Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse

Table 4

Point-by-point comparisons of FTIRI Data (illustrated in Figure 5).

(a) Mineral/Matrix

Week	Tissue	CO	EC	IM	WB
1	CC	<0.001	0.197	0.099	<0.001
	CO		<0.001	<0.001	<0.001
	EC			0.999	0.484
	IM				0.282
2	CC	0.009	0.966	0.999	0.998
	CO		<0.001	<0.001	0.001
	EC			0.421	0.121
	IM				0.999
4	CC	—	—	—	—
	CO		—	<0.001	0.005
	EC			—	—
	IM				0.542
8	CC	—	—	—	—
	CO		—	0.020	<0.001
	EC			—	—
	IM				0.971
Tissue	Week	2	4	8
CC	1	<0.001	—	—
	2		—	—
	4			—
CO	1	0.887	0.903	0.999
	2		0.999	0.793
	4			0.783
EC	1	0.009	—	—
	2		—	—
	4			—
IM	1	<0.001	<0.001	<0.001
	2		0.160	0.340
	4			0.999
WB	1	0.001	<0.001	<0.001
	2		0.628	0.044
	4			0.946

(b) Carbonate/phosphate

Week	Tissue	CO	EC	IM	WB
1	CC	<0.001	0.579	0.982	<0.001
	CO		<0.001	0.035	0.082
	EC			0.933	0.357
	IM				0.324
2	CC	<0.001	<0.001	<0.001	<0.001
	CO		0.028	0.997	0.009
	EC			0.048	0.548
	IM				0.003
4	CC	—	—	—	—
	CO		—	0.826	0.999
	EC			—	—
	IM				0.939
8	CC	—	—	—	—
	CO		—	0.698	0.003
	EC			—	—
	IM				0.464
Tissue	Week	2	4	8
CC	1	0.062	—	—
	2		—	—
	4			—
CO	1	0.587	0.963	0.561
	2		0.312	0.998
	4			0.245
EC	1	0.310	—	—
	2		—	—
	4			—
IM	1	0.012	0.056	0.009
	2		0.308	0.986
	4			0.141
WB	1	0.522	0.221	0.088
	2		0.782	0.251
	4			0.621

(c) Crystallinity

Week	Tissue	CO	EC	IM	WB
1	CC	0.003	0.012	0.013	<0.001
	CO		0.999	0.993	<0.001
	EC			0.998	<0.001
	IM				0.690
2	CC	0.040	0.124	0.017	0.084
	CO		0.987	0.084	0.744
	EC			0.273	0.999
	IM				<0.001
4	CC	—	—	—	—
	CO		—	0.659	0.641
	EC			—	—
	IM				0.979
8	CC	—	—	—	—
	CO		—	0.468	<0.001
	EC			—	—
	IM				0.002
Tissue	Week	2	4	8
CC	1	0.337	—	—
	2		—	—
	4			—
CO	1	0.827	0.999	0.999
	2		0.917	0.870
	4			0.999
EC	1	0.909	—	—
	2		—	—
	4			—
IM	1	0.417	0.999	0.985
	2		0.456	0.603
	4			0.995
WB	1	<0.001	0.878	0.013
	2		0.842	<0.001
	4			0.129

(d) Acid phosphate substitution

Week	Tissue	CO	EC	IM	WB
1	CC	<0.001	0.005	<0.001	<0.001
	CO		<0.001	<0.001	<0.001
	EC			0.363	0.653
	IM				0.756
2	CC	<0.001	0.488	<0.001	0.008
	CO		<0.001	<0.001	<0.001
	EC			<0.001	0.016
	IM				0.534
4	CC	—	—	—	—
	CO		—	0.079	<0.001
	EC			—	—
	IM				0.254
8	CC	—	—	—	—
	CO		—	0.417	<0.001
	EC			—	—
	IM				0.015
Tissue	Week	2	4	8
CC	1	0.680	—	—
	2		—	—
	4			—
CO	1	0.998	0.998	0.847
	2		0.999	0.892
	4			0.870
EC	1	0.645	—	—
	2		—	—
	4			—
IM	1	<0.001	<0.001	<0.001
	2		0.066	0.035
	4			0.984
WB	1	0.002	<0.001	<0.001
	2		0.041	0.017
	4			0.991

(e) Collagen maturity/cross-link ratio

Week	Tissue	CO	EC	IM	WB
1	CC	0.120	0.419	0.972	0.005
	CO		0.495	0.094	0.999
	EC			0.663	0.923
	IM				0.019
2	CC	0.471	0.250	0.494	0.799
	CO		0.119	0.999	0.918
	EC			0.089	0.156
	IM				0.891
4	CC	—	—	—	—
	CO		—	0.968	0.958
	EC			—	—
	IM				0.958
8	CC	—	—	—	—
	CO		—	0.038	0.193
	EC			—	—
	IM				0.823
Tissue	Week	2	4	8
CC	1	0.855	—	—
	2		—	—
	4			—
CO	1	0.999	0.256	0.723
	2		0.234	0.831
	4			0.024
EC	1	0.060	—	—
	2		—	—
	4			—
IM	1	0.020	<0.001	0.561
	2		0.203	0.445
	4			0.008
WB	1	0.879	0.276	0.665
	2		0.092	0.980
	4			0.044

Statistically significant values are shown in bold. CO = cortical bone; CC = calcified cartilage; EC = endochondral bone; IM = intramembranous bone; WB = woven bone. Times are weeks after fracture.