Comparative Study of Regulatory T Cell Function of Human T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood
Figure 5
Weak suppressive activity and
expression of FOXP3 and CD152 proteins by
CD45RA+ type APB CD25+CD4+ T cells.
(a) CD45RA+CD25+CD4+,
CD45RA−CD25+CD4+, and CD25−CD4+ cells
were sorted and incubated under the indicated conditions as described in Section
2. Cell proliferation was assessed by 3H-thymidine
uptake on day 4. Data are representative
of 3 different donors. (b) Mononuclear
cells from APB were multistained with anti-CD4, anti-CD8, anti-CD25,
anti-CD45RA, anti-FOXP3, and anti-CD152 Abs as described in Table 1. The profiles were gated initially for CD8−CD4+,
then CD45RA− type or CD45RA+ type CD25+CD4+ T cells were gated based on the
expression of CD45RA and CD25 in panel (a). The expressions of CD25 versus endogenous FOXP3 or CD152 in CD45RA− type (panels (b) and (c)) or CD45RA+ type CD25+CD4+ T cells (panels (d) and (e)) were merged as blue dots to those in CD8−CD4+ cells (red dots in panels (b)–(e)). Data are
representative of 10 different donors. (c) Mononuclear cells or thymocytes were multistained as described in
Figure 4(b). On the basis of FOXP3
expression, FOXP
or FOXP was gated (panels
(a), (d), (f)). In each population, histogram of
CD45RA expression was shown (panels (b), (c), (e), (g), (h)).
