P2Y₁₂ Receptor on the Verge of a Neuroinflammatory Breakdown

Figure 2

P2Y₁₂ receptor in dissociated and organotypic primary cultures. (a) Mouse primary cortical microglia were subjected to immunofluorescence and confocal analysis with phalloidin (green, merged field) and P2Y₁₂ receptor antibodies (red, insets and merged) and Hoechst (white, merged). Scale bars in insets: 20 μm. (b) Double immunofluorescence and confocal analysis of primary rat mature (OL) and precursor (OPC) oligodendrocytes was performed with antibodies for P2Y₁₂ receptor, MBP, NG2 (Table 1). For intra2 antibodies, lots AN01/02/04/0502/0602 were used. (c) Rat cerebellar organotypic cultures were analyzed by double immunofluorescence and confocal microscopy for intra1 (red) and c-ter (red), highlighting different structures (see also insets), and MBP (green).

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