Biochemical Evidence for a Putative Inositol 1,3,4,5-Tetrakisphosphate Receptor in the Olfactory System of Atlantic Salmon (Salmo salar)
Figure 2
Saturation binding of [3H]IP4 to a plasma membrane rich fraction from salmon olfactory rosettes. Specific binding of IP4 was determined at each of the IP4 concentrations shown. Results are averaged from a single experiment performed in duplicate using olfactory rosettes from 10 Atlantic salmon and are representative of the results of three independent experiments. The inset shows a Scatchard analysis of the binding of IP4 to the plasma membrane rich fraction. Binding data as in Figure 2 was transformed to estimate the and . The data fit a straight line ( = 0.93) indicative of a single class of binding sites.
