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Journal of Energy
Volume 2014 (2014), Article ID 483813, 7 pages
http://dx.doi.org/10.1155/2014/483813
Research Article

Microwave Assisted Alkali Pretreatment of Rice Straw for Enhancing Enzymatic Digestibility

1Centre for Environment Science and Climate Resilient Agriculture, IARI, New Delhi 110012, India
2Civil Engineering, Architecture and Building (CAB), Faculty of Engineering and Computing, Coventry University, Priory Street, Coventry CV1 5FB, UK

Received 8 August 2013; Accepted 6 January 2014; Published 25 March 2014

Academic Editor: S. Venkata Mohan

Copyright © 2014 Renu Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rapid industrialization, increasing energy demand, and climate change are the conditions that forced the researchers to develop a clean, efficient, renewable, and sustainable source of energy which has a potential to replace fossil fuels. Ethanol is one of the attractive and suitable renewable energy resources. In present study, effectiveness of microwave pretreatment in combination with sodium hydroxide (NaOH) for increasing enzymatic hydrolysis of rice straw has been investigated and under optimum conditions obtained a maximum reducing sugar (1334.79 µg/mL) through microwave assisted NaOH pretreatment. Chemical composition analysis and scanning electron microscope (SEM) images showed that the removal of lignin, hemicellulose, and silicon content is more in microwave assisted NaOH pretreatment than the blank sample. X-ray diffraction (XRD) analysis revealed that the crystallinity index of rice straw treated with microwave assisted alkali (54.55%) is significantly high as compared to the blank (49.07%). Hence, the present study proves that microwave assisted alkali pretreatment can effectively enhance enzymatic digestibility of rice straw and it is feasible to convert rice straw for bioethanol production.

1. Introduction

In recent times, due to increase in concerns for climate change and greenhouse gas emissions, research work has been inclined towards the development of sustainable and renewable energy resources. The atmospheric CO2 levels increased from ~275 to ~380 ppm [1]. From renewable resources, ethanol has been of great interest as an alternative fuel or oxygenate additive for fossil fuels. In 2005 and 2006, worldwide production capacity of ethanol was about 45 and 49 billion litres, respectively, and the total projected demand in 2015 is over 115 billion litres [2]. Lignocellulosic materials are abundant, cheap, and renewable and may be used as a substrate for ethanol production through microbial intervention [3]. Cereal straws are the most abundant resource which can serve as a potential feedstock for the production of biofuel [4]. Rice contains 23% straw of its total weight. Usually, farmers burnt the rice straw left on the field in order to clear the field for the next crop. In India, open-field burning of rice straw contributes up to 0.05% of total GHG emissions [5]. Use of rice straw for bioethanol production not only provides a renewable fuel but also prevents climate change.

Rice straw is mainly composed of cellulose, hemicellulose, lignin, silica, and ash contents. Conversion of rice straw to fermentable sugar is a very complicated process due to the presence of complex structure of lignin and hemicelluloses with cellulose [68]. A pretreatment process is required as it can remove cellulose and hemicellulose, reduce cellulose crystallinity, and increase the porosity of the materials [9]. Vast varieties of pretreatment methods are available such as steam explosion, liquid hot water, dilute acid, flow through acid pretreatment, lime, wet oxidation and ammonia fiber/freeze explosion, milling and grinding, microwave energy, wet oxidation, and high energy radiation [10, 11].

Microwave irradiation has high heating efficiency, and thus it is able to degrade lignin and hemicelluloses as well as increase in enzymatic susceptibility [12]. Microwave heats the target object directly by applying an electromagnetic field to dielectric molecules as compared to conduction/convection heating which is based on intramolecular heat transfer [13]. It can significantly increase the enzymatic hydrolysis [1416] of rice straw. Several researchers have used microwave pretreatment as a potential method for pretreatment of various lignocellulosic materials [1722] and to damage the recalcitrant lignin [23]. Microwave-chemical pretreatment can be proved as a beneficial method for hydrolysis of rice straw; however, extensive research work on using microwave-chemical pretreatment method for rice straw has not been done effectively.

