Unique Mode of Lipogenic Activation in Rat Preputial Sebocytes

Figure 4

Comparison of the expression of lipogenic regulatory genes in freshly dispersed (Fr) and cultured (Cx) ± dihydrotestosterone (DHT) preputial sebocytes and epidermal cells, using RT-PCR. Total RNA was isolated from adult rat preputial sebocytes, epidermal cells and homogenized epididymal fat pad. Primers specific for the listed genes were used (see Table 1). Adipsin, aP2, CD36, and MC5-R were only detected in sebocytes, not in fresh or cultured epidermal cells. Leptin was detected in cultured sebocytes (less mature) to a greater extent than in freshly dispersed sebocytes (more mature) or epidermal cells. MC5-R was detected in freshly dispersed sebocytes to a greater extent than cultured sebocytes.