Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice

Figure 3

Proliferation by stimulated splenocytes from F1-V vaccinated wild-type C3H/HeN, Tlr2, Tlr4, and Tlr2/4-deficient mice appears to be dependent on Tlr4. Proliferation was determined after 44 h incubation in the presence of 4 μg of F1-V or V-antigen and a further 24 h incubation in the presence of 3[H]-Thymidine. Splenocytes used in the assay were prepared from the same groups of mice as shown in Figure 1. The results are reported as mean with standard error of the mean. The results on the 𝑦-axis are plotted in linear because of the overall low amount of stimulation. Statistical significance shown above the respective bar was reported for differences between the amount of proliferation between splenocytes from wild-type C3H/HeN and Tlr2 and Tlr2/4-deficient C3H/HeN mice stimulated with F1-V.