In present research work, microwave treatment is combined with different concentration of alkali, that is, sodium hydroxide (NaOH) for enzymatic hydrolysis. Response surface methodology (RSM) is used for statistical analysis. Response surface methodology (RSM) is a collection of mathematical and statistical techniques for empirical model building. By careful design of experiments, the objective is to optimize a response (output variable) which is influenced by several independent variables (input variables). For optimization, the user required to supply minimum and maximum values for each factor [24]. For Indian rice straw variety, alkali pretreatment is not yet explored along with microwave treatment. In this work, for systematic study of effectiveness of microwave assisted NaOH, a Box-Behnken design was selected for the optimization of pretreatment conditions. Further, the morphological characteristics of rice straw are analyzed through scanning electron microscope (SEM) and biomass crystallinity is analyzed through X-ray diffraction (XRD).The aim of this study is to optimize an efficient microwave pretreatment technology for the hydrolysis of rice straw for ethanol production.

2. Material and Methods

2.1. Raw Materials and Microwave Alkali Pretreatment

In the present research work Indian straw from rice variety “Pusa Sugandh” has been used. The samples of rice straw were locally harvested at Indian Agriculture Research Institute. Firstly, rice straw has been cut into pieces of size 1-2 cm. Now the prepared samples of rice straw are cleaned thoroughly using tap water until the washings became clean and colorless. Before any pretreatment, samples have been air dried. The chemical composition of rice straw is given in Table 1. Microwave pretreatment is one of the efficient ways and modified type domestic microwave oven is used in the present study. The microwave power varied between 70 and 700 W, respectively. About 5 g of rice straw was suspended in 30 mL of NaOH concentration ranged from 0.1 to 2% and left for overnight as per RSM fitted design. It was then radiated in the range of 70–700 W for 1–5 min in microwave. All the pretreatment conditions, that is, power 70–700 W, concentration of chemicals 0.1 to 2%, and treatment time 1 to 5 min, are designed by Response Surface Model (RSM), Design Expert software Version 7.

tab1
Table 1: Chemical composition of rice straw variety (Pusa Sugandh) [45].
2.2. Enzymatic Saccharification of Pretreated Rice Straw

Saccharification or hydrolysis of the wet pretreated paddy straw samples is carried out using E-CELAN, endo-1, 4-β-glucanase from Aspergillus niger supplemented with EBGLUC (endo-β-glucosidase), and β-glucosidase from Aspergillus niger (Megazyme International and Genencor) [25]. All other chemicals employed in this study are of reagent grade. Enzyme saccharification is carried out in 50 mL screw capped bottles, which consisted of 1.0 g microwave treated rice straw, 10 units of E-CELAN, and 5 units of EBGLUC. The final volume of reaction mixture has been made using 10 mL of citrate buffer (pH 4.8). Bottles are kept at 50°C and 150 rpm in a constant temperature shaker water bath. Samples have been collected from reaction mixture at different time intervals and analyzed for sugar by DNSA method [26]. All the experiments have been performed in triplicate and the average values are reported.

2.3. Morphological Characterization through Scanning Electron Microscope (SEM)

In this study, the morphology of rice straw is examined through scanning electron microscope (ZEISS, Evoma-10). Firstly, samples are dried in a vacuum dryer oven at 45°C for 24 h and then gradually dehydrated using acetone-water mixtures. Same process is being repeated with 50%–100% acetone. The samples have been mounted on aluminium stubs and coated with gold and platinum mixtures prior to imaging under SEM.

2.4. X-Ray Diffraction (XRD) of the Pretreated Raw Materials

Crystallinity of untreated and pretreated rice straw samples has been determined using X-ray diffraction (PW 1710, copper Kα radiation). Rice straw treated with water-microwave served as a control. Crystallinity index is calculated by using the following formulae [27]: where is intensity for the crystalline part of the biomass (i.e., cellulose) and is intensity for the amorphous part of the biomass (i.e., cellulose, hemicellulose, and lignin). In this research work, intensity of crystalline portion was at and intensity for amorphous portion was at .

