Advanced Glycation End Products Increase MDM2 Expression via Transcription Factor KLF5

Figure 3

AGEs trigger signaling via their receptor RAGE. Cells were pretreated with RAGE siRNA or anti-RAGE antibody followed by treatment with BSA-AGEs (50 μg/ml) for 24 hours. Both siRNA and anti-RAGE inhibited the effects of AGEs on the levels of MDM2, Rb, and p53. (b–e) The bands of p53 (b), Rb (c), p-Rb (d), and MDM2 (e) in Figure 3(a) were quantified using ImageJ. Every experiment was repeated at least three times. Asterisks denote significant difference amongst experimental groups. and .

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