PGE₂-Induced IDO1 Inhibits the Capacity of Fully Mature DCs to Elicit an In Vitro Antileukemic Immune Response

Figure 4

IDO1 expression inhibited leukemia-specific IFN-γ production. AML cells were lysed and used for the pulsing of immature Mo-DCs. Mo-DCs were subsequently matured with the cytokine cocktail containing PGE₂. Autologous CD3⁺ T cells were cocultured with the preloaded and matured DCs for an overnight, in the presence or absence of the IDO1-specific inhibitor 1-MT-L. As control samples, unloaded Mo-DCs were used (data not shown). (a) CD3⁺ T cells, (b) CD4⁺ T cells, and (c) CD8⁺ T cells were tested for leukemia-specific intracellular IFN-γ production. Results are expressed as the mean ± SEM of 3 independent experiments. , versus w/o DCs or versus medium alone.

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(b)

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