Research Article

rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ

Figure 3

Effects of rAAV-mediated gene transfer on the survival of transduced primary human chondrosarcoma cells in vitro. Cells in monolayer culture were transduced as described in Figure 1 or let untreated for up to 20 days to monitor the viable cell numbers (a), cell viability (b), the rates of proliferation (WST-1 assay) (c), the levels of apoptosis (TUNEL assay) (d), and potential cytotoxic responses to the treatment by rAAV (Cytotoxicity Detection Assay LDH) (e).
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(a)
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(b)
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(c)
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(d)
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(e)