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Journal of Toxicology
Volume 2012 (2012), Article ID 843787, 6 pages
Mining the Virgin Land of Neurotoxicology: A Novel Paradigm of Neurotoxic Peptides Action on Glycosylated Voltage-Gated Sodium Channels
Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Nanchen Road 333, Shanghai 200444, China
Received 15 February 2012; Revised 17 April 2012; Accepted 24 May 2012
Academic Editor: Maria Elena de Lima
Copyright © 2012 Zhirui Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Voltage-gated sodium channels (VGSCs) are important membrane protein carrying on the molecular basis for action potentials (AP) in neuronal firings. Even though the structure-function studies were the most pursued spots, the posttranslation modification processes, such as glycosylation, phosphorylation, and alternative splicing associating with channel functions captured less eyesights. The accumulative research suggested an interaction between the sialic acids chains and ion-permeable pores, giving rise to subtle but significant impacts on channel gating. Sodium channel-specific neurotoxic toxins, a family of long-chain polypeptides originated from venomous animals, are found to potentially share the binding sites adjacent to glycosylated region on VGSCs. Thus, an interaction between toxin and glycosylated VGSC might hopefully join the campaign to approach the role of glycosylation in modulating VGSCs-involved neuronal network activity. This paper will cover the state-of-the-art advances of researches on glycosylation-mediated VGSCs function and the possible underlying mechanisms of interactions between toxin and glycosylated VGSCs, which may therefore, fulfill the knowledge in identifying the pharmacological targets and therapeutic values of VGSCs.
In neurons and most excitable cells, multiform action potentials driven by depolarizing neuronal firing are considered to be accounted by spatiotemporal activation and integral performances of tissue-specific VGSCs . Generally, VGSCs consist of an α subunit (260 kDa) and several auxiliary β subunits (β1–β4, 33–38 kDa). The α subunit is well organized in four homologous domains (DI–DIV) which contain six transmembrane segments each (S1–S6). The hairpin-like loop between S5 and S6 segments region is functioned as ion-permeable pore . Notably, both α and β subunits are highly glycosylated cross-membrane proteins [3–7] (Figure 1).
The most common form of glycosylation sites on VGSCs protein is mainly composed by N-linked sialic acids, . Estimation indicates that 15%–40% of the total VGSC α subunit molecular weight is carbohydrate [6, 7, 9]. Approximately 40%–45% of the added carbohydrate residues are sialic acid moieties, resulting in the addition of an estimated 100 sialic acid residues per subunit molecule [6, 9]. Up to now, through bioinformatics prediction, there have been found tens of potential extracellular glycosylation sites located mainly within the pore region lining between DI S5-S6 in α subunit of VGSCs, less of which, however, have been funtionally characterized [5, 10, 11]. Far less than α subunit, only three of the four N-linked glycosylation sites present in the N terminus of β subunits are thought to be glycosylated in the mature protein [5, 12].
Glycosylation has long be known to participate in regulation of functional expression of channels, such as folding, trafficking, and membrane-insert localization, but also influence electrophysiological properties [13–16]. Inhibition of N-linked glycosylation altered the voltage dependence of channel gating of Kv1.1 and KvLQT/minK (IsK) and the open probability of the renal outer medullary K+ channel ROMK1 (inward rectifier K+ channel), as well as the pH sensitivity of KvLQT/minK channels [17, 18]. In addition, N-linked glycosylation was found to be capable of increasing the stability of Shaker potassium channels proteins in trafficking from the endoplasmatic reticulum to the Golgi [13, 15]. Comparably, the information of glycosylation on modulating VGSCs function was less approached. The current knowledge about the modulation of glycosylation on VGSCs is mainly focused on reducing the voltage-dependent gating sensitivity, and thereby lowering the AP threshold at neuronal network level [19–21]. But questions like (1) how does glycosylation control the local static milieu on the surface of VGSCs protein which is attributed to the state of cell membrane? (2) what about the subtype-specific modulation of glycosylation on VGSCs? (3) does each glycosylation site modulate equally or synergically on the VGSCs gating? still appear as difficult tasks to work out.
