Dynamic Probing of Nanoparticle Stability In Vivo: A Liposomal Model Assessed Using In Situ Microdialysis and Optical Imaging

Scheme 1

The microdialysis probe with total diameter of 360 𝜇m and molecular size cutoff at 100 KDa was in situ implanted for simultaneous measurements of fluorescence intensity of extracellular fluid. If the liposomes are ruptured, the encapsulated CF molecules will be leaching out and collected by microdialysis probe. Thus, the stability of liposomes on site in liver can be dynamically assessed by the change of fluorescence intensity in microdialysates.