rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ
Figure 3
Effects of rAAV-mediated gene transfer on the survival of transduced primary human chondrosarcoma cells in vitro. Cells in monolayer culture were transduced as described in Figure 1 or let untreated for up to 20 days to monitor the viable cell numbers (a), cell viability (b), the rates of proliferation (WST-1 assay) (c), the levels of apoptosis (TUNEL assay) (d), and potential cytotoxic responses to the treatment by rAAV (Cytotoxicity Detection Assay LDH) (e).