To address the above problems, one may resort to segment-swap chimera construction or glycosylation-deficient cells to reduce the level of glycosylation [10, 22]. However, such methods may still bring about the unexpected artificial factors. More importantly, the complexity of extracellular environment and structure of channel protein itself may also disturb the reliability. Therefore, one prospective and efficient way is to find out the subtype-specific glycosylation modulators.
Natural toxic polypeptides originated from various venomous animals are deemed to be specifically targeting on VGSCs by either lowering the threshold for activation or delaying the inactivation process [23–26]. To date, there have been found six receptor sites of these toxins on VGSCs, some of which are even residing in the overlapped region of glycosylated sites on VGSCs [23, 26–28]. Meanwhile, the pharmacological studies have demonstrated that the binding of these toxins and their targets is highly subtype specific [26, 27, 29]. Thus, they are hopefully utilized as efficient tools to more precisely uncover the role of glycosylation on VGSCs gating and the overall performances on channel pathology in clinical therapy.
2. Mechanism of Glycosylation to the Voltage Dependence of VGSCs Gating
A general understanding about the physiological function of glycosylation on VGSCs is to control the voltage sensitivity in a channel through the number of sialic acids residing on . Once these sialic acids are removed by deglycosyslated reagents, such as tunicamycin and neuraminidase, the voltage-dependent activation will shift to a more depolarized direction and thereby raising the threshold for AP generation [7, 22]. Currently, one commonly accepted notion is that it was negative charges brought about by a significant content of sialic acid residues on the glycosylation sites of the extracellular region that caused the hyperpolarized voltage dependence of gating . However, some reports have suggested that various Navα subunits are differently glycosylated/sialylated even expressed in the same cell line . This difference in α subunit sialylation directly and differently alters channel gating . Here, two mechanisms describing the differential modulation of glycosylation on VGSCs gating are discussed below.
2.1. “Subtype-Specific” Mechanism
It is well known that VGSCs have nine tissue or developmentally distinct subtypes, named as Nav1.1 to Nav1.9, each of which has been found to be responsible for functionally diverse electric activities within the specific region they are expressed . Recent researches have indicated that different VGSC subtypes have differential responses to the glycosylation.
Deglycosylated Nav1.4 could result in a depolarizing shift in both voltage-dependent activation and inactivation. Comparatively, deglycosylated Nav1.5 could lead to a depolarized voltage-dependent activation but not inactivation [20, 21]. By contrast, deglycosylation could only shift the midpoint of steady-state inactivation of Nav1.9 in adult small DRG neurons to more depolarized potentials . Gating of Nav1.2 and Nav1.7 could not be significantly affected by deglycosylation . Our recent work found that the steady-state activation curve of deglycosylated Nav1.3 was depolarized to a more positive direction, while inactivation curve was negatively shifted . Thus far, glycosylation is capable of modulating VGSCs gating to various extents.
There was a report which attributed such differential modulation of glycosylation to distinct extent of glycosylation of each VGSC subtype. Immunoblot data suggested that Nav1.4 is more glycosylated than Nav1.5 . As a matter of fact, Nav1.1–Nav1.4, are heavily glycosylated (about 15~30%), whilst Nav1.5 and Nav1.9 are barely glycosylated (~5%) . Accordingly, it was indicated that Nav1.4 voltage-dependent gating parameters are significantly and essentially uniformly altered by sialic acid than that of Nav1.5. For the reason that the external surface of one VGSC α-subunit was estimated to have about 110–130 negative charges composed by the sialic acids (amount to about 40% of total carbohydrate in VGSC) , it is likely that sialic acid alters the electric field sensed by the gating mechanism of the channel . That is, a higher level of glycosylation in VGSC may lead to more depolarized shift in voltage-dependent gating when deglycosylated. As a consequence, the deglycosylated VGSCs may require larger depolarizing stimulus to activate.