For the estimation of comprising crystalline area in plant () (2) is used to calculate crystalline size of (002) plane based on Scherrer equation [28]: where is wavelength of X-ray tube ( Å), is FWHM (full width at half maximum) of (002) peak, and is diffraction angle of (002) plane.

2.5. Removal and Recovery of Lignin

The extent of lignin removal is mainly determined on the basis of lignin fragments and monomers present in the alkali extract according to the NREL LAP-004. The absorbance is measured at 205 nm through spectrophotometer [29]. Through acidification, value added acid-precipitable polymeric lignins are recuperated from the extracts [30]. In the next step extract is acidified to pH 1-2 with concentrated sulphuric acid. Centrifugation process took 30 minutes at 13000 rpm. The precipitates are washed with distilled water and dried at 60°C till the constant weight has been achieved.

2.6. Experimental Designs and Data Analysis

Design Expert software Version 7 named Box-Behnken factorial design (BBD) is used with three factors and three levels, including three replicated at centre point to evaluate the effect of concentration of chemicals (), power (), and treatment time () on hydrolysis of rice straw () obtained from the pretreatment experiments. The range of variables for NaOH is power 70–700 W, concentration of chemicals 0.1% to 2%, and treatment time 1 to 5 min. The design matrix with 17 experimental designs runs in one block with five replicates. A polynomial quadratic equation was fitted to evaluate the effect of each independent variable to the response: where is the predicted response; is a constant; , , and are the linear coefficients; , , and are the cross-coefficients; , , and are the quadratic coefficients. The response surfaces of the variables inside the experimental domain were analyzed using Design Expert. Subsequently, five additional confirmation experiments were conducted to verify the validity of the statistical experimental strategies.

3. Results and Discussion

3.1. Response Surface Methodology (RSM) Results

For optimization of microwave effect and other factors on saccharification of rice straw, experiments based on BBD model are employed. Design expert software is used for data analysis, analysis of variance (ANOVA), regression coefficients, and regression equations. ANOVA model represents that model is significant for NaOH at Fisher’s -test value 13.23 (Table 2). The coefficient of variation () for NaOH has been found as 0.94. The model appears statistically sound as the lack of fit test used for testing of model shows value of 0.0784 and it is not significant. The most significant parameter for NaOH is treatment time () and quadratic terms NaOH concentration (), power (), and time (). Analysis of residuals showed no abnormality. The 3D response surfaces for NaOH treatment are shown in Figure 1. To depict the interactive effect of independent variables on responses, one variable should be kept constant while the other two variables varied at different ranges. The interaction between different factors has been shown through the shape of response surfaces.

tab2
Table 2: ANOVA of the quadratic model microwave and NaOH pretreatment and its influential factors.
483813.fig.001
Figure 1: Response surface for the effect on reducing sugar using power (microwave) and NaOH concentration at constant time.
3.2. Optimum Conditions

Same Design Expert software is used for deciding optimum conditions (Table 3). The reducing sugar obtained through NaOH-microwave pretreatment under optimum condition is 1334.79 μg/mL (Table 4). The reducing sugar concentration in the saccharified rice straw under NaOH-microwave pretreatment was increased 1752% (Table 3) as compared to raw straw.

tab3
Table 3: Optimum conditions for delignification of rice straw.
tab4
Table 4: Predicted and experimental reducing sugar obtained under optimum conditions.
3.3. Scanning Electron Microscope (SEM) Analysis

The morphological changes that occurred due to pretreatment could be analyzed with the help of scanning electron microscope (SEM) [31]. SEM images of the untreated sample showed that there is less number of cracks and the surfaces of the samples are densely packed as compared to NaOH-microwave pretreated (Figure 2). The silicon waxy structure is ruptured and lignin-hemicellulosic complex of NaOH-microwave pretreated rice straw is broken down drastically. Previous studies have also shown that the surface of the samples treated with microwave assisted organic acid became loose and irregular [6]. Also, microwave assisted FeCl3 damaged the cell wall structure and altered the fibrillar structure of rice straw [32]. It proves that microwave pretreatment has effectively improved the straw digestibility by removing silica content [33].

