Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum: Live Cell Labeling with Monoclonal Antibodies
Figure 3
The effects of low levels of sugars upon cell growth. (a) represents a control cell 48 h after labeling with JIM5. New HG secretion/wall growth is the dark or unlabeled zone in the cell center. Scale bar = 10.2 μm. (b) is the cell in (a) labeled with SYTO9 and highlights the nucleus (arrow). Scale bar = 10 μm. (c) represents a cell treated in 4% mannitol for 48 h. This cell is greatly expanded, Scale bar = 16 μm. (d) is the cell in (c) labeled with SYTO9 and highlights the two nuclei (arrows). Scale bar = 16 μm. (e) is a cell after 48 h in 4% mannitol. Scale bar = 20 μm. (f) is a cell after 48% in 4% glucose. Scale bar = 17 μm. (g) is a cell after 48 h in 4% sucrose. Scale bar = 18 μm. Figures 4(c), 4(e), 4(f), and 4(g) are labeled with JIM5, and all images were acquired using FLM.
