Studies on Multifunctional Effect of All-Trans Retinoic Acid (ATRA) on Matrix Metalloproteinase-2 (MMP-2) and Its Regulatory Molecules in Human Breast Cancer Cells (MCF-7)
Figure 6
(a) Western blot analysis of FAK: MCF-7 (300,000 cells/1.5 mL) cells were grown in absence (lane C) and presence of 30 M ATRA (lane E) for 24 hours in SFCM. The cells were collected and were extracted in cell extraction buffer. 50 g of protein from both control and ATRA-treated cell extracts were run on 7.5% SDS-PAGE and the proteins were transferred onto nitrocellulose membrane by western blot. The membrane was incubated with anti-FAK antibody followed by incubation with alkaline phosphatase coupled secondary antibody. Bands were visualized using NBT-BCIP as substrate. Quantitative measurements of immunoblot was done by using Image J Launcher (version 1.4.3.67). (C) represents the expression of respective proteins in control cells whereas (E) represents expression in 30 M ATRA treated cells. (b) RT-PCR of FAK: Figure 6(b) showed the status of FAK mRNA expression in control (lane C) and ATRA treated (lane E) MCF-7 cells. A quantitative measurement of RT-PCR (Figure 6(b)) was done by using Image J Launcher (version 1.4.3.67). (C) represents the expression of FAK in control cells whereas (E) represents FAK expression 30 M ATRA treated cells in respective figures.
