Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Figure 2
Cytokine expression by stimulated splenocytes from F1-V vaccinated wild-type C3H/HeN, Tlr2, Tlr4, and Tlr2/4 deficient mice appears to be dependent on Tlr4. Mice were given a prime-boost vaccination of F1-V (1 μg) as shown in Figure 1, and splenocytes from these same mice were prepared and stimulated for 44 h in triplicate with medium only, 4 μg of F1-V or V antigen. Culture supernatants were collected and cytokine expression was determined: (a) IFN-; (b) IL-4; (c) IL-10. The results are reported as mean with standard error of the mean. Statistical significance shown above the respective bar was reported for differences in cytokine expression between splenocytes from wild-type C3H/HeN and Tlr4 and Tlr2/4-deficient C3H/HeN mice stimulated with F1-V.
