Cases 1 and 3: inadequate RNA for RT-PCR. Case 2: no evidence of PAX3/FOXO1A or PAX7/FOXO1A fusion gene by RT-PCR. Case 4: not tested due to the absence of nonembolized tissue
Case 1: normal. Case 2: complex structural and numerical abnormalities with numerous double minutes in most cells; case 3: less complex, with balanced translocation 46,XX,t(5;20)(q31;pl3)
SNP array: complex aneuploid pattern including gains and losses of whole chromosome and an amplification of 12q13-15. FISH analysis showed amplification of HMGA2 and MDM2