2.2. “Cell-Specific” Mechanism
One seemly contradictory notion against the “subtype-specific” mechanism was that it was because of certain internal environment of different cells types which differentially produce sialylated proteins leading to a spectrum of Nav functional sialic acid levels that directly modulate channel gating. Hence, such mechanism was termed as “cell specific” [19, 32].
“Cell-specific” may arise from two compensated factors. One is that the glycosylation extent in certain VGSC protein may vary throughout the developmental stages: for example, Na+ currents in adult rat cortical and dorsal root ganglion neurons are less sensitive to sialic acid than Na+ currents from neonatal neurons [20, 34]. Adult ventricular myocyte VGSCs were more heavily sialylated and gated at more hyperpolarized potentials than they were in neonate ventricular myocyte VGSCs. One possible explanation for this increased sialylation would be a chronic increase in sialyltransferase activity in the developing ventricles .
Another aspect resulting in differential glycosylation came from the tissue- or cell-type-specific proteins that modify the number of sialic acids on the surface of VGSCs. One recent work have suggested that the modulation of glycosylation may display a rather complex profile with the combinatorial link with VGSC β-subunit: for example, when β1 subunit was coexpressed with Nav1.2, Nav1.4, Nav1.5, and Nav1.7, the extent of hyperpolarized shift in voltage-dependent activation has the following order: Nav1.7 > Nav1.5 > Nav1.2 > Nav1.4, where Nav1.4 was less modulated by β1 subunit, which indicated an essentially saturating level of functional sialic acids. On the contrary, Nav1.7 alone contain the least functional sialic acids in DIS5-S6, and therefore the levels of functional sialic acids increase, causing the most remarkably hyperpolarization in voltage-dependent activation when coexpressed with β1 subunit .
3. Novel Paradigm of Interaction between VGSCs and Neurotoxic Peptides
Since the distinct binding affinity with VGSCs, natural toxins such as that from marine animals (saxitoxin, sea anemone) and arthropods (scorpion toxins, spider toxins) have long been applied to investigate the structure-function relationship of VGSCs and regarded as pharmacological templates for developing therapeutic leads [23–26, 35].
However, there have been paradoxes seen in contact cells and in vitro studies on VGSCs. For instance, BmK I, a site-3-specific modulator of VGSCs from scorpion Buthus martensii Karsch (BmK) was capable to prevent the inactivation of Nav1.2 and produce persistent current, which may account for BmK I-induced epileptiform responses in rats [36–39]. However, the specific binding of BmK I to the VGSCs in rat brain synaptosomes (mainly Nav1.2) was undetectable . Although the contradictory modulation of BmK I between VGSCs-rich synaptosomes and heterologously expressed Nav1.2 may somewhat attribute to lack of BmK I-sensitive VGSCs , the complex intracellular enzymatic context of synaptosomes stimulated us to speculate the involvement of glycosylation on modulating sensitivity of VGSCs to BmK I.
In our recent researches, it was found that BmK I could distinctively modulate glycosylated/deglycosylated Nav1.2 expressed in oocytes. The voltage dependent activation of deglycosylated Nav1.2 was significantly shifted to more negative direction by BmK I, contrary to the little effects on the glycosylated ones. This research indicated that the glycosylated sites on VGSCs may act as umbrellas to shield the interaction sites of VGSCs with BmK I, and if biochemically removed, may facilitate the binding of BmK I to the receptor sites (Figure 2).
Coincidentally, as a receptor site-3-specific modulator, BmK I has been suggested to be capable of binding to the region where glycosylation sites also reside [37, 40]. It has been indicated that the extracellular loops between transmembrane segments S5 and S6 in domain I of the α-subunit are involved in the formation of receptor site 3 . In addition, several antibodies that recognize the extracellular loops between transmembrane S5 and S6 in domains I and IV prevent α-scorpion toxin binding to receptor site 3, suggesting involvement of amino acid residues in these locations . All these clues may potentially support the notion that there exists interaction between glycosylation sites and BmK I, by which the binding affinities of BmK I to VGSCs may be altered if the channel was decosylated.