fig2
Figure 2: SEM images of (a) untreated sample and (b) sample pretreated with microwave assisted sodium hydroxide (NaOH).
3.4. Effect on Chemical Composition of Rice Straw

Chemical components of rice straw changed after pretreatment with microwave assisted treatment containing NaOH (Table 5). There is increase in percentage of cellulose contents in treated rice straw samples with comparison to untreated. However other components, for example, lignin, hemicellulose, and ash, have been reduced significantly. This indicates that the pretreatment method is capable of removing lignin and other components. It damaged the cell wall by disrupting the lignin structure. It led to increase in the surface area and thereby better enzymatic accessibility. All these conditions are greatly beneficial for enzymatic hydrolysis.

tab5
Table 5: Chemical composition of rice straw after pretreatment.
3.5. X-Ray Diffraction (XRD) Analysis

Crystallinity index is the percentage of crystalline material in the biomass [28]. It is a major factor that affected enzymatic hydrolysis [34, 35]. XRD analysis (Figure 3) shows that the crystallinity index of rice straw treated with microwave assisted NaOH is high as compared to the untreated and blank sample. For untreated and blank (without addition of any chemicals) sample, it is 52.2% and 49.07%, respectively, as listed in Table 6. By disrupting inter- and intrachain hydrogen bonding of cellulose fibrils pretreatments can change the cellulose structure [36]. In biomass, hemicellulose and lignin are amorphous in nature while cellulose is crystalline [37]. The results demonstrated that removal of amorphous parts of the rice straw, that is, lignin and hemicellulose, was more in sample treated with microwave assisted NaOH than untreated and blank. So, microwave-assisted NaOH pretreated rice straw had lower lignin and hemicellulose, but higher cellulose. It is being found that cellulose content has been increased but only in small amount, whereas imperfect microcrystalline cellulose has been hydrolyzed and large perfect cellulose was left. Previous research has also suggested that the crystallinity index of rice straw could enhance by hot acid treatment [38]. Several studies showed increase in crystalline index value after biomass pretreatment [3943].

tab6
Table 6: Crystallinity index of rice straw samples.
fig3
Figure 3: X-ray diffraction pattern of (a) untreated sample, (b) blank, and (c) NaOH-microwave treated sample.

Scanning electron microscope (SEM) analysis, changes on chemical composition of rice straw, and X-ray diffraction (XRD) analysis, used in the present study, proved that microwave assisted sodium hydroxide pretreatment method had the potential of exposing cellulose and increasing cellulose contents. Similar results were obtained from previous research studies. They also found it beneficial as the microwave irradiation could enhance the lignin degradation and presence of aqueous NaOH increases saponification of intermolecular ester bonds cross-linking hemicellulose and lignin [44].

The study also proves that huge availability of rice straw in Indian livestock has tremendous potential for ethanol conversion using microwave-chemical pretreatment methods and technology is working well for them in Indian conditions and varieties of rice straw.

4. Conclusions

The present research work proves that microwave is an efficient heating method for the pretreatment of rice straw. The combination of microwave pretreatment with NaOH increases the saccharification of rice straw by removing lignin and hemicelluloses in large quantity and increases its accessibility to enzymes, respectively. The optimal conditions have been deducted by using Box-Behnken design (BBD). Maximum reducing sugar was obtained through microwave assisted NaOH pretreatment (1334.79 μg/mL) under optimum conditions as compared to the blank. Analysis of chemical composition of rice straw, the images obtained through scanning electron microscope (SEM), and X-ray diffraction (XRD) analysis showed the removal of lignin and hemicellulose, although lignin has not been recovered significantly. SEM images showed that the surface rupture is more in microwave assisted NaOH pretreatment than blank sample. Crystallinity index for rice straw samples treated with microwave assisted NaOH is significantly high 54.55% in comparison to untreated sample 52.2%. The removal of lignin and hemicellulose increased the enzyme accessibility with microwave treatment and thus the enzymatic saccharification of rice straw can be assisted with microwave efficiently.

