Production of the Growth Factors GM-CSF, G-CSF, and VEGF by Human Peripheral Blood Cells Induced with Metal Complexes of Human Serum γ-Globulin Formed with Copper or Zinc Ions

Figure 1

Production of GM-CSF by normal human PBC induced with human serum γ-globulin metal complexes formed with copper or zinc ions, with control γ-globulins, and with copper and zinc ions used alone (𝑀±𝑚,𝑛=4). Induction for 48 hrs at 37°C. In the ordinate axis: concentration of GM-CSF, pg/ml. Here and at Figures 2 and 3: 1—control γ-globulin for the zinc complex, 2—γ-globulin complex formed with zinc ions, 3—zinc ions used alone, 4—control γ-globulin for the copper complex, 5—γ-globulin complex formed with copper ions, and 6—copper ions used alone. Here and at the Figures 2 and 3: the concentrations were calculated from the data obtained with using primary (not diluted) state of the samples. ^∗𝑃<0.05 compared with zinc ions used alone (3) or with γ-globulin complex formed with copper ions (5); ^∗∗𝑃<0.01 compared with control γ-globulin for the zinc complex (1) or with γ-globulin complex formed with zinc ions (2); ^∗∗∗𝑃<0.001 compared with γ-globulin complex formed with zinc ions (2) or with zinc ions used alone (3), with control γ-globulin for the copper complex (4) and with γ-globulin complex formed with copper ions (5).