Similar phenomenon can also be referred to the mode of actions of sulfamethoxazole (SMX) on HERG channel accompanying with a mutant subunit MiRP1 (T8A). Under normal conditions, the carbohydrates were attached to shield the variable receptor and thus impairing the SMX binding. Conversely, in channels formed with MiRP1 T8A mutants that are deficient in glycosylated sites by mutation, SMX accessibility to the receptor is facilitated by the absence of the oligosaccharide groups .
Likewise, Catterall et al. in 1987 have concluded the possible mechanism to explain the lack of binding of saxitoxin, one VGSCs-specific blocker, to the glycosylated VGSCs: the negative surface charges on the glycosylated sodium channel are expected to increase the local concentration of Na+ near the extracellular opening of its transmembrane pore and to increase the local concentration of a cationic ligand like saxitoxin near its receptor site. The lack of effect of inhibition of sialylation with castanospermine on saxitoxin binding indicates that the saxitoxin receptor site is located distance from the negative surface charges contributed by sialic acid residues or is insulated from their effects by the protein structure .
Several lines of evidence suggest that regulation of the level of sialylation is a powerful mechanism to control the surface charge of channels as well as neuronal channel pathology [27, 43–45]: for example, long QT syndrome (LQT, a cardiac disease relate to HERG) is also caused by the mutation of glycosylated sites in HERG . It was reported that glycosylation is essential for the biosynthesis and the maintenance of functional VGSCs in fibroblastoma cells . Additionally, one of main pathogenesis resulting in sinus arrhythmia is insufficient level of glycosylation in cardiac VGSCs . Moreover, some studies showed that the chronic pain is related to the increase of glycosylation in neuron membrane in nerve injury sites . Finally, an increase in sialic acid-negative surface charge resulting in reduced AP threshold and increased excitability could be one of the important factors of the pathogenesis of epilepsy associated with these inherited disorders .
The researches on detecting the modulation of glycosylation on VGSCs function are subjected to two major challenges. (1) There still lack of specific tools to precisely and efficiently modify the level of glycosylation on VGSCs, which may result in ambiguous observations in the real studies. Maybe some bioinformatics predictions could provide the auxiliary clues to compensate this problem. However, it remains to be more cautious in treating the computer-assisted data even until some site-directed evidence has been conducted. (2) The static alteration in glycosylation sites can restructure the receptor sites in VGSCs where some potential specific drugs may bind with. Thus, it is a critical task to discriminate the possible disturbance of glycosylation when treating with some seemly inefficient drugs targeting on VGSCs.
As sodium channel-specific modulators and neurotoxins have great potentials to probe the intriguing subtle structural variations in VGSCs found across different tissues and species. However, the pharmacological sensitivity of VGSCs toward toxins, seen in Section 3, may be subject to differential modulation due to the glycosylation of VGSC protein itself, leading to the difficulties in observing the actual interactions. Hence, a broader view of how neurotoxins modulate neuronal activities and thereby valuable information regarding to VGSCs as therapeutic targets could be obtained until more precise details about the role of glycosylation in determining toxin-channel interaction be deduced.
This study was supported by the National Basic Research Program of China (2010CB529806), partially by Grants from National Natural Science Foundation of China (31171064), Key Research Program of Science and Technology Commissions of Shanghai Municipality (11JC1404300), and Leading Academic Discipline Project of Shanghai Municipal Education Commission (J50108).
- W. A. Catterall, “Structure and function of voltage-gated ion channels,” Annual Review of Biochemistry, vol. 64, pp. 493–531, 1995.