Conflict of Interests

The authors declare that they have no conflict of interests.

Acknowledgment

The authors are grateful to Science and Engineering Research Board, Department of Science and Technology, Government of India, for providing funding during the course of the study.

References

  1. A. J. Ragauskas, C. K. Williams, B. H. Davison et al., “The path forward for biofuels and biomaterials,” Science, vol. 311, no. 5760, pp. 484–489, 2006. View at Publisher · View at Google Scholar · View at Scopus
  2. S. A. Ravoof, K. Prateepa, T. Supassri, and S. Chittibabu, “Enhancing enzymatic hydrolysis of rice straw using microwave-assisted nitric acid pretreatment,” International Journal of Medicine and Biosciences, vol. 1, no. 3, pp. 13–17, 2012.
  3. X. B. Zhao, L. Wang, and D. Liu, “Effect of several factors on peracetic acid pretreatment of sugarcane bagasse for enzymatic hydrolysis,” Journal of Chemical Technology and Biotechnology, vol. 82, no. 12, pp. 1115–1121, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. R. C. Sun, Cereal Straw as a Resource for sustainable biomaterials and Biofuels, Elsevier, Amsterdam, The Netherlands, 2010.
  5. B. Gadde, C. Menke, and R. Wassmann, “Rice straw as a renewable energy source in India, Thailand, and the Philippines: overall potential and limitations for energy contribution and greenhouse gas mitigation,” Biomass and Bioenergy, vol. 33, no. 11, pp. 1532–1546, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. G. Gong, D. Liu, and Y. Huang, “Microwave-assisted organic acid pretreatment for enzymatic hydrolysis of rice straw,” Biosystems Engineering, vol. 107, no. 2, pp. 67–73, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. Y. Sun and J. Cheng, “Hydrolysis of lignocellulosic materials for ethanol production: a review,” Bioresource Technology, vol. 83, no. 1, pp. 1–11, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. S. D. Zhu, Pretreatment by microwave/alkali of rice straw and its saccharification and fermentation ethanol production [Ph.D. thesis], Huazhong Agriculture University, Wuhan, China, 2005.
  9. K. Karimi, G. Emtiazi, and M. J. Taherzadeh, “Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae,” Enzyme and Microbial Technology, vol. 40, no. 1, pp. 138–144, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. C. G. Liu and C. E. Wyman, “Partial flow of compressed-hot water through corn stover to enhance hemicellulose sugar recovery and enzymatic digestibility of cellulose,” Bioresource Technology, vol. 96, no. 18, pp. 1978–1985, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. L. T. Fan, Y. H. Lee, and M. M. Gharpuray, “The nature of ligno-cellulosics and their pretreatments for enzymatic hydrolysis,” Advances in Biochemical Engineering, vol. 23, pp. 157–187, 1982.
  12. S. Zhu, Y. Wu, Z. Yu, J. Liao, and Y. Zhang, “Pretreatment by microwave/alkali of rice straw and its enzymic hydrolysis,” Process Biochemistry, vol. 40, no. 9, pp. 3082–3086, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. R. E. Newnham, S. J. Jang, M. Xu, and F. Jones, “Fundamental interaction mechanisms between microwaves and matter,” in Ceramic Tranctions, Microwaves: Theory and Application in Materials Processing, D. E. Clark, F. D. Gac, and W. H. Sutton, Eds., vol. 21, America Ceramic Society, Westerville, Ohio, USA, 1991.
  14. J. Azuma, F. Tanaka, and T. Koshijima, “Enhancement of enzymatic susceptibility of ligno-cellulosic wastes by microwave irradiation,” Journal of Fermentation Technology, vol. 63, pp. 377–384, 1984.
  15. H. Ooshima, K. Aso, Y. Harano, and T. Yamamoto, “Microwave treatment of cellulosic materials for their enzymatic hydrolysis,” Biotechnology Letters, vol. 6, no. 5, pp. 289–294, 1984. View at Publisher · View at Google Scholar · View at Scopus
