Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.) Woodson by Ultraviolet Spectroscopy

Table 1

Data of repeatability, intermediate precision, and accuracy of the developed UV spectrophotometric method for determination of total alkaloids in aqueous extract of H. lancifolius.

Assay	Calculated concentration (𝜇g/mL)	𝑁	𝐶 (𝜇g/mL)	SD	RSD (%)	𝐴 (%)
Repeatability	16	3	15.698	0.030	0.19	98.00
	20	3	19.864	0.181	0.90	99.32
	28	3	27.724	0.392	1.41	99.01
Intermediary precision; day 1	16	3	16.448	0.205	1.25	102.80
	20	3	20.580	0.257	1.25	102.90
	28	3	28.373	0.245	0.86	101.33
Intermediary precision; day 2	16	3	16.213	0.339	2.09	101.33
	20	3	20.345	0.054	0.27	101.72
	28	3	28.391	0.271	0.96	101.39

𝐶 = averaged concentration from 𝑁 determinations; SD = standard deviation; RSD (%) = relative standard deviation; 𝐴 (%) = accuracy.