- A. L. Goldin, “Evolution of voltage-gated Na+ channels,” Journal of Experimental Biology, vol. 205, no. 5, pp. 575–584, 2002.
- S. A. Cohen and L. K. Levitt, “Partial characterization of the rH1 sodium channel protein from rat heart using subtype-specific antibodies,” Circulation Research, vol. 73, no. 4, pp. 735–742, 1993.
- D. Gordon, D. Merrick, D. A. Wollner, and W. A. Catterall, “Biochemical properties of sodium channels in a wide range of excitable tissues studied with site-directed antibodies,” Biochemistry, vol. 27, no. 18, pp. 7032–7038, 1988.
- D. J. Messner and W. A. Catterall, “The sodium channel from rat brain. Separation and characterization of subunits,” Journal of Biological Chemistry, vol. 260, no. 19, pp. 10597–10604, 1985.
- R. H. Roberts and R. L. Barchi, “The voltage-sensitive sodium channel from rabbit skeletal muscle. Chemical characterization of subunits,” Journal of Biological Chemistry, vol. 262, no. 5, pp. 2298–2303, 1987.
- J. W. Schmidt and W. A. Catterall, “Palmitylation, sulfation, and glycosylation of the alpha subunit of the sodium channel. Role of post-translational modifications in channel assembly,” Journal of Biological Chemistry, vol. 262, no. 28, pp. 13713–13723, 1987.
- R. G. Spiro, “Glycoproteins,” Advances in Protein Chemistry, vol. 27, pp. 349–467, 1973.
- J. A. Miller, W. S. Agnew, and S. R. Levinson, “Principal glycopeptide of the tetrodotoxin/saxitoxin binding protein from Electrophorus electricus: isolation and partial chemical and physical characterization,” Biochemistry, vol. 22, no. 2, pp. 462–470, 1983.
- E. S. Bennett, “Isoform-specific effects of sialic acid on voltage-dependent Na+ channel gating: functional sialic acids are localized to the S5-S6 loop of domain I,” Journal of Physiology, vol. 538, no. 3, pp. 675–690, 2002.
- S. A. Cohen and R. L. Barchi, “Glycoprotein characteristics of the sodium channel saxitoxin-binding component from mammalian sarcolemma,” Biochimica et Biophysica Acta, vol. 645, no. 2, pp. 253–261, 1981.
- L. L. Isom, K. S. De Jongh, D. E. Patton et al., “Primary structure and functional expression of the β1 subunit of the rat brain sodium channel,” Science, vol. 256, no. 5058, pp. 839–842, 1992.
- N. F. de Souza and S. M. Simon, “Glycosylation affects the rate of traffic of the shaker potassium channel through the secretory pathway,” Biochemistry, vol. 41, no. 38, pp. 11351–11361, 2002.
- Q. Gong, C. L. Anderson, C. T. January, and Z. Zhou, “Role of glycosylation in cell surface expression and stability of HERG potassium channels,” American Journal of Physiology, vol. 283, no. 1, pp. H77–H84, 2002.
- R. Khanna, M. P. Myers, M. Lainé, and D. M. Papazian, “Glycosylation increases potassium channel stability and surface expression in mammalian cells,” Journal of Biological Chemistry, vol. 276, no. 36, pp. 34028–34034, 2001.
- K. Petrecca, R. Atanasiu, A. Akhavan, and A. Shrier, “N-linked glycosylation sites determine HERG: channel surface membrane expression,” Journal of Physiology, vol. 515, pp. 41–48, 1999.
- L. C. Freeman, J. J. Lippold, and K. E. Mitchell, “Glycosylation influences gating and pH sensitivity of I(sK),” Journal of Membrane Biology, vol. 177, no. 1, pp. 65–79, 2000.
- R. A. Schwalbe, Z. Wang, B. A. Wible, and A. M. Brown, “Potassium channel structure and function as reported by a single glycosylation sequon,” Journal of Biological Chemistry, vol. 270, no. 25, pp. 15336–15340, 1995.
