894510.fig.002a
(a)
894510.fig.002b
(b)
Figure 2: Expression of SOCS36E is a proxy for JAK/STAT pathway activity and can be used as a pathway reporter. (a) Double fluorescent in situ hybridization demonstrates the association between the expression domains of upd (top and red) and socs36E (middle and green) within a stage 13 embryo. (b) Late third instar wing imaginal disc expressing the 10xSTAT-GFP reporter construct (green) in regions of high JAK/STAT activity that correspond to upd mRNA expression domains [37]. DNA (blue) outlines wing disc morphology.