor-Avi, R. M. Lang, L. P. Badano et al., “Current and evolving echocardiographic techniques for the quantitative evaluation of cardiac mechanics: ASE/EAE consensus statement on methodology and indications endorsed by the Japanese society of echocardiography,” European Journal of Echocardiography, vol. 12, no. 3, pp. 167–205, 2011.
- M. Cameli, M. Lisi, F. M. Righini et al., “Right ventricular longitudinal strain correlates well with right ventricular stroke work index in patients with advanced heart failure referred for heart transplantation,” Journal of Cardiac Failure, vol. 18, no. 3, pp. 208–215, 2012.
- A. Meris, F. Faletra, C. Conca et al., “Timing and magnitude of regional right ventricular function: a speckle tracking-derived strain study of normal subjects and patients with right ventricular dysfunction,” Journal of the American Society of Echocardiography, vol. 23, no. 8, pp. 823–831, 2010.
- M. Cameli, M. Caputo, S. Mondillo et al., “Feasibility and reference values of left atrial longitudinal strain imaging by two-dimensional speckle tracking,” Cardiovascular Ultrasound, vol. 7, no. 1, article 6, 2009.
- H.-K. Kim, D.-W. Sohn, S.-E. Lee et al., “Assessment of left ventricular rotation and torsion with two-dimensional speckle tracking echocardiography,” Journal of the American Society of Echocardiography, vol. 20, no. 1, pp. 45–53, 2007.
- U. Gustafsson, P. Lindqvist, S. Mörner, and A. Waldenström, “Assessment of regional rotation patterns improves the understanding of the systolic and diastolic left ventricular function: an echocardiographic speckle-tracking study in healthy individuals,” European Journal of Echocardiography, vol. 10, no. 1, pp. 56–61, 2009.
- F. Jeejeebhoy, M. Keith, M. Freeman et al., “Nutritional supplementation with MyoVive repletes essential cardiac myocyte nutrients and reduces left ventricular size in patients with left ventricular dysfunction,” American Heart Journal, vol. 143, no. 6, pp. 1092–1100, 2002.
- J. Azuma, A. Sawamura, and N. Awata, “Usefulness of taurine in chronic congestive heart failure and its prospective application,” Japanese Circulation Journal, vol. 56, no. 1, pp. 95–99, 1992.
- E. Giacomin, E. Palmerini, P. Ballo, V. Zacà, G. Bova, and S. Mondillo, “Acute effects of caffeine and cigarette smoking on ventricular long-axis function in healthy subjects,” Cardiovascular Ultrasound, vol. 6, article 9, 2008.
- A. F. Leite-Moreira, J. Correia-Pinto, and T. C. Gillebert, “Load dependence of left ventricular contraction and relaxation: effects of caffeine,” Basic Research in Cardiology, vol. 94, no. 4, pp. 284–293, 1999.
- S.-J. Dong, P. S. Hees, W.-M. Huang, S. A. Buffer Jr., J. L. Weiss, and E. P. Shapiro, “Independent effects of preload, afterload, and contractility on left ventricular torsion,” The American Journal of Physiology, vol. 277, no. 3, part 2, pp. H1053–H1060, 1999.
- M. B. Buchalter, F. E. Rademakers, J. L. Weiss, W. J. Rogers, M. L. Weisfeldt, and E. P. Shapiro, “Rotational deformation of the canine left ventricle measured by magnetic resonance tagging: effects of catecholamines, ischaemia, and pacing,” Cardiovascular Research, vol. 28, no. 5, pp. 629–635, 1994.
- D. E. Hansen, G. T. Daughters II, E. L. Alderman, N. B. Ingels, E. B. Stinson, and D. C. Miller, “Effect of volume loading, pressure loading, and inotropic stimulation on left ventricular torsion in humans,” Circulation, vol. 83, no. 4, pp. 1315–1326, 1991.
- E. Akagawa, K. Murata, N. Tanaka et al., “Augmentation of left ventricular apical endocardial rotation with inotropic stimulation contributes to increased left ventricular torsion and radial strain in normal subjects: quantitative assessment utilizing a novel automated tissue tracking technique,” Circulation Journal, vol. 71, no. 5, pp. 661–668, 2007.
- G. W. Yip, Y. Zhang, P. Y. Tan et al., “Left ventricular long-axis changes in early diastole and systole impact of systolic function on diastole,” Clinical Science, vol. 102, no. 5, pp. 515–522, 2002.
- Y. T. Tan, F. Wenzelburger, E. Lee et al., “The pathophysiology of heart failure with normal ejection fraction: exercise echocardiography reveals complex abnormalities of both systolic and diastolic ventricular function involving torsion, untwist, and longitudinal motion,” Journal of the American College of Cardiology, vol. 54, no. 1, pp. 36–46, 2009.
- R. A. Greenbaum, S. Y. Ho, and D. G. Gibson, “Left ventricular fibre architecture in man,” British Heart Journal, vol. 45, no. 3, pp. 248–263, 1981.
- N. B. Ingels Jr., D. E. Hansen, G. T. Daughters II, E. B. Stinson, E. L. Alderman, and D. C. Miller, “Relation between longitudinal, circumferential, and oblique shortening and torsional deformation in the left ventricle of the transplanted human heart,” Circulation Research, vol. 64, no. 5, pp. 915–927, 1989.
- S.-J. Park, C. Miyazaki, C. J. Bruce, S. Ommen, F. A. Miller, and J. K. Oh, “Left ventricular torsion by two-dimensional speckle tracking echocardiography in patients with diastolic dysfunction and normal ejection fraction,” Journal of the American Society of Echocardiography, vol. 21, no. 10, pp. 1129–1137, 2008.