  16. P. Kitchaiya, P. Intanakul, and M. Krairiksh, “Enhancement of enzymatic hydrolysis of lignocellulosic wastes by microwave pretreatment under atmospheric pressure,” Journal of Wood Chemistry and Technology, vol. 23, no. 2, pp. 217–225, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. C. Eskicioglu, K. J. Kennedy, and R. L. Droste, “Enhancement of batch waste activated sludge digestion by microwave pretreatment,” Water Environment Research, vol. 79, no. 11, pp. 2304–2317, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Eskicioglu, N. Terzian, K. J. Kennedy, R. L. Droste, and M. Hamoda, “Athermal microwave effects for enhancing digestibility of waste activated sludge,” Water Research, vol. 41, no. 11, pp. 2457–2466, 2007. View at Publisher · View at Google Scholar · View at Scopus
  19. M. J. Taherzadeh and K. Karimi, “Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review,” International Journal of Molecular Sciences, vol. 9, no. 9, pp. 1621–1651, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. P. Alvira, E. Tomás-Pejó, M. Ballesteros, and M. J. Negro, “Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review,” Bioresource Technology, vol. 101, no. 13, pp. 4851–4861, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. J. Shi, Y. Pu, B. Yang, A. Ragauskas, and C. E. Wyman, “Comparison of microwaves to fluidized sand baths for heating tubular reactors for hydrothermal and dilute acid batch pretreatment of corn stover,” Bioresource Technology, vol. 102, no. 10, pp. 5952–5961, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. D. Jackowiak, J. C. Frigon, T. Ribeiro, A. Pauss, and S. Guiot, “Enhancing solubilisation and methane production kinetic of switchgrass by microwave pretreatment,” Bioresource Technology, vol. 102, no. 3, pp. 3535–3540, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. Z. Hu and Z. Wen, “Enhancing enzymatic digestibility of switchgrass by microwave-assisted alkali pretreatment,” Biochemical Engineering Journal, vol. 38, no. 3, pp. 369–378, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. S. L. C. Ferreira, R. E. Bruns, E. G. P. da Silva et al., “Statistical designs and response surface techniques for the optimization of chromatographic systems,” Journal of Chromatography A, vol. 1158, no. 1-2, pp. 2–14, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. B. L. Manjunath, H. R. Prabhu Desai, S. Talaulikar, and V. S. Korikanthimath, “Selection of scented rice (Oryza sativa) and its value-addition for higher profitability,” Indian Journal of Agricultural Sciences, vol. 78, no. 8, pp. 663–666, 2008. View at Scopus
  26. M. Saritha, A. Arora, and L. Nain, “Pretreatment of paddy straw with Trametes hirsuta for improved enzymatic saccharification,” Bioresource Technology, vol. 104, pp. 459–465, 2012. View at Publisher · View at Google Scholar · View at Scopus
  27. G. L. Miller, “Use of Dinitro-salicyclic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, pp. 1843–1848, 1959.
  28. L. Segal, J. Creely, A. Martin, and C. Conrad, “An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer,” Textile Research Journal, vol. 29, pp. 786–794, 1959.
  29. E. Gümüşkaya and M. Usta, “Crystalline structure properties of bleached and unbleached wheat straw (Triticum aestivum L.) soda-oxygen pulp,” Turkish Journal of Agriculture and Forestry, vol. 26, no. 5, pp. 247–252, 2002. View at Scopus
  30. T. Ehrman, “Determination of Acid-soluble Lignin in biomass,” NREL Chemical Analysis and Testing Task Laboratory Analytical Procedure-004, pp. 1–7, 1996.
  31. A. L. Pometto III and D. L. Crawford, “Catabolic fate of Streptomyces viridosporus T7A-produced, acid-precipitable polymeric lignin upon incubation with ligninolytic Streptomyces species and Phanerochaete chrysosporium,” Applied and Environmental Microbiology, vol. 51, no. 1, pp. 171–179, 1986. View at Scopus