- P. J. Stocker and E. S. Bennett, “Differential sialylation modulates voltage-gated Na+ channel gating throughout the developing myocardium,” Journal of General Physiology, vol. 127, no. 3, pp. 253–265, 2006.
- L. Tyrrell, M. Renganathan, S. D. Dib-Hajj, and S. G. Waxman, “Glycosylation alters steady-state inactivation of sodium channel NAv1.9/NaN in dorsal root ganglion neurons and is developmentally regulated,” Journal of Neuroscience, vol. 21, no. 24, pp. 9629–9637, 2001.
- Y. Zhang, H. A. Hartmann, and J. Satin, “Glycosylation influences voltage-dependent gating of cardiac and skeletal muscle sodium channels,” Journal of Membrane Biology, vol. 171, no. 3, pp. 195–207, 1999.
- D. Isaev, E. Isaeva, T. Shatskih et al., “Role of extracellular sialic acid in regulation of neuronal and network excitability in the rat hippocampus,” Journal of Neuroscience, vol. 27, no. 43, pp. 11587–11594, 2007.
- S. Cestele and W. A. Catterall, “Molecular mechanisms of neurotoxin action on voltage-gated sodium channels,” Biochimie, vol. 82, no. 9-10, pp. 883–892, 2000.
- M. E. De Lima, S. G. Figueiredo, A. M. C. Pimenta et al., “Peptides of arachnid venoms with insecticidal activity targeting sodium channels,” Comparative Biochemistry and Physiology C, vol. 146, no. 1-2, pp. 264–279, 2007.
- C. Goudet, C. W. Chi, and J. Tytgat, “An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch,” Toxicon, vol. 40, no. 9, pp. 1239–1258, 2002.
- X. P. Zuo and Y. H. Ji, “Molecular mechanism of scorpion neurotoxins acting on sodium channels: insight into their diverse selectivity,” Molecular Neurobiology, vol. 30, no. 3, pp. 265–278, 2004.
- M. Stevens, S. Peigneur, and J. Tytgat, “Neurotoxins and their binding areas on voltage-gated sodium channels,” Frontiers in Pharmacology, vol. 2, article 71, 2011.
- F. J. Tejedor and W. A. Catterall, “Site of covalent attachment of α-scorpion toxin derivatives in domain I of the sodium channel α subunit,” Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 22, pp. 8742–8746, 1988.
- Z. R. Liu, P. Ye, and Y. H. Ji, “Exploring the obscure profiles of pharmacological binding sites on voltage-gated sodium channels by BmK neurotoxins,” Protein Cell, vol. 2, pp. 437–444, 2011.
- E. Bennett, M. S. Urcan, S. S. Tinkle, A. G. Koszowski, and S. R. Levinson, “Contribution of sialic acid to the voltage dependence of sodium channel gating: a possible electrostatic mechanism,” Journal of General Physiology, vol. 109, no. 3, pp. 327–343, 1997.
- W. A. Catterall, “From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels,” Neuron, vol. 26, no. 1, pp. 13–25, 2000.
- D. Johnson, M. L. Montpetit, P. J. Stocker, and E. S. Bennett, “The sialic acid component of the beta1 subunit modulates voltage-gated sodium channel function,” Journal of Biological Chemistry, vol. 279, no. 43, pp. 44303–44310, 2004.
- Q. Xu, H. W. Cheng, H. Q. He et al., “Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel,” Neuroscience Bulletin, vol. 24, no. 5, pp. 283–287, 2008.
- C. Zona, F. Eusebi, and R. Miledi, “Glycosylation is required for maintenance of functional voltage-activated channels in growing neocortical neurons of the rat,” Proceedings of the Royal Society B, vol. 239, no. 1295, pp. 119–127, 1990.
- J. C. Rogers, Y. Qu, T. N. Tanada, T. Scheuer, and W. A. Catterall, “Molecular determinants of high affinity binding of α-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the Na+ channel α subunit,” Journal of Biological Chemistry, vol. 271, no. 27, pp. 15950–15962, 1996.
- Z. T. Bai, R. Zhao, X. Y. Zhang, J. Chen, T. Liu, and Y. H. Ji, “The epileptic seizures induced by BmK I, a modulator of sodium channels,” Experimental Neurology, vol. 197, no. 1, pp. 167–176, 2006.
- H. He, Z. Liu, B. Dong, J. Zhou, H. Zhu, and Y. Ji, “Molecular determination of selectivity of the site 3 modulator (BmK I) to sodium channels in the CNS: a clue to the importance of Nav1.6 in BmK I-induced neuronal hyperexcitability,” Biochemical Journal, vol. 431, no. 2, pp. 289–298, 2010.
- Y. H. Ji, P. Mansuelle, S. Terakawa et al., “Two neurotoxins (BMK I and BMK II) from the venom of the scorpion Buthus martensi Karsch: purification, amino acid sequences and assessment of specific activity,” Toxicon, vol. 34, no. 9, pp. 987–1001, 1996.
- M. Tan, M. M. Zhu, Y. Liu, H. W. Cheng, and Y. H. Ji, “Effects of BmK AS on Nav1.2 expressed in Xenopus laevis oocytes,” Cell Biology and Toxicology, vol. 24, no. 2, pp. 143–149, 2008.
- Y. J. Li and Y. H. Ji, “Binding characteristics of BmK I, an α-like scorpion neurotoxic polypeptide, on cockroach nerve cord synaptosomes,” Journal of Peptide Research, vol. 56, no. 4, pp. 195–200, 2000.
- W. J. Thomsen and W. A. Catterall, “Localization of the receptor site for α-scorpion toxins by antibody mapping: implications for sodium channel topology,” Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 24, pp. 10161–10165, 1989.
- K. H. Park, S. M. Kwok, C. Sharon, R. Berga, and F. Sesti, “N-glycosylation-dependent block is a novel mechanism for drug-induced cardiac arrhythmia,” The FASEB Journal, vol. 17, no. 15, pp. 2308–2309, 2003.
- A. Akhavan, “N- and O-linked glycosylation coordinate cell-surface localization of a cardiac potassium channel,” The Journal of Physiology, vol. 589, pp. 4647–4648, 2011.
- M. K. Hall, T. A. Cartwright, C. M. Fleming, and R. A. Schwalbe, “Importance of glycosylation on function of a potassium channel in neuroblastoma cells,” PLoS ONE, vol. 6, no. 4, Article ID e19317, 2011.
- C. A. Satler, M. R. Vesely, P. Duggal, G. S. Ginsburg, and A. H. Beggs, “Multiple different missense mutations in the pore region of HERG in patients with long QT syndrome,” Human Genetics, vol. 102, no. 3, pp. 265–272, 1998.
- A. Pabon, K. W. Chan, J. L. Sui, X. Wu, D. E. Logothetis, and W. B. Thornhill, “Glycosylation of GIRK1 at Asn119 and ROMK1 at Asn117 has different consequences in potassium channel function,” Journal of Biological Chemistry, vol. 275, no. 39, pp. 30677–30682, 2000.
- C. A. Ufret-Vincenty, D. J. Baro, W. J. Lederer, H. A. Rockman, L. E. Quiñones, and L. F. Santana, “Role of sodium channel deglycosylation in the genesis of cardiac arrhythmias in heart failure,” Journal of Biological Chemistry, vol. 276, no. 30, pp. 28197–28203, 2001.
- X. L. Zhang, X. Q. Peng, Y. L. Jing, W. R. Xie, and Y. K. Xie, “Sialic acid contributes to generation of ectopic spontaneous discharges in rats with neuropathic pain,” Neuroscience Letters, vol. 346, no. 1-2, pp. 65–68, 2003.