  32. C. Namasivayam and D. Kavitha, “IR, XRD and SEM studies on the mechanism of adsorption of dyes and phenols by coir pith carbon from aqueous phase,” Microchemical Journal, vol. 82, no. 1, pp. 43–48, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. G. Gong, D. Liu, and Y. Huang, “Microwave-assisted organic acid pretreatment for enzymatic hydrolysis of rice straw,” Biosystems Engineering, vol. 107, no. 2, pp. 67–73, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. J. Lu and P. Zhou, “Optimization of microwave-assisted FeCl3 pretreatment conditions of rice straw and utilization of Trichoderma viride and Bacillus pumilus for production of reducing sugars,” Bioresource Technology, vol. 102, no. 13, pp. 6966–6971, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. T. Řezanka and K. Sigler, “Biologically active compounds of semi-metals,” Phytochemistry, vol. 69, no. 3, pp. 585–606, 2008. View at Publisher · View at Google Scholar · View at Scopus
  36. T. H. Kim and Y. Y. Lee, “Pretreatment and fractionation of corn stover by ammonia recycle percolation process,” Bioresource Technology, vol. 96, no. 18, pp. 2007–2013, 2005. View at Publisher · View at Google Scholar · View at Scopus
  37. J. P. O'Dwyer, L. Zhu, C. B. Granda, and M. T. Holtzapple, “Enzymatic hydrolysis of lime-pretreated corn stover and investigation of the HCH-1 model: inhibition pattern, degree of inhibition, validity of simplified HCH-1 Model,” Bioresource Technology, vol. 98, no. 16, pp. 2969–2977, 2007. View at Publisher · View at Google Scholar · View at Scopus
  38. N. Mosier, C. Wyman, B. Dale et al., “Features of promising technologies for pretreatment of lignocellulosic biomass,” Bioresource Technology, vol. 96, no. 6, pp. 673–686, 2005. View at Publisher · View at Google Scholar · View at Scopus
  39. T. Jeoh, C. I. Ishizawa, M. F. Davis, M. E. Himmel, W. S. Adney, and D. K. Johnson, “Cellulase digestibility of pretreated biomass is limited by cellulose accessibility,” Biotechnology and Bioengineering, vol. 98, no. 1, pp. 112–122, 2007. View at Publisher · View at Google Scholar · View at Scopus
  40. C. T. Yu, W. H. Chen, L. C. Men, and W. S. Hwang, “Microscopic structure features changes of rice straw treated by boiled acid solution,” Industrial Crops and Products, vol. 29, no. 2-3, pp. 308–315, 2009. View at Publisher · View at Google Scholar · View at Scopus
  41. V. S. Chang and M. T. Holtzapple, “Fundamental factors affecting biomass enzymatic reactivity,” Applied Biochemistry and Biotechnology, vol. 84–86, pp. 5–37, 2000. View at Scopus
  42. S. Kim and M. T. Holtzapple, “Effect of structural features on enzyme digestibility of corn stover,” Bioresource Technology, vol. 97, no. 4, pp. 583–591, 2006. View at Publisher · View at Google Scholar · View at Scopus
  43. J. S. Bak, J. K. Ko, Y. H. Han, B. C. Lee, I. G. Choi, and K. H. Kim, “Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment,” Bioresource Technology, vol. 100, no. 3, pp. 1285–1290, 2009. View at Publisher · View at Google Scholar · View at Scopus
  44. L. Liu, J. Sun, M. Li, S. Wang, H. Pei, and J. Zhang, “Enhanced enzymatic hydrolysis and structural features of corn stover by FeCl3 pretreatment,” Bioresource Technology, vol. 100, no. 23, pp. 5853–5858, 2009. View at Publisher · View at Google Scholar · View at Scopus
  45. H. Ma, W. W. Liu, X. Chen, Y. J. Wu, and Z. L. Yu, “Enhanced enzymatic saccharification of rice straw by microwave pretreatment,” Bioresource Technology, vol. 100, no. 3, pp. 1279–1284, 2009. View at Publisher · View at Google Scholar